FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381611478> ?p ?o ?g. }

• W4381611478 abstract "Triple‑negative breast cancer (TNBC) is an aggressive subtype of BC characterized by extensive intratumoral heterogeneity. Compared with other types of BC, TNBC is more prone to invasion and metastasis. The aim of the present study was to determine whether adenovirus‑mediated clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas9 system is capable of effectively targeting enhancer of zeste homolog 2 (EZH2) in TNBC cells and lay an experimental basis for the investigation of the CRISPR/Cas9 system as a gene therapy for BC. In the present study, EZH2 was knocked out in MDA‑MB‑231 cells using the CRISPR/Cas9 gene editing tool to create EZH2‑knockout (KO) group (EZH2‑KO group). Moreover, the GFP knockout group (control group), and a blank group (Blank group), were employed. The success of vector construction and EZH2‑KO were verified by T7 endonuclease I (T7EI) restriction enzyme digestion, mRNA detection and western blotting. Changes in proliferation and migration ability of MDA‑MB‑231 cells following gene editing were detected by MTT, wound healing, Transwell and in vivo tumor biology assays. As indicated by the results of mRNA and protein detection, the mRNA and protein expression of EZH2 were significantly downregulated in the EZH2‑KO group. The difference in EZH2 mRNA and protein between the EZH2‑KO and the two control groups was statistically significant. MTT, wound healing and transwell assay suggested that the proliferation and migration ability of MDA‑MB‑231 cells in the EZH2‑KO group were significantly decreased after EZH2 knockout. In vivo, the tumor growth rate in the EZH2‑KO group was significantly lower than that in the control groups. In brief, the present study revealed that the biological functions of tumor cells were inhibited after EZH2 knockout in MDA‑MB‑231 cells. The aforementioned findings suggested that EZH2 can have a key role in the development of TNBC." @default.
• W4381611478 created "2023-06-23" @default.
• W4381611478 creator A5029764346 @default.
• W4381611478 creator A5033581983 @default.
• W4381611478 creator A5051019409 @default.
• W4381611478 creator A5067940438 @default.
• W4381611478 creator A5069781169 @default.
• W4381611478 creator A5089146826 @default.
• W4381611478 date "2023-06-22" @default.
• W4381611478 modified "2023-10-18" @default.
• W4381611478 title "CRISPR/Cas9‑mediated EZH2 knockout suppresses the proliferation and migration of triple‑negative breast cancer cells" @default.
• W4381611478 cites W1571411512 @default.
• W4381611478 cites W1980009033 @default.
• W4381611478 cites W1986171241 @default.
• W4381611478 cites W1996487270 @default.
• W4381611478 cites W2018393962 @default.
• W4381611478 cites W2031190145 @default.
• W4381611478 cites W2050480410 @default.
• W4381611478 cites W2064815984 @default.
• W4381611478 cites W2077650275 @default.
• W4381611478 cites W2107277218 @default.
• W4381611478 cites W2118746294 @default.
• W4381611478 cites W2125496315 @default.
• W4381611478 cites W2155908077 @default.
• W4381611478 cites W2166139447 @default.
• W4381611478 cites W2170039745 @default.
• W4381611478 cites W2192080449 @default.
• W4381611478 cites W2256756668 @default.
• W4381611478 cites W2402636472 @default.
• W4381611478 cites W2560450396 @default.
• W4381611478 cites W2613712678 @default.
• W4381611478 cites W2741463184 @default.
• W4381611478 cites W2770685832 @default.
• W4381611478 cites W2783501849 @default.
• W4381611478 cites W2888645759 @default.
• W4381611478 cites W2889469543 @default.
• W4381611478 cites W2967721831 @default.
• W4381611478 cites W2972910291 @default.
• W4381611478 cites W2977305236 @default.
• W4381611478 cites W2999417355 @default.
• W4381611478 cites W3004591611 @default.
• W4381611478 cites W3009963727 @default.
• W4381611478 cites W3021361323 @default.
• W4381611478 cites W3092085090 @default.
• W4381611478 cites W3097446906 @default.
• W4381611478 cites W3100197573 @default.
• W4381611478 cites W3106937398 @default.
• W4381611478 cites W4200262030 @default.
• W4381611478 cites W4200419919 @default.
• W4381611478 cites W4200569802 @default.
• W4381611478 cites W4207033714 @default.
• W4381611478 cites W4213070361 @default.
• W4381611478 cites W4281710895 @default.
• W4381611478 cites W4283377778 @default.
• W4381611478 cites W4310121365 @default.
• W4381611478 cites W80971072 @default.
• W4381611478 doi "https://doi.org/10.3892/ol.2023.13929" @default.
• W4381611478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37427349" @default.
• W4381611478 hasPublicationYear "2023" @default.
• W4381611478 type Work @default.
• W4381611478 citedByCount "0" @default.
• W4381611478 crossrefType "journal-article" @default.
• W4381611478 hasAuthorship W4381611478A5029764346 @default.
• W4381611478 hasAuthorship W4381611478A5033581983 @default.
• W4381611478 hasAuthorship W4381611478A5051019409 @default.
• W4381611478 hasAuthorship W4381611478A5067940438 @default.
• W4381611478 hasAuthorship W4381611478A5069781169 @default.
• W4381611478 hasAuthorship W4381611478A5089146826 @default.
• W4381611478 hasBestOaLocation W43816114781 @default.
• W4381611478 hasConcept C104317684 @default.
• W4381611478 hasConcept C121608353 @default.
• W4381611478 hasConcept C150194340 @default.
• W4381611478 hasConcept C153911025 @default.
• W4381611478 hasConcept C2780110267 @default.
• W4381611478 hasConcept C50171091 @default.
• W4381611478 hasConcept C502942594 @default.
• W4381611478 hasConcept C530470458 @default.
• W4381611478 hasConcept C54355233 @default.
• W4381611478 hasConcept C86803240 @default.
• W4381611478 hasConcept C98108389 @default.
• W4381611478 hasConceptScore W4381611478C104317684 @default.
• W4381611478 hasConceptScore W4381611478C121608353 @default.
• W4381611478 hasConceptScore W4381611478C150194340 @default.
• W4381611478 hasConceptScore W4381611478C153911025 @default.
• W4381611478 hasConceptScore W4381611478C2780110267 @default.
• W4381611478 hasConceptScore W4381611478C50171091 @default.
• W4381611478 hasConceptScore W4381611478C502942594 @default.
• W4381611478 hasConceptScore W4381611478C530470458 @default.
• W4381611478 hasConceptScore W4381611478C54355233 @default.
• W4381611478 hasConceptScore W4381611478C86803240 @default.
• W4381611478 hasConceptScore W4381611478C98108389 @default.
• W4381611478 hasIssue "2" @default.
• W4381611478 hasLocation W43816114781 @default.
• W4381611478 hasLocation W43816114782 @default.
• W4381611478 hasLocation W43816114783 @default.
• W4381611478 hasOpenAccess W4381611478 @default.
• W4381611478 hasPrimaryLocation W43816114781 @default.
• W4381611478 hasRelatedWork W1176093231 @default.
• W4381611478 hasRelatedWork W1470355952 @default.
• W4381611478 hasRelatedWork W2754139274 @default.

• next