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• W4381434350 abstract "Abstract Reproductive traits play a vital role in determining the production efficiency of sheep. Maximizing the production is of paramount importance for breeders worldwide due to the growing population. Circular RNAs (circRNAs) act as miRNA sponges by absorbing miRNA activity through miRNA response elements (MREs) and participate in ceRNA regulatory networks (ceRNETs) to regulate mRNA expression. Despite of extensive research on role of circRNAs as miRNA sponges in various species, their specific regulatory roles and mechanism in sheep ovarian tissue are still not well understood. In this study, we performed whole genome sequencing of circRNAs, miRNA and mRNA employing bioinformatic techniques on ovine tissues of two contrasting sheep breeds Small tail Han (X_LC) and Dolang sheep (D_LC), which results into identification of 9,878 circRNAs with a total length of 23,522,667 nt and an average length of 2,381.32 nt. Among them, 44 differentially expressed circRNAs (DECs) were identified. Moreover, correlation between miRNA-mRNA and lncRNA-miRNA provided us with to prediction of miRNA binding sites on nine differentially expressed circRNAs and 165 differentially expressed mRNAs using miRanda. miRNA-mRNA and lncRNA-miRNA pairs with negative correlation were selected to determine the ceRNA score along with positively correlated pairs from lncRNA and mRNA network. Integration of ceRNA score and positively correlated pairs exhibit a significant ternary relationship among circRNAs-miRNA-mRNA demonestrated by ceRNA, comprising of 50 regulatory pairs sharring common nodes and predicted potential differentially expressed circRNAs-miRNAs-mRNAs regulatory axis. Based on functional enrichment analysis shortlisted key ceRNA regulatory pairs associated with reproduction including circRNA_3257-novel579_mature-EPHA3, circRNA_8396-novel130_mature-LOC101102473, circRNA_4140- novel34_mature > novel661_mature-KCNK9, and circRNA_8312-novel339_mature-LOC101110545. Furthermore, expression profiling, functional enrichments and qRT-PCR analysis of key target genes infer their implication in reproduction and metabolism. ceRNA target mRNAs evolutionary trajectories, expression profiling, functional enrichments, subcellular localizations following genomic organizations will provide new insights underlying molecular mechanisms of reproduction, and establish a solid foundation for future research. Graphical Abstract Graphical abstract summarizing the scheme of study" @default.
• W4381434350 created "2023-06-21" @default.
• W4381434350 creator A5020549462 @default.
• W4381434350 creator A5029817092 @default.
• W4381434350 creator A5070085914 @default.
• W4381434350 creator A5070818316 @default.
• W4381434350 creator A5078076409 @default.
• W4381434350 creator A5089216927 @default.
• W4381434350 date "2023-06-20" @default.
• W4381434350 modified "2023-10-18" @default.
• W4381434350 title "Genome wide identification and characterization of fertility associated novel CircRNAs as ceRNA reveal their regulatory roles in sheep fecundity" @default.
• W4381434350 cites W1507082667 @default.
• W4381434350 cites W1549438987 @default.
• W4381434350 cites W1658681964 @default.
• W4381434350 cites W1846631206 @default.
• W4381434350 cites W1966411071 @default.
• W4381434350 cites W1971654640 @default.
• W4381434350 cites W1978872463 @default.
• W4381434350 cites W1980196019 @default.
• W4381434350 cites W1987841836 @default.
• W4381434350 cites W1995776632 @default.
• W4381434350 cites W1997708855 @default.
• W4381434350 cites W2004714113 @default.
• W4381434350 cites W2006571532 @default.
• W4381434350 cites W2009881536 @default.
• W4381434350 cites W2013198813 @default.
• W4381434350 cites W2013937380 @default.
• W4381434350 cites W2015272656 @default.
• W4381434350 cites W2015296829 @default.
• W4381434350 cites W2032940703 @default.
• W4381434350 cites W2036402097 @default.
• W4381434350 cites W2036899354 @default.
• W4381434350 cites W2037098597 @default.
• W4381434350 cites W2038191526 @default.
• W4381434350 cites W2040898123 @default.
• W4381434350 cites W2046387002 @default.
• W4381434350 cites W2057064377 @default.
• W4381434350 cites W2090419112 @default.
• W4381434350 cites W2091494076 @default.
• W4381434350 cites W2092723228 @default.
• W4381434350 cites W2096021370 @default.
• W4381434350 cites W2110048796 @default.
• W4381434350 cites W2113239055 @default.
• W4381434350 cites W2120456037 @default.
• W4381434350 cites W2127578454 @default.
• W4381434350 cites W2129721415 @default.
• W4381434350 cites W2131865548 @default.
• W4381434350 cites W2133150565 @default.
• W4381434350 cites W2135398049 @default.
• W4381434350 cites W2145301393 @default.
• W4381434350 cites W2147625377 @default.
• W4381434350 cites W2149176074 @default.
• W4381434350 cites W2152826678 @default.
• W4381434350 cites W2161608856 @default.
• W4381434350 cites W2168002090 @default.
• W4381434350 cites W2179438025 @default.
• W4381434350 cites W2269488904 @default.
• W4381434350 cites W2285247593 @default.
• W4381434350 cites W2311203695 @default.
• W4381434350 cites W2409945447 @default.
• W4381434350 cites W2414945950 @default.
• W4381434350 cites W2417694506 @default.
• W4381434350 cites W2510284858 @default.
• W4381434350 cites W2510302715 @default.
• W4381434350 cites W2521514890 @default.
• W4381434350 cites W2530955502 @default.
• W4381434350 cites W2549560046 @default.
• W4381434350 cites W2553510051 @default.
• W4381434350 cites W2566373663 @default.
• W4381434350 cites W2580767461 @default.
• W4381434350 cites W2599335862 @default.
• W4381434350 cites W2743261589 @default.
• W4381434350 cites W2766800766 @default.
• W4381434350 cites W2767085919 @default.
• W4381434350 cites W2770495047 @default.
• W4381434350 cites W2774878892 @default.
• W4381434350 cites W2776471838 @default.
• W4381434350 cites W2790630451 @default.
• W4381434350 cites W2793761502 @default.
• W4381434350 cites W2797047083 @default.
• W4381434350 cites W2803062158 @default.
• W4381434350 cites W2805961026 @default.
• W4381434350 cites W2810951976 @default.
• W4381434350 cites W2883303543 @default.
• W4381434350 cites W2889250831 @default.
• W4381434350 cites W2891057708 @default.
• W4381434350 cites W2894408179 @default.
• W4381434350 cites W2898638081 @default.
• W4381434350 cites W2903464283 @default.
• W4381434350 cites W2913701343 @default.
• W4381434350 cites W2941873727 @default.
• W4381434350 cites W2943210965 @default.
• W4381434350 cites W2944605127 @default.
• W4381434350 cites W2954501229 @default.
• W4381434350 cites W2965472106 @default.
• W4381434350 cites W2968045890 @default.
• W4381434350 cites W2980236246 @default.
• W4381434350 cites W2990999726 @default.
• W4381434350 cites W2999533766 @default.
• W4381434350 cites W3000395304 @default.

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