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- W4381059319 abstract "Polymerase Chain Reaction (PCR) has revolutionized the field of molecular biology since its invention in 1983. The technique has enabled scientists to amplify specific sequences of the genomic DNA for various applications, from basic research to clinical diagnosis. However, like any other experimental protocol, PCR has its limitations and challenges. Troubleshooting and optimization are crucial steps for achieving consistent and reliable results in PCR. This review provides an overview of the history of PCR, highlighting its evolution over the past four decades. The article also discusses common troubleshooting strategies and optimization methods that researchers use to overcome obstacles in PCR experiments. By understanding the principles and techniques of PCR optimization, scientists can design experiments that efficiently amplify specific DNA sequences, minimize non-specific amplification, and improve the overall accuracy and sensitivity of the results." @default.
- W4381059319 created "2023-06-18" @default.
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- W4381059319 date "2020-01-10" @default.
- W4381059319 modified "2023-10-14" @default.
- W4381059319 title "Revolutionizing Molecular Biology: The Evolution of PCR through Troubleshooting and Optimization" @default.
- W4381059319 doi "https://doi.org/10.52228/nbw-jaab.2020-2-1-3" @default.
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