FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380992028> ?p ?o ?g. }

• W4380992028 endingPage "507" @default.
• W4380992028 startingPage "489" @default.
• W4380992028 abstract "Introduction: Pseudomonas aeruginosa is an ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, P. aeruginosa, and Enterobacter spp.) pathogen and one of the leading etiologies in multiple nosocomial infections. Treatment of P. aeruginosa is becoming increasingly difficult due to its ever-increasing antibiotic resistance trends. This study investigated clinical multidrug resistance (MDR) P. aeruginosa (MDR-PA), their intrinsic resistance determinants, including the presence of chromosomal AmpC β-lactamase (Ampicillinase), decreased expression of outer membrane porin protein OprD and selected acquired β-lactamase resistance genes.&#x0D; Methods: Out of 238 clinical specimens, including urines from urinary tract-infected patients, wound swabs, burn swabs, and catheter aspirates, were collected from two major hospitals in Savar, Dhaka, Bangladesh. Samples were inoculated with Cetrimide agar to isolate presumptive P. aeruginosa. Bacteria were identified by cultural, biochemical characterization, 16S rDNA sequencing, and phylogenetic analysis. Virulence-associated genes of P. aeruginosa, namely, toxA, lasB, and plcH, were identified by polymerase chain reaction (PCR). Antibiotic susceptibilities of the isolates were investigated against ten antibiotics belonging to seven groups by disc-diffusion method followed by a selected minimum inhibitory concentration (MIC) assay. Phenotypic expression of Metallo-β-lactamases (MBLs) production was checked by the double disc synergistic test selectively among the imipenem-resistant isolates. Acquisition of β-lactam resistance trait was examined by PCR detection of bla-genes variants. Mutational loss of the OprD was analyzed by PCR to investigate intrinsic resistance determinants. Phenotypic overexpression of chromosomal AmpC was assayed with the identification of the AmpC gene by PCR. The expression level of OprD was assessed by real-time quantitative PCR (RT-qPCR).&#x0D; Results: Fifty-three P. aeruginosa was identified, with an overall isolation of 22.3% (53/238), where urine remains the most prevalent source. Virulence genes toxA, lasB, and plcH were identified in the isolates of 92.4%, 96.2%, and 94.3%. The highest phenotypic antimicrobial resistance was observed against ampicillin and ceftriaxone (100%), followed by cefotaxime (96%), tetracycline (89%), azithromycin (72%), imipenem (31%), ciprofloxacin (29%), levofloxacin (29%), gentamycin (27%) and ceftazidime (14%). The antibiogram pattern revealed 85% of isolates as multidrug-resistant, while 12% were considered extensively drug-resistant (XDR)-P. aeruginosa. The carriage of β-lactamase genes blaTEM, blaSHV, and blaOXA was detected in 4%, 2%, and 2% cephalosporin-resistant isolates, respectively. Double disc synergistic test revealed 87% of imipenem-resistant isolates expressing MBL-mediated resistance phenomenon. All seven ceftazidime-resistant isolates showed the presence of the AmpC gene with phenotypic overproduction of the AmpC enzyme, indicating AmpC-mediated ceftazidime resistance. Mutational loss of OprD was observed in 12% of phenotypically multidrug-resistant isolates, and RT-qPCR analysis revealed reduced expression of OprD porin protein at various levels in the outer membrane of multidrug-resistant isolates.&#x0D; Conclusions: This study depicts the high prevalence of MDR-PA in clinical specimens in Bangladesh. The identified intrinsic and acquired antimicrobial resistance determinants play synergistic roles in emerging MDR-PA.&#x0D; Bangladesh Journal of Medical Science Vol. 22 No. 03 July’23 Page : 489-507" @default.
• W4380992028 created "2023-06-17" @default.
• W4380992028 creator A5009719216 @default.
• W4380992028 creator A5025308108 @default.
• W4380992028 creator A5027110904 @default.
• W4380992028 creator A5033012443 @default.
• W4380992028 creator A5040414451 @default.
• W4380992028 creator A5049527500 @default.
• W4380992028 creator A5054969127 @default.
• W4380992028 creator A5055171692 @default.
• W4380992028 creator A5079175342 @default.
• W4380992028 creator A5090203018 @default.
• W4380992028 creator A5092189256 @default.
• W4380992028 date "2023-06-16" @default.
• W4380992028 modified "2023-09-27" @default.
• W4380992028 title "Roles of intrinsic and acquired resistance determinants in multidrug-resistant clinical Pseudomonas aeruginosa in Bangladesh" @default.
• W4380992028 doi "https://doi.org/10.3329/bjms.v22i3.66960" @default.
• W4380992028 hasPublicationYear "2023" @default.
• W4380992028 type Work @default.
• W4380992028 citedByCount "0" @default.
• W4380992028 crossrefType "journal-article" @default.
• W4380992028 hasAuthorship W4380992028A5009719216 @default.
• W4380992028 hasAuthorship W4380992028A5025308108 @default.
• W4380992028 hasAuthorship W4380992028A5027110904 @default.
• W4380992028 hasAuthorship W4380992028A5033012443 @default.
• W4380992028 hasAuthorship W4380992028A5040414451 @default.
• W4380992028 hasAuthorship W4380992028A5049527500 @default.
• W4380992028 hasAuthorship W4380992028A5054969127 @default.
• W4380992028 hasAuthorship W4380992028A5055171692 @default.
• W4380992028 hasAuthorship W4380992028A5079175342 @default.
• W4380992028 hasAuthorship W4380992028A5090203018 @default.
• W4380992028 hasAuthorship W4380992028A5092189256 @default.
• W4380992028 hasBestOaLocation W43809920281 @default.
• W4380992028 hasConcept C104317684 @default.
• W4380992028 hasConcept C114851261 @default.
• W4380992028 hasConcept C133936738 @default.
• W4380992028 hasConcept C2776315533 @default.
• W4380992028 hasConcept C2777058267 @default.
• W4380992028 hasConcept C2777637488 @default.
• W4380992028 hasConcept C2779631663 @default.
• W4380992028 hasConcept C501593827 @default.
• W4380992028 hasConcept C523546767 @default.
• W4380992028 hasConcept C54355233 @default.
• W4380992028 hasConcept C547475151 @default.
• W4380992028 hasConcept C60987743 @default.
• W4380992028 hasConcept C86803240 @default.
• W4380992028 hasConcept C89423630 @default.
• W4380992028 hasConcept C94665300 @default.
• W4380992028 hasConceptScore W4380992028C104317684 @default.
• W4380992028 hasConceptScore W4380992028C114851261 @default.
• W4380992028 hasConceptScore W4380992028C133936738 @default.
• W4380992028 hasConceptScore W4380992028C2776315533 @default.
• W4380992028 hasConceptScore W4380992028C2777058267 @default.
• W4380992028 hasConceptScore W4380992028C2777637488 @default.
• W4380992028 hasConceptScore W4380992028C2779631663 @default.
• W4380992028 hasConceptScore W4380992028C501593827 @default.
• W4380992028 hasConceptScore W4380992028C523546767 @default.
• W4380992028 hasConceptScore W4380992028C54355233 @default.
• W4380992028 hasConceptScore W4380992028C547475151 @default.
• W4380992028 hasConceptScore W4380992028C60987743 @default.
• W4380992028 hasConceptScore W4380992028C86803240 @default.
• W4380992028 hasConceptScore W4380992028C89423630 @default.
• W4380992028 hasConceptScore W4380992028C94665300 @default.
• W4380992028 hasIssue "3" @default.
• W4380992028 hasLocation W43809920281 @default.
• W4380992028 hasOpenAccess W4380992028 @default.
• W4380992028 hasPrimaryLocation W43809920281 @default.
• W4380992028 hasRelatedWork W2047085611 @default.
• W4380992028 hasRelatedWork W2374132018 @default.
• W4380992028 hasRelatedWork W2385526059 @default.
• W4380992028 hasRelatedWork W2385766339 @default.
• W4380992028 hasRelatedWork W2615820247 @default.
• W4380992028 hasRelatedWork W2790286024 @default.
• W4380992028 hasRelatedWork W3021121956 @default.
• W4380992028 hasRelatedWork W3032442621 @default.
• W4380992028 hasRelatedWork W4283313781 @default.
• W4380992028 hasRelatedWork W4285796411 @default.
• W4380992028 hasVolume "22" @default.
• W4380992028 isParatext "false" @default.
• W4380992028 isRetracted "false" @default.
• W4380992028 workType "article" @default.