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- W4381384311 abstract "Type IV CRISPR-Cas systems, which are primarily found on plasmids and exhibit a strong plasmid-targeting preference, are the only one of the six known CRISPR-Cas types for which the mechanistic details of their function remain unknown. Here, we provide high-resolution functional snapshots of type IV-A Csf complexes before and after target dsDNA binding, either in the absence or presence of CasDinG, revealing the mechanisms underlying CsfcrRNA complex assembly, DWN PAM-dependent dsDNA targeting, R-loop formation, and CasDinG recruitment. Furthermore, we establish that CasDinG, a signature DinG family helicase, harbors ssDNA-stimulated ATPase activity and ATP-dependent 5'-3' DNA helicase activity. In addition, we show that CasDinG unwinds the non-target strand (NTS) and target strand (TS) of target dsDNA from the CsfcrRNA complex. These molecular details advance our mechanistic understanding of type IV-A CRISPR-Csf function and should enable Csf complexes to be harnessed as genome-engineering tools for biotechnological applications." @default.
- W4381384311 created "2023-06-21" @default.
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- W4381384311 date "2023-07-01" @default.
- W4381384311 modified "2023-10-05" @default.
- W4381384311 title "Type IV-A CRISPR-Csf complex: Assembly, dsDNA targeting, and CasDinG recruitment" @default.
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- W4381384311 doi "https://doi.org/10.1016/j.molcel.2023.05.036" @default.
- W4381384311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37343553" @default.
- W4381384311 hasPublicationYear "2023" @default.
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