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- W4381082014 abstract "Abstract Multi-omics requires concerted recording of independent information, ideally from a single experiment. In this study, we introduce RIMS-seq2, a high-throughput technique to simultaneously sequence genomes and overlay methylation information while requiring only a small modification of the experimental protocol for high throughput DNA sequencing to include a controlled deamination step. Importantly, the rate of deamination of 5mC is negligible and thus, do not interfere with standard DNA sequencing and data processing. Thus, RIMS-seq2 libraries from whole or targeted genome sequencing show the same germline variation calling accuracy and sensitivity as compared to standard DNA-seq. Additionally, regional methylation levels provide an accurate map of the human methylome." @default.
- W4381082014 created "2023-06-18" @default.
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- W4381082014 date "2023-06-17" @default.
- W4381082014 modified "2023-10-03" @default.
- W4381082014 title "Simultaneous assessment of human genome and methylome data in a single experiment using limited deamination of methylated cytosine" @default.
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- W4381082014 doi "https://doi.org/10.1101/2023.06.16.545253" @default.
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