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- W4380884804 abstract "Natural laccases are multicopper oxidases widely applied in enzymatic biotransformation. However, using natural laccases in real-world settings is expensive and usually hampered by their stability. Most reported laccase-like nanozymes are limited to copper-containing, while other materials have yet to receive sufficient attention. Herein, platinum (Pt) nanoparticles (NPs) have been constructed as a laccase-like nanozyme using sodium carboxymethylcellulose (CMC-PtNPs). The as-prepared CMC-PtNPs have small particle size (3.8 ± 0.03 nm), good dispersion, and clear interaction mode between polysaccharides and nanozyme. CMC-PtNPs were found to own applicable laccase-like activity by producing O2•− as oxidants to oxidize laccase-related substrates. According to the steady-state dynamic assay, the apparent Km value of CMC-PtNPs was calculated as 0.218 mM. Compared with natural laccase or laccase-like materials, the laccase-like CMC-PtNPs have the advantage of higher substrate affinity and catalytic effect. More significantly, CMC-PtNPs were found to be highly stable at wide-ranged pH and temperature. On a practical level, the adequacy of the developed CMC-PtNPs was also confirmed by the efficient detection of the laccase-related phenolic compounds (e.g., 2,4-dichlorophenol, dopamine, noradrenaline, and adrenaline). A highly linear relationship (R2 = 0.994) was obtained by plotting the absorbance at 510 nm and 2,4-DCP concentration in a range of 6.25 to 225 μM with a low limit of detection (LOD) of 900 nM. Also, the colorimetric sensor exhibited outstanding sensing performance toward other phenolic compounds. Looking forward, this work provides a new design of laccase-like Pt nanozyme and demonstrates its utilization potentiality in nanozyme research, environmental remediation, and other fields." @default.
- W4380884804 created "2023-06-17" @default.
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- W4380884804 date "2023-10-01" @default.
- W4380884804 modified "2023-10-16" @default.
- W4380884804 title "Construction of Platinum Nanozyme by Using Carboxymethylcellulose with Improved Laccase-Like Activity for Phenolic Compounds Detection" @default.
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- W4380884804 doi "https://doi.org/10.1016/j.snb.2023.134165" @default.
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