FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380997351> ?p ?o ?g. }

• W4380997351 endingPage "1279" @default.
• W4380997351 startingPage "1279" @default.
• W4380997351 abstract "Increased oxidative stress, dysfunctional cellular clearance, and chronic inflammation are associated with age-related macular degeneration (AMD). Prolyl oligopeptidase (PREP) is a serine protease that has numerous cellular functions, including the regulation of oxidative stress, protein aggregation, and inflammation. PREP inhibition by KYP-2047 (4-phenylbutanoyl-L-prolyl1(S)-cyanopyrrolidine) has been associated with clearance of cellular protein aggregates and reduced oxidative stress and inflammation. Here, we studied the effects of KYP-2047 on inflammation, oxidative stress, cell viability, and autophagy in human retinal pigment epithelium (RPE) cells with reduced proteasomal clearance. MG-132-mediated proteasomal inhibition in ARPE-19 cells was used to model declined proteasomal clearance in the RPEs of AMD patients. Cell viability was assessed using LDH and MTT assays. The amounts of reactive oxygen species (ROS) were measured using 2',7'-dichlorofluorescin diacetate (H2DCFDA). ELISA was used to determine the levels of cytokines and activated mitogen-activated protein kinases. The autophagy markers p62/SQSTM1 and LC3 were measured with the western blot method. MG-132 induced LDH leakage and increased ROS production in the ARPE-19 cells, and KYP-2047 reduced MG-132-induced LDH leakage. Production of the proinflammatory cytokine IL-6 was concurrently alleviated by KYP-2047 when compared with cells treated only with MG-132. KYP-2047 had no effect on autophagy in the RPE cells, but the phosphorylation levels of p38 and ERK1/2 were elevated upon KYP-2047 exposure, and the inhibition of p38 prevented the anti-inflammatory actions of KYP-2047. KYP-2047 showed cytoprotective and anti-inflammatory effects on RPE cells suffering from MG-132-induced proteasomal inhibition." @default.
• W4380997351 created "2023-06-17" @default.
• W4380997351 creator A5006421296 @default.
• W4380997351 creator A5021448617 @default.
• W4380997351 creator A5037753197 @default.
• W4380997351 creator A5041065381 @default.
• W4380997351 creator A5043554503 @default.
• W4380997351 creator A5054448769 @default.
• W4380997351 creator A5056896333 @default.
• W4380997351 creator A5063605821 @default.
• W4380997351 creator A5071846201 @default.
• W4380997351 date "2023-06-15" @default.
• W4380997351 modified "2023-10-01" @default.
• W4380997351 title "The Prolyl Oligopeptidase Inhibitor KYP-2047 Is Cytoprotective and Anti-Inflammatory in Human Retinal Pigment Epithelial Cells with Defective Proteasomal Clearance" @default.
• W4380997351 cites W1523693806 @default.
• W4380997351 cites W1835826588 @default.
• W4380997351 cites W1910308666 @default.
• W4380997351 cites W1964849047 @default.
• W4380997351 cites W2005487633 @default.
• W4380997351 cites W2006344722 @default.
• W4380997351 cites W2007241458 @default.
• W4380997351 cites W2032217161 @default.
• W4380997351 cites W2036700120 @default.
• W4380997351 cites W2037574006 @default.
• W4380997351 cites W2048930707 @default.
• W4380997351 cites W2050599698 @default.
• W4380997351 cites W2051332099 @default.
• W4380997351 cites W2053304083 @default.
• W4380997351 cites W2058128584 @default.
• W4380997351 cites W2062340794 @default.
• W4380997351 cites W2076163934 @default.
• W4380997351 cites W2077721229 @default.
• W4380997351 cites W2086253788 @default.
• W4380997351 cites W2091297212 @default.
• W4380997351 cites W2101958831 @default.
• W4380997351 cites W2112399469 @default.
• W4380997351 cites W2115025243 @default.
• W4380997351 cites W2121065389 @default.
• W4380997351 cites W2125938745 @default.
• W4380997351 cites W2128635872 @default.
• W4380997351 cites W2133673279 @default.
• W4380997351 cites W2140677435 @default.
• W4380997351 cites W2161074777 @default.
• W4380997351 cites W2169069793 @default.
• W4380997351 cites W2264982258 @default.
• W4380997351 cites W2412251503 @default.
• W4380997351 cites W2417468040 @default.
• W4380997351 cites W2517549119 @default.
• W4380997351 cites W2817183627 @default.
• W4380997351 cites W2864177024 @default.
• W4380997351 cites W2871293796 @default.
• W4380997351 cites W2888458308 @default.
• W4380997351 cites W2977458015 @default.
• W4380997351 cites W2991513562 @default.
• W4380997351 cites W3025636602 @default.
• W4380997351 cites W3026560699 @default.
• W4380997351 cites W3033281357 @default.
• W4380997351 cites W3107629007 @default.
• W4380997351 cites W3149626258 @default.
• W4380997351 cites W3160850947 @default.
• W4380997351 cites W4200612130 @default.
• W4380997351 cites W4293247451 @default.
• W4380997351 cites W4293512185 @default.
• W4380997351 cites W4385851958 @default.
• W4380997351 doi "https://doi.org/10.3390/antiox12061279" @default.
• W4380997351 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37372009" @default.
• W4380997351 hasPublicationYear "2023" @default.
• W4380997351 type Work @default.
• W4380997351 citedByCount "1" @default.
• W4380997351 countsByYear W43809973512023 @default.
• W4380997351 crossrefType "journal-article" @default.
• W4380997351 hasAuthorship W4380997351A5006421296 @default.
• W4380997351 hasAuthorship W4380997351A5021448617 @default.
• W4380997351 hasAuthorship W4380997351A5037753197 @default.
• W4380997351 hasAuthorship W4380997351A5041065381 @default.
• W4380997351 hasAuthorship W4380997351A5043554503 @default.
• W4380997351 hasAuthorship W4380997351A5054448769 @default.
• W4380997351 hasAuthorship W4380997351A5056896333 @default.
• W4380997351 hasAuthorship W4380997351A5063605821 @default.
• W4380997351 hasAuthorship W4380997351A5071846201 @default.
• W4380997351 hasBestOaLocation W43809973511 @default.
• W4380997351 hasConcept C104317684 @default.
• W4380997351 hasConcept C1491633281 @default.
• W4380997351 hasConcept C164027704 @default.
• W4380997351 hasConcept C184235292 @default.
• W4380997351 hasConcept C185592680 @default.
• W4380997351 hasConcept C190283241 @default.
• W4380997351 hasConcept C203014093 @default.
• W4380997351 hasConcept C203522944 @default.
• W4380997351 hasConcept C2776151105 @default.
• W4380997351 hasConcept C2776415932 @default.
• W4380997351 hasConcept C2776914184 @default.
• W4380997351 hasConcept C2778024200 @default.
• W4380997351 hasConcept C2780827179 @default.
• W4380997351 hasConcept C51551487 @default.
• W4380997351 hasConcept C53227056 @default.
• W4380997351 hasConcept C55493867 @default.
• W4380997351 hasConcept C86803240 @default.

• next