Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381377368> ?p ?o ?g. }
Showing items 1 to 50 of
50
with 100 items per page.
- W4381377368 abstract "The beta cell transcription factor Nkx6.1 is critical in pancreatic progenitor cells and mature beta cells. The pathways regulated by Nkx6.1 that results in these phenotypes are still being defined. We sought to define the transcriptional factors that interact with Nkx6.1 and may be harnessed to expand functional beta cell mass. INS-1 832/13 beta cells were transduced with AdCMV-Nkx6.1-BioID, AdCMV-GFP-BioID or left untreated. Biotinylated proteins were isolated and the Nkx6.1 interactome was defined using liquid chromatography-tandem mass spectrometry. We defined 176 biotinylated proteins in cells transduced with AdCMV-Nkx6.1-BioID that were not observed with AdCMV-GFP-BioID or naturally biotinylated proteins from untreated lysates. These potential Nkx6.1 interacting proteins are enriched in nucleotide binding, ribosomal proteins, and transcription factors. Among the enriched transcription factors, we validated that Pdx1 is biotinylated in response to transduction with AdCMV-Nkx6.1 using biotin-targeted immunoprecipitation and western blot. To examine if Pdx1 is likely to interact with Nkx6.1 on the genome, we analyzed the frequency of proximal binding sites for Nkx6.1 and Pdx1, and demonstrated that Pdx1 and Nkx6.1 binding sites in close proximity were statistically overrepresented in the genome. Given the role of Pdx1 on functional beta cell mass, we further characterized the interaction of endogenous Nkx6.1 and Pdx1 by co-immunoprecipitation (Co-IP). Our data demonstrates significant Pdx1 binding by Nkx6.1 Co-IP and significant Nkx6.1 binding by Pdx1 Co-IP, as compared to Co-IP with the IgG control. Finally, we demonstrate coregulation of Unc3 expression by Nkx6.1 and Pdx1 and demonstrate that CRISPR mediated deletion of either Nkx6.1 or Pdx1 is sufficient to impair INS-1 832/13 mediated expression of Unc3. These data define potential Nkx6.1 binding partners in INS-1 832/13 cells, as well as novel interaction between Nkx6.1 and Pdx1 in the regulation of Unc3. Disclosure J.S.Tessem: None. C.C.Littlefield: None. J.T.Hill: None." @default.
- W4381377368 created "2023-06-21" @default.
- W4381377368 creator A5023893268 @default.
- W4381377368 creator A5065367138 @default.
- W4381377368 creator A5084242179 @default.
- W4381377368 date "2023-06-20" @default.
- W4381377368 modified "2023-09-27" @default.
- W4381377368 title "1737-P: Novel Interaction between Nkx6.1 and Pdx1 Is Needed for Proper Beta-Cell Function" @default.
- W4381377368 doi "https://doi.org/10.2337/db23-1737-p" @default.
- W4381377368 hasPublicationYear "2023" @default.
- W4381377368 type Work @default.
- W4381377368 citedByCount "0" @default.
- W4381377368 crossrefType "journal-article" @default.
- W4381377368 hasAuthorship W4381377368A5023893268 @default.
- W4381377368 hasAuthorship W4381377368A5065367138 @default.
- W4381377368 hasAuthorship W4381377368A5084242179 @default.
- W4381377368 hasConcept C104317684 @default.
- W4381377368 hasConcept C153911025 @default.
- W4381377368 hasConcept C204628709 @default.
- W4381377368 hasConcept C2779050171 @default.
- W4381377368 hasConcept C54355233 @default.
- W4381377368 hasConcept C86339819 @default.
- W4381377368 hasConcept C86803240 @default.
- W4381377368 hasConcept C95444343 @default.
- W4381377368 hasConceptScore W4381377368C104317684 @default.
- W4381377368 hasConceptScore W4381377368C153911025 @default.
- W4381377368 hasConceptScore W4381377368C204628709 @default.
- W4381377368 hasConceptScore W4381377368C2779050171 @default.
- W4381377368 hasConceptScore W4381377368C54355233 @default.
- W4381377368 hasConceptScore W4381377368C86339819 @default.
- W4381377368 hasConceptScore W4381377368C86803240 @default.
- W4381377368 hasConceptScore W4381377368C95444343 @default.
- W4381377368 hasIssue "Supplement_1" @default.
- W4381377368 hasLocation W43813773681 @default.
- W4381377368 hasOpenAccess W4381377368 @default.
- W4381377368 hasPrimaryLocation W43813773681 @default.
- W4381377368 hasRelatedWork W2009710060 @default.
- W4381377368 hasRelatedWork W2012231968 @default.
- W4381377368 hasRelatedWork W2024682955 @default.
- W4381377368 hasRelatedWork W2035090740 @default.
- W4381377368 hasRelatedWork W2057841153 @default.
- W4381377368 hasRelatedWork W2065034164 @default.
- W4381377368 hasRelatedWork W2067754307 @default.
- W4381377368 hasRelatedWork W2462922943 @default.
- W4381377368 hasRelatedWork W4224281065 @default.
- W4381377368 hasRelatedWork W4232976280 @default.
- W4381377368 hasVolume "72" @default.
- W4381377368 isParatext "false" @default.
- W4381377368 isRetracted "false" @default.
- W4381377368 workType "article" @default.