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- W4381051388 endingPage "125477" @default.
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- W4381051388 abstract "Natural deep eutectic solvents (NADESs) have been explored to provide a favorable environment for protein stabilization. In this context, NADESs were prepared with the molar ratio of trehalose to betaine ranging from 1:3 to 1:9 (NADES 1-3 to NADES 1-9). There was a strong hydrogen bond interaction between trehalose and betaine, and the interaction weakened with the reduction of trehalose. The NADES 1-7 had good thermal stability (−60–100 °C), low viscosity, and suitable pH (around 7). Trypsin had the highest relative enzyme activity in 50 % (v/v) NADES 1-7 under different temperatures, pH, and storage time. Furthermore, the changes in kinetic parameters indicated that the hydrogen bond environment of 50 % NADES 1-7 increased the contact between the substrate and the trypsin, speeding up the enzymatic reaction rate. This stabilizing effect mainly derived from the virtue of NADES 1-7 itself rather than the superposition of individual components. Additionally, spectral analysis revealed that the NADES 1-7 promoted trypsin conformational folding, effectively protecting the natural structure of trypsin. Importantly, the NADES 1-7 had good biocompatibility, further expanding its application." @default.
- W4381051388 created "2023-06-18" @default.
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- W4381051388 date "2023-08-01" @default.
- W4381051388 modified "2023-09-24" @default.
- W4381051388 title "Natural deep eutectic solvent—A novel green solvent for protein stabilization" @default.
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- W4381051388 doi "https://doi.org/10.1016/j.ijbiomac.2023.125477" @default.
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