FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381469830> ?p ?o ?g. }

• W4381469830 abstract "ABSTRACT Toxoplasma gondii is an obligate, intracellular parasite. Infection of a cell produces a unique niche for the parasite named the parasitophorous vacuole (PV) initially composed of host plasma membrane invaginated during invasion. The PV and its membrane (parasitophorous vacuole membrane [PVM]) are subsequently decorated with a variety of parasite proteins allowing the parasite to optimally grow in addition to manipulate host processes. Recently, we reported a proximity-labeling screen at the PVM–host interface and identified host endoplasmic reticulum (ER)-resident motile sperm domain-containing protein 2 (MOSPD2) as being enriched at this location. Here we extend these findings in several important respects. First, we show that the extent and pattern of host MOSPD2 association with the PVM differ dramatically in cells infected with different strains of Toxoplasma . Second, in cells infected with Type I RH strain, the MOSPD2 staining is mutually exclusive with regions of the PVM that associate with mitochondria. Third, immunoprecipitation and liquid chromatography tandem mass spectrometry (LC-MS/MS) with epitope-tagged MOSPD2-expressing host cells reveal strong enrichment of several PVM-localized parasite proteins, although none appear to play an essential role in MOSPD2 association. Fourth, most MOSPD2 associating with the PVM is newly translated after infection of the cell and requires the major functional domains of MOSPD2, identified as the CRAL/TRIO domain and tail anchor, although these domains were not sufficient for PVM association. Lastly, ablation of MOSPD2 results in, at most, a modest impact on Toxoplasma growth in vitro . Collectively, these studies provide new insight into the molecular interactions involving MOSPD2 at the dynamic interface between the PVM and the host cytosol. IMPORTANCE Toxoplasma gondii is an intracellular pathogen that lives within a membranous vacuole inside of its host cell. This vacuole is decorated by a variety of parasite proteins that allow it to defend against host attack, acquire nutrients, and interact with the host cell. Recent work identified and validated host proteins enriched at this host–pathogen interface. Here, we follow up on one candidate named MOSPD2 shown to be enriched at the vacuolar membrane and describe it as having a dynamic interaction at this location depending on a variety of factors. Some of these include the presence of host mitochondria, intrinsic domains of the host protein, and whether translation is active. Importantly, we show that MOSPD2 enrichment at the vacuole membrane differs between strains indicating active involvement of the parasite with this phenotype. Altogether, these results shed light on the mechanism and role of protein associations in the host–pathogen interaction." @default.
• W4381469830 created "2023-06-22" @default.
• W4381469830 creator A5053925806 @default.
• W4381469830 creator A5059796157 @default.
• W4381469830 creator A5083405606 @default.
• W4381469830 creator A5084166797 @default.
• W4381469830 creator A5087130929 @default.
• W4381469830 date "2023-08-24" @default.
• W4381469830 modified "2023-09-25" @default.
• W4381469830 title "Host MOSPD2 enrichment at the parasitophorous vacuole membrane varies between <i>Toxoplasma</i> strains and involves complex interactions" @default.
• W4381469830 cites W116368854 @default.
• W4381469830 cites W1580003617 @default.
• W4381469830 cites W160386431 @default.
• W4381469830 cites W1747545477 @default.
• W4381469830 cites W1823778824 @default.
• W4381469830 cites W1952642745 @default.
• W4381469830 cites W1955764924 @default.
• W4381469830 cites W1964706690 @default.
• W4381469830 cites W1966184958 @default.
• W4381469830 cites W1969325783 @default.
• W4381469830 cites W1970998121 @default.
• W4381469830 cites W1974483800 @default.
• W4381469830 cites W1976781467 @default.
• W4381469830 cites W1978466652 @default.
• W4381469830 cites W1979300255 @default.
• W4381469830 cites W1993484262 @default.
• W4381469830 cites W1998615549 @default.
• W4381469830 cites W2007833275 @default.
• W4381469830 cites W2014645398 @default.
• W4381469830 cites W2015293161 @default.
• W4381469830 cites W2016336737 @default.
• W4381469830 cites W2019946319 @default.
• W4381469830 cites W2028293593 @default.
• W4381469830 cites W2034870393 @default.
• W4381469830 cites W2039242962 @default.
• W4381469830 cites W2045581926 @default.
• W4381469830 cites W2049144892 @default.
• W4381469830 cites W2058172718 @default.
• W4381469830 cites W2058372689 @default.
• W4381469830 cites W2071990248 @default.
• W4381469830 cites W2072773040 @default.
• W4381469830 cites W2074354155 @default.
• W4381469830 cites W2075233599 @default.
• W4381469830 cites W2083692164 @default.
• W4381469830 cites W2100075651 @default.
• W4381469830 cites W2103059292 @default.
• W4381469830 cites W2109302611 @default.
• W4381469830 cites W2112444305 @default.
• W4381469830 cites W2116537957 @default.
• W4381469830 cites W2117235669 @default.
• W4381469830 cites W2118954525 @default.
• W4381469830 cites W2139042645 @default.
• W4381469830 cites W2144924711 @default.
• W4381469830 cites W2145663614 @default.
• W4381469830 cites W2147828217 @default.
• W4381469830 cites W2156273957 @default.
• W4381469830 cites W2163174085 @default.
• W4381469830 cites W2164275380 @default.
• W4381469830 cites W2271466588 @default.
• W4381469830 cites W2285870394 @default.
• W4381469830 cites W2342979405 @default.
• W4381469830 cites W2562986297 @default.
• W4381469830 cites W2606736166 @default.
• W4381469830 cites W2616561032 @default.
• W4381469830 cites W2618451207 @default.
• W4381469830 cites W2747992208 @default.
• W4381469830 cites W2766449056 @default.
• W4381469830 cites W2767586726 @default.
• W4381469830 cites W2782042989 @default.
• W4381469830 cites W2785175268 @default.
• W4381469830 cites W2799855588 @default.
• W4381469830 cites W2806366302 @default.
• W4381469830 cites W2904226822 @default.
• W4381469830 cites W2952907948 @default.
• W4381469830 cites W2964294801 @default.
• W4381469830 cites W2964745820 @default.
• W4381469830 cites W2982701700 @default.
• W4381469830 cites W2999290570 @default.
• W4381469830 cites W3008898314 @default.
• W4381469830 cites W3022161384 @default.
• W4381469830 cites W3042279029 @default.
• W4381469830 cites W3093371308 @default.
• W4381469830 cites W3093474363 @default.
• W4381469830 cites W3114400592 @default.
• W4381469830 cites W3120071910 @default.
• W4381469830 cites W3177828909 @default.
• W4381469830 cites W3179146692 @default.
• W4381469830 cites W3179894177 @default.
• W4381469830 cites W3206744018 @default.
• W4381469830 cites W3208691473 @default.
• W4381469830 cites W3211795435 @default.
• W4381469830 cites W4200024783 @default.
• W4381469830 cites W4224073523 @default.
• W4381469830 cites W4226026871 @default.
• W4381469830 cites W4283265475 @default.
• W4381469830 cites W4302071594 @default.
• W4381469830 cites W4307659111 @default.
• W4381469830 doi "https://doi.org/10.1128/msphere.00670-22" @default.
• W4381469830 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37341482" @default.
• W4381469830 hasPublicationYear "2023" @default.

• next