FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381331566> ?p ?o ?g. }

• W4381331566 endingPage "104668" @default.
• W4381331566 startingPage "104668" @default.
• W4381331566 abstract "Previous studies have shown a positive correlation between the length of estrus prior to ovulation and fertility in cyclic donors and embryo recipient mares. Furthermore, higher endometrial expression of Uterocalin (P19) was found in anestrous mares treated with a 5 day estradiol protocol, compared to recipient mares with a short or no estradiol priming. However, it is not known how this extended estradiol exposure influences embryo survival. The objective of this study was to determine the effect of different lengths of estradiol treatment on embryo survival, growth and proportion of mitotic cells. Three experimental groups of anestrous recipient mares were used: (1) mares treated with long-acting progesterone (LA P4) only (NE); (2) mares treated with a single administration of 2.5mg of estradiol benzoate (SE) 2 days before LA P4; and (3) mares treated with 8mg of estradiol split into increasing doses for 5 consecutive days (LE) ending 3 days before LA P4 treatment. Embryos were recovered from donor mares 8 days after ovulation, and embryo diameter and grade were recorded. Only grade I and II expanded blastocysts were used for this study; they were transferred into recipient mares using a modified Wilsher's technique 4 days after LA P4. Embryo recovery was performed 2 days after transfer by uterine lavage to examine embryo survival and growth rate inthe three experimental groups. After a positive recovery, embryos were evaluated for grade, size and fixed in 1% paraformaldehyde for subsequent staining with Hoechst 33342 for nuclear evaluation by confocal microscopy. The proportion of mitotic cells was assessed for 4 areas (10,000µm2) per embryo. The likelihood of recovering an embryo 2 days after transfer was 46.1% (6/13), 62.5% (5/8) and 85.7% (6/7) for recipient mares from the NE, SE and LE groups, respectively (p =.09). The embryos recovered from the three different groups of recipients 48h after transfer did not, however, show any differences in size, morphology or in the proportion of nuclei undergoing mitosis (p>0.05). In conclusion, the duration of estradiol treatment before progesterone administration tended to influence embryo survival in anestrous recipients within 2 days of transfer. However, the grade and growth rate of surviving embryos did not differ among groups. This may indicate that if the endometrial environment is not optimal to support embryo development following transfer, the embryo dies and disappears relatively quickly, i.e. within 48 h, whereas surviving embryos continue to develop normally." @default.
• W4381331566 created "2023-06-21" @default.
• W4381331566 creator A5004852994 @default.
• W4381331566 creator A5014367175 @default.
• W4381331566 creator A5020348928 @default.
• W4381331566 creator A5035703543 @default.
• W4381331566 creator A5036291087 @default.
• W4381331566 creator A5051742811 @default.
• W4381331566 creator A5071076200 @default.
• W4381331566 date "2023-06-01" @default.
• W4381331566 modified "2023-10-02" @default.
• W4381331566 title "Estradiol treatment prior to progesterone favors embryo survival in anestrous recipient mares" @default.
• W4381331566 doi "https://doi.org/10.1016/j.jevs.2023.104668" @default.
• W4381331566 hasPublicationYear "2023" @default.
• W4381331566 type Work @default.
• W4381331566 citedByCount "0" @default.
• W4381331566 crossrefType "journal-article" @default.
• W4381331566 hasAuthorship W4381331566A5004852994 @default.
• W4381331566 hasAuthorship W4381331566A5014367175 @default.
• W4381331566 hasAuthorship W4381331566A5020348928 @default.
• W4381331566 hasAuthorship W4381331566A5035703543 @default.
• W4381331566 hasAuthorship W4381331566A5036291087 @default.
• W4381331566 hasAuthorship W4381331566A5051742811 @default.
• W4381331566 hasAuthorship W4381331566A5071076200 @default.
• W4381331566 hasConcept C126322002 @default.
• W4381331566 hasConcept C134018914 @default.
• W4381331566 hasConcept C160099875 @default.
• W4381331566 hasConcept C16685009 @default.
• W4381331566 hasConcept C196843134 @default.
• W4381331566 hasConcept C1998276 @default.
• W4381331566 hasConcept C2777164284 @default.
• W4381331566 hasConcept C2777702977 @default.
• W4381331566 hasConcept C2778506572 @default.
• W4381331566 hasConcept C71315377 @default.
• W4381331566 hasConcept C71924100 @default.
• W4381331566 hasConcept C8264082 @default.
• W4381331566 hasConcept C86803240 @default.
• W4381331566 hasConcept C95444343 @default.
• W4381331566 hasConceptScore W4381331566C126322002 @default.
• W4381331566 hasConceptScore W4381331566C134018914 @default.
• W4381331566 hasConceptScore W4381331566C160099875 @default.
• W4381331566 hasConceptScore W4381331566C16685009 @default.
• W4381331566 hasConceptScore W4381331566C196843134 @default.
• W4381331566 hasConceptScore W4381331566C1998276 @default.
• W4381331566 hasConceptScore W4381331566C2777164284 @default.
• W4381331566 hasConceptScore W4381331566C2777702977 @default.
• W4381331566 hasConceptScore W4381331566C2778506572 @default.
• W4381331566 hasConceptScore W4381331566C71315377 @default.
• W4381331566 hasConceptScore W4381331566C71924100 @default.
• W4381331566 hasConceptScore W4381331566C8264082 @default.
• W4381331566 hasConceptScore W4381331566C86803240 @default.
• W4381331566 hasConceptScore W4381331566C95444343 @default.
• W4381331566 hasLocation W43813315661 @default.
• W4381331566 hasOpenAccess W4381331566 @default.
• W4381331566 hasPrimaryLocation W43813315661 @default.
• W4381331566 hasRelatedWork W1985546601 @default.
• W4381331566 hasRelatedWork W2040045886 @default.
• W4381331566 hasRelatedWork W2042574196 @default.
• W4381331566 hasRelatedWork W2054751732 @default.
• W4381331566 hasRelatedWork W2064155721 @default.
• W4381331566 hasRelatedWork W2071688816 @default.
• W4381331566 hasRelatedWork W2144224914 @default.
• W4381331566 hasRelatedWork W2156499301 @default.
• W4381331566 hasRelatedWork W2167902350 @default.
• W4381331566 hasRelatedWork W2169978493 @default.
• W4381331566 hasVolume "125" @default.
• W4381331566 isParatext "false" @default.
• W4381331566 isRetracted "false" @default.
• W4381331566 workType "article" @default.