Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380997658> ?p ?o ?g. }
- W4380997658 endingPage "10131" @default.
- W4380997658 startingPage "10131" @default.
- W4380997658 abstract "The malignity of lung cancer is conditioned by the tumor microenvironment (TME), in which cancer-associated fibroblasts (CAFs) are relevant. In this work, we generated organoids by combining A549 cells with CAFs and normal fibroblasts (NF) isolated from adenocarcinoma tumors. We optimized the conditions for their manufacture in a short time. We evaluated the morphology of organoids using confocal microscopy analysis of F-actin, vimentin and pankeratin. We determined the ultrastructure of the cells in the organoids via transmission electron microscopy and the expression of CDH1, CDH2 and VIM via RT-PCR. The addition of stromal cells induces the self-organization of the organoids, which acquired a bowl morphology, as well as their growth and the generation of cell processes. They also influenced the expression of genes related to epithelial mesenchymal transition (EMT). CAFs potentiated these changes. All cells acquired a characteristic secretory phenotype, with cohesive cells appearing inside the organoids. In the periphery, many cells acquired a migratory phenotype, especially in organoids that incorporated CAFs. The deposit of abundant extracellular matrix could also be observed. The results presented here reinforce the role of CAFs in the progression of lung tumors and could lay the foundation for a useful in vitro pharmacological model." @default.
- W4380997658 created "2023-06-17" @default.
- W4380997658 creator A5021422014 @default.
- W4380997658 creator A5021773626 @default.
- W4380997658 creator A5030887798 @default.
- W4380997658 creator A5033989579 @default.
- W4380997658 creator A5057034660 @default.
- W4380997658 creator A5069278543 @default.
- W4380997658 creator A5074773006 @default.
- W4380997658 creator A5092190347 @default.
- W4380997658 creator A5092190348 @default.
- W4380997658 date "2023-06-14" @default.
- W4380997658 modified "2023-10-18" @default.
- W4380997658 title "Morphological Characterization of Human Lung Cancer Organoids Cultured in Type I Collagen Hydrogels: A Histological Approach" @default.
- W4380997658 cites W1976767579 @default.
- W4380997658 cites W1977524356 @default.
- W4380997658 cites W2033474014 @default.
- W4380997658 cites W2075261412 @default.
- W4380997658 cites W2091336659 @default.
- W4380997658 cites W2117692326 @default.
- W4380997658 cites W2128079038 @default.
- W4380997658 cites W2168631000 @default.
- W4380997658 cites W2168933068 @default.
- W4380997658 cites W2462818054 @default.
- W4380997658 cites W2587816384 @default.
- W4380997658 cites W2612358593 @default.
- W4380997658 cites W2724221128 @default.
- W4380997658 cites W2743943711 @default.
- W4380997658 cites W2754728517 @default.
- W4380997658 cites W2801309958 @default.
- W4380997658 cites W2801676445 @default.
- W4380997658 cites W2891846853 @default.
- W4380997658 cites W2904211489 @default.
- W4380997658 cites W2907188475 @default.
- W4380997658 cites W2917351875 @default.
- W4380997658 cites W2942335981 @default.
- W4380997658 cites W2967862260 @default.
- W4380997658 cites W2971872568 @default.
- W4380997658 cites W2972865130 @default.
- W4380997658 cites W3004517235 @default.
- W4380997658 cites W3005991537 @default.
- W4380997658 cites W3014913757 @default.
- W4380997658 cites W3044822653 @default.
- W4380997658 cites W3091158269 @default.
- W4380997658 cites W3093981785 @default.
- W4380997658 cites W3128646645 @default.
- W4380997658 cites W3131221939 @default.
- W4380997658 cites W3176346185 @default.
- W4380997658 cites W3185300708 @default.
- W4380997658 cites W3199222309 @default.
- W4380997658 cites W4212948459 @default.
- W4380997658 cites W4283023065 @default.
- W4380997658 cites W4293877396 @default.
- W4380997658 cites W4311521690 @default.
- W4380997658 cites W89612929 @default.
- W4380997658 doi "https://doi.org/10.3390/ijms241210131" @default.
- W4380997658 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37373279" @default.
- W4380997658 hasPublicationYear "2023" @default.
- W4380997658 type Work @default.
- W4380997658 citedByCount "1" @default.
- W4380997658 countsByYear W43809976582023 @default.
- W4380997658 crossrefType "journal-article" @default.
- W4380997658 hasAuthorship W4380997658A5021422014 @default.
- W4380997658 hasAuthorship W4380997658A5021773626 @default.
- W4380997658 hasAuthorship W4380997658A5030887798 @default.
- W4380997658 hasAuthorship W4380997658A5033989579 @default.
- W4380997658 hasAuthorship W4380997658A5057034660 @default.
- W4380997658 hasAuthorship W4380997658A5069278543 @default.
- W4380997658 hasAuthorship W4380997658A5074773006 @default.
- W4380997658 hasAuthorship W4380997658A5092190347 @default.
- W4380997658 hasAuthorship W4380997658A5092190348 @default.
- W4380997658 hasBestOaLocation W43809976581 @default.
- W4380997658 hasConcept C121608353 @default.
- W4380997658 hasConcept C142724271 @default.
- W4380997658 hasConcept C147447768 @default.
- W4380997658 hasConcept C16930146 @default.
- W4380997658 hasConcept C185592680 @default.
- W4380997658 hasConcept C189165786 @default.
- W4380997658 hasConcept C198826908 @default.
- W4380997658 hasConcept C203014093 @default.
- W4380997658 hasConcept C204232928 @default.
- W4380997658 hasConcept C2776107976 @default.
- W4380997658 hasConcept C2779013556 @default.
- W4380997658 hasConcept C2909706936 @default.
- W4380997658 hasConcept C3020616263 @default.
- W4380997658 hasConcept C31695470 @default.
- W4380997658 hasConcept C502942594 @default.
- W4380997658 hasConcept C54355233 @default.
- W4380997658 hasConcept C71924100 @default.
- W4380997658 hasConcept C76419328 @default.
- W4380997658 hasConcept C81729549 @default.
- W4380997658 hasConcept C81885089 @default.
- W4380997658 hasConcept C86803240 @default.
- W4380997658 hasConcept C95444343 @default.
- W4380997658 hasConcept C96232424 @default.
- W4380997658 hasConceptScore W4380997658C121608353 @default.
- W4380997658 hasConceptScore W4380997658C142724271 @default.
- W4380997658 hasConceptScore W4380997658C147447768 @default.