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- W4381434019 endingPage "e1010819" @default.
- W4381434019 startingPage "e1010819" @default.
- W4381434019 abstract "C-di-GMP is a bacterial second messenger that regulates diverse processes in response to environmental or cellular cues. The nucleoid-associated protein (NAP) CdbA in Myxococcus xanthus binds c-di-GMP and DNA in a mutually exclusive manner in vitro. CdbA is essential for viability, and CdbA depletion causes defects in chromosome organization, leading to a block in cell division and, ultimately, cell death. Most NAPs are not essential; therefore, to explore the paradoxical cdbA essentiality, we isolated suppressor mutations that restored cell viability without CdbA. Most mutations mapped to cdbS, which encodes a stand-alone c-di-GMP binding PilZ domain protein, and caused loss-of-function of cdbS. Cells lacking CdbA and CdbS or only CdbS were fully viable and had no defects in chromosome organization. CdbA depletion caused post-transcriptional upregulation of CdbS accumulation, and this CdbS over-accumulation was sufficient to disrupt chromosome organization and cause cell death. CdbA depletion also caused increased accumulation of CsdK1 and CsdK2, two unusual PilZ-DnaK chaperones. During CdbA depletion, CsdK1 and CsdK2, in turn, enabled the increased accumulation and toxicity of CdbS, likely by stabilizing CdbS. Moreover, we demonstrate that heat stress, possibly involving an increased cellular c-di-GMP concentration, induced the CdbA/CsdK1/CsdK2/CdbS system, causing a CsdK1- and CsdK2-dependent increase in CdbS accumulation. Thereby this system accelerates heat stress-induced chromosome mis-organization and cell death. Collectively, this work describes a unique system that contributes to regulated cell death in M. xanthus and suggests a link between c-di-GMP signaling and regulated cell death in bacteria." @default.
- W4381434019 created "2023-06-21" @default.
- W4381434019 creator A5019301672 @default.
- W4381434019 creator A5048083609 @default.
- W4381434019 creator A5063694214 @default.
- W4381434019 creator A5074705819 @default.
- W4381434019 date "2023-06-20" @default.
- W4381434019 modified "2023-10-05" @default.
- W4381434019 title "During heat stress in Myxococcus xanthus, the CdbS PilZ domain protein, in concert with two PilZ-DnaK chaperones, perturbs chromosome organization and accelerates cell death" @default.
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- W4381434019 doi "https://doi.org/10.1371/journal.pgen.1010819" @default.
- W4381434019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37339150" @default.
- W4381434019 hasPublicationYear "2023" @default.
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