FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381332572> ?p ?o ?g. }

• W4381332572 endingPage "104591" @default.
• W4381332572 startingPage "104591" @default.
• W4381332572 abstract "Thoroughbred (TB) stallions that carry the linked susceptibility genotype A/A-A/A in exon 5 of the FKBP6 gene (ECA13; EquCab 3.0) are profoundly subfertile due to Impaired Acrosomal Exocytosis (IAE). A clear causation of the FKBP6 genotype and IAE is still unknown. In the current study, the sperm proteome in frozen/thawed semen from three fertile TB stallions (n = 3) and three subfertile TB stallions (FKBP6 genotype A/A-A/A; n = 3) was studied using mass spectrometry. Sperm from both stallion groups were incubated in Modified Whitten's-lactate media (MW-Lac) to induce acrosomal exocytosis in viable sperm (AE/Viable). At 0h, 2h, 4h, and 6h, sperm aliquots were analyzed for AE/Viable using flow cytometry (Fixable Live/Dead Red + FITC-PSA), while the sperm proteome was analyzed via Data-Independent Acquisition mass spectrometry (DIA-MS). Student's t-tests and two-way ANOVA with Benjamini-Hochberg multiple testing correction (FDR q-value 0.05) were used to determine differences in AE/Viable and protein relative abundance between experimental groups and incubation periods. At 4h and 6h of incubation, the mean AE/Viable was higher in the fertile than in the subfertile stallions (41 and 44% vs. 14 and 16%, respectively; P < 0.05). A total of 2220 proteins were identified by DIA-MS. Usingstrict selection criteria (FDR 1.0%, q-value < 0.05, and fold-change < 1.5 or > 1.5), 140 proteins were found to be differentially abundant in sperm from the subfertile stallions when compared to that of the fertile stallions (83 less and 57 more abundant). Using bioinformatic analyses, most of the proteins of lower abundance in sperm from subfertile stallions were found to be overrepresented in the “metabolism” (32 proteins) and “metabolism of lipids” (18 proteins) pathways (Homo sapiens orthologs; Reactome database). Two of these proteins, arylsulfatase F (ARSF; fold-change < 4.02; P < 0.05) and zona pellucida-binding protein 2 (ZPBP2; fold-change < 3.36; P < 0.05), are acrosomal proteins known to play a fundamental role in sperm-oocyte binding. By using immunofluorescence, at 0h of incubation in MW-Lac, ARSF was identified at the acrosome, mid-, and principal piece locations in sperm from fertile TB stallions, while only at the mid- and principal piece regions in sperm from subfertile TB stallions. No evidence of ZPBP2 was observed in sperm from both stallion groups at 0h incubation in Lac-MW. Current studies are focused on determining whether these proteins undergo changes in localization within the sperm during acrosomal exocytosis." @default.
• W4381332572 created "2023-06-21" @default.
• W4381332572 creator A5005466573 @default.
• W4381332572 creator A5029979371 @default.
• W4381332572 creator A5049175374 @default.
• W4381332572 creator A5073427041 @default.
• W4381332572 creator A5084985072 @default.
• W4381332572 date "2023-06-01" @default.
• W4381332572 modified "2023-09-27" @default.
• W4381332572 title "The acrosome proteins arylsulfatase F and zona pellucida-binding protein 2 are candidate markers associated with Impaired Acrosomal Exocytosis in sperm from subfertile Thoroughbred stallions" @default.
• W4381332572 doi "https://doi.org/10.1016/j.jevs.2023.104591" @default.
• W4381332572 hasPublicationYear "2023" @default.
• W4381332572 type Work @default.
• W4381332572 citedByCount "0" @default.
• W4381332572 crossrefType "journal-article" @default.
• W4381332572 hasAuthorship W4381332572A5005466573 @default.
• W4381332572 hasAuthorship W4381332572A5029979371 @default.
• W4381332572 hasAuthorship W4381332572A5049175374 @default.
• W4381332572 hasAuthorship W4381332572A5073427041 @default.
• W4381332572 hasAuthorship W4381332572A5084985072 @default.
• W4381332572 hasConcept C134018914 @default.
• W4381332572 hasConcept C16685009 @default.
• W4381332572 hasConcept C175969161 @default.
• W4381332572 hasConcept C203014093 @default.
• W4381332572 hasConcept C25642318 @default.
• W4381332572 hasConcept C2777589648 @default.
• W4381332572 hasConcept C2777688143 @default.
• W4381332572 hasConcept C2778093475 @default.
• W4381332572 hasConcept C2778252442 @default.
• W4381332572 hasConcept C2779234561 @default.
• W4381332572 hasConcept C2781087480 @default.
• W4381332572 hasConcept C49039625 @default.
• W4381332572 hasConcept C54355233 @default.
• W4381332572 hasConcept C553184892 @default.
• W4381332572 hasConcept C55493867 @default.
• W4381332572 hasConcept C71924100 @default.
• W4381332572 hasConcept C86803240 @default.
• W4381332572 hasConceptScore W4381332572C134018914 @default.
• W4381332572 hasConceptScore W4381332572C16685009 @default.
• W4381332572 hasConceptScore W4381332572C175969161 @default.
• W4381332572 hasConceptScore W4381332572C203014093 @default.
• W4381332572 hasConceptScore W4381332572C25642318 @default.
• W4381332572 hasConceptScore W4381332572C2777589648 @default.
• W4381332572 hasConceptScore W4381332572C2777688143 @default.
• W4381332572 hasConceptScore W4381332572C2778093475 @default.
• W4381332572 hasConceptScore W4381332572C2778252442 @default.
• W4381332572 hasConceptScore W4381332572C2779234561 @default.
• W4381332572 hasConceptScore W4381332572C2781087480 @default.
• W4381332572 hasConceptScore W4381332572C49039625 @default.
• W4381332572 hasConceptScore W4381332572C54355233 @default.
• W4381332572 hasConceptScore W4381332572C553184892 @default.
• W4381332572 hasConceptScore W4381332572C55493867 @default.
• W4381332572 hasConceptScore W4381332572C71924100 @default.
• W4381332572 hasConceptScore W4381332572C86803240 @default.
• W4381332572 hasLocation W43813325721 @default.
• W4381332572 hasOpenAccess W4381332572 @default.
• W4381332572 hasPrimaryLocation W43813325721 @default.
• W4381332572 hasRelatedWork W1843461771 @default.
• W4381332572 hasRelatedWork W1965693865 @default.
• W4381332572 hasRelatedWork W2040132996 @default.
• W4381332572 hasRelatedWork W2107885773 @default.
• W4381332572 hasRelatedWork W2117254142 @default.
• W4381332572 hasRelatedWork W2146810853 @default.
• W4381332572 hasRelatedWork W2359019635 @default.
• W4381332572 hasRelatedWork W2370897094 @default.
• W4381332572 hasRelatedWork W4283722110 @default.
• W4381332572 hasRelatedWork W4382767079 @default.
• W4381332572 hasVolume "125" @default.
• W4381332572 isParatext "false" @default.
• W4381332572 isRetracted "false" @default.
• W4381332572 workType "article" @default.