Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380884090> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W4380884090 abstract "Abstract Background Isolation of brain‐secreted extracellular vesicles (BEVs) from blood, provides a novel, minimally invasive way to sample brain tissue in individuals along the Alzheimer’s disease (AD) continuum. BEVs in blood demonstrate enormous potential to serve as a novel AD biomarker discovery platform and treatment tool. However, at present, BEV isolation and validation protocols vary from center to center. Given the growing popularity of immunoprecipitation (IP)‐based BEV isolation methods, we assessed the brain‐specificity of reported cell‐surface and cargo proteins associated with this category of methods. Method We performed a systematic evaluation of BEV isolation and validation protocols from 34 published articles investigating biomarker potential of blood‐isolated BEVs in AD. Proteins used for polyethylene glycol‐based precipitation followed by IP‐capture BEV isolation, or as BEV biomarker candidates, were compiled. The proteins were categorized by brain specificity, abundance, extracellular accessibility and extracellular vesicles presence by examining their expression from publicly available datasets in over 60 human tissue types, including 13 central nervous system (CNS) regions and 48 peripheral tissues. Result We compiled 68 unique IP or biomarker protein candidates. Analyses demonstrated that of these, 22 had extracellular domains (that could be used for BEV isolation), 7 were membrane proteins lacking extracellular domains, and the remaining were intracellular proteins. Moreover, >50% of the compiled proteins were present in extracellular vesicle datasets, >20% were considered highly CNS specific, and ∼60% demonstrated moderate‐to‐high CNS abundance (Fig.1). Furthermore, in addition to confirming that some proteins widely used for IP‐based BEV isolation are CNS‐enriched (e.g., L1CAM, Fig. 1B), we also identified, at least, 5 additional proteins with higher CNS abundance and specificity, extracellular domains, and known to be present in extracellular vesicles. Conclusion Consensus harmonization of BEV isolation and validation protocols are essential steps in blood‐isolated BEV biomarker work in AD, and is advocated by the Alzheimer’s Association BBB‐PIA. This harmonization of BEV protocols is crucial, given the growing interest in developing minimally‐invasive AD biomarker discovery tools, and the increasing number of researchers worldwide conducting BEV biomarker work. Moreover, the additional proteins identified by the EWG‐BBB‐PIA will lead to better IP‐based BEV isolation from blood." @default.
- W4380884090 created "2023-06-17" @default.
- W4380884090 creator A5003550473 @default.
- W4380884090 creator A5011412758 @default.
- W4380884090 creator A5013872335 @default.
- W4380884090 creator A5014243339 @default.
- W4380884090 creator A5029146569 @default.
- W4380884090 creator A5059897855 @default.
- W4380884090 creator A5076437087 @default.
- W4380884090 creator A5084417068 @default.
- W4380884090 date "2023-06-01" @default.
- W4380884090 modified "2023-10-05" @default.
- W4380884090 title "Consensus harmonization of brain‐secreted extracellular vesicles isolation and validation protocols for blood biomarker work in Alzheimer’s disease: An international overview" @default.
- W4380884090 doi "https://doi.org/10.1002/alz.061903" @default.
- W4380884090 hasPublicationYear "2023" @default.
- W4380884090 type Work @default.
- W4380884090 citedByCount "0" @default.
- W4380884090 crossrefType "journal-article" @default.
- W4380884090 hasAuthorship W4380884090A5003550473 @default.
- W4380884090 hasAuthorship W4380884090A5011412758 @default.
- W4380884090 hasAuthorship W4380884090A5013872335 @default.
- W4380884090 hasAuthorship W4380884090A5014243339 @default.
- W4380884090 hasAuthorship W4380884090A5029146569 @default.
- W4380884090 hasAuthorship W4380884090A5059897855 @default.
- W4380884090 hasAuthorship W4380884090A5076437087 @default.
- W4380884090 hasAuthorship W4380884090A5084417068 @default.
- W4380884090 hasBestOaLocation W43808840901 @default.
- W4380884090 hasConcept C104317684 @default.
- W4380884090 hasConcept C145059251 @default.
- W4380884090 hasConcept C20518536 @default.
- W4380884090 hasConcept C2781197716 @default.
- W4380884090 hasConcept C28406088 @default.
- W4380884090 hasConcept C2908689518 @default.
- W4380884090 hasConcept C2992929900 @default.
- W4380884090 hasConcept C55493867 @default.
- W4380884090 hasConcept C70721500 @default.
- W4380884090 hasConcept C86803240 @default.
- W4380884090 hasConcept C95444343 @default.
- W4380884090 hasConceptScore W4380884090C104317684 @default.
- W4380884090 hasConceptScore W4380884090C145059251 @default.
- W4380884090 hasConceptScore W4380884090C20518536 @default.
- W4380884090 hasConceptScore W4380884090C2781197716 @default.
- W4380884090 hasConceptScore W4380884090C28406088 @default.
- W4380884090 hasConceptScore W4380884090C2908689518 @default.
- W4380884090 hasConceptScore W4380884090C2992929900 @default.
- W4380884090 hasConceptScore W4380884090C55493867 @default.
- W4380884090 hasConceptScore W4380884090C70721500 @default.
- W4380884090 hasConceptScore W4380884090C86803240 @default.
- W4380884090 hasConceptScore W4380884090C95444343 @default.
- W4380884090 hasIssue "S2" @default.
- W4380884090 hasLocation W43808840901 @default.
- W4380884090 hasOpenAccess W4380884090 @default.
- W4380884090 hasPrimaryLocation W43808840901 @default.
- W4380884090 hasRelatedWork W2257805971 @default.
- W4380884090 hasRelatedWork W2760292854 @default.
- W4380884090 hasRelatedWork W2780439829 @default.
- W4380884090 hasRelatedWork W2905073085 @default.
- W4380884090 hasRelatedWork W2917685500 @default.
- W4380884090 hasRelatedWork W3029637598 @default.
- W4380884090 hasRelatedWork W3047759831 @default.
- W4380884090 hasRelatedWork W4366984173 @default.
- W4380884090 hasRelatedWork W81983803 @default.
- W4380884090 hasRelatedWork W824570847 @default.
- W4380884090 hasVolume "19" @default.
- W4380884090 isParatext "false" @default.
- W4380884090 isRetracted "false" @default.
- W4380884090 workType "article" @default.