FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381619877> ?p ?o ?g. }

• W4381619877 abstract "Abstract Study question Does the duration of the interval between pronuclear fading and first cell division affect preimplantation embryo developmental competence? Summary answer Larger pronuclear-fading to first-division intervals (t2-tPNf) are associated with greater risk of direct unequal cleavage (DUC) and lower blastulation rate, but not of blastocyst aneuploidy. What is known already During the last decade, time lapse technology (TLT) has made possible detailed and dynamic observation of in vitro embryo development, offering a powerful non-invasive tool for improving embryo selection. Recent evidence suggests that the phase between pronuclear fading and the first cleavage is a perilous bridge between the zygote and the cleaving embryo. Following pronuclear breakdown, delayed progression through mitosis caused by incomplete DNA replication may result in chromosome lagging, whole and segmental segregation errors, and breakage. In this analysis, we specifically tested the hypothesis that delays in a short final phase of fertilization have an impact on embryo development. Study design, size, duration Retrospective study of 1316 zygotes cultured until day 5 using TLT and derived from first ICSI cycles (111 no PGT-A and 94 PGT-A) performed between 2018 and 2022. Testicular sperm or cryopreserved gametes cycles were excluded. DUC was defined as the cleavage of one zygote or blastomere into three daughter blastomeres or cell cycle interval shorter than five hours at the first (DUC1), second (DUC2) or third (DUC3) cell division cycle. Participants/materials, setting, methods t2-tPNf intervals were clustered into quartiles (Q1, &lt;2hpi; Q2, 2–2.5hpi; Q3, 2.5–3hpi and Q4, &gt; 3hpi), which were compared based on embryo development and live birth by linear and logistic regression. The Q1 cluster was set as control, while all other quartiles groups were considered as dummy variables. Multivariate analysis was conducted using the generalized estimating equations, to control for variable numbers of zygotes from each patient. The primary outcome was DUC rate. Main results and the role of chance Overall, rates of DUC1, DUC2 and DUC 3 rates were 10.3%, 8% and 2.9%, respectively. After adjusting for major confounders (maternal age, paternal age, maternal body mass index, cause of infertility, anti-Müllerian hormone, follicle stimulating hormone) we observed a higher DUC 1 and DUC 2 rate with increasing times of t2-tPNf. We observed abnormal distribution of DUC1 and DUC2 rate between quartiles groups. Specifically, 89% of DUC1 was shown among Q3 (21%) and Q4 (78%) and 61% of DUC2 among Q3 (22%) and Q4 (39%). The percentage of DUC1 was higher for Q3 (multivariate-OR = 3.29, 95%CI 0.99-10.85, adjusted-p = 0.01) and Q4 (multivariate-OR = 22.79, 95%CI 7.80-66.62, adjusted-p&lt;0.01]. The percentage of DUC2 was higher only in Q4 (multivariate-OR = 2.58, 95%CI 1.13-5-57, adjusted-p = 0.01). Moreover, we observed a reduced blastulation rate in Q3 (multivariate-OR = 0.68, 95%CI 0.49-0.95, adjusted-p = 0.01) and Q4 (multivariate-OR = 0.27, 95%CI 0.19-0.38, adjusted-p&lt;0.01). No significant differences were observed in DUC3 rate, top quality blastocyst, euploidy rate (PGT-A cycles analysis) and live birth rate. Limitations, reasons for caution The retrospective single-centre design and the limited sample size are limitations of the study Wider implications of the findings The study is consistent with the emerging view that the final phases of fertilization - although short - are crucial for development. Delays in progression towards mitosis probably express chromosome integrity loss and perturbances of chromosome segregation. In most embryos, such perturbances appear to cause trichotomous mitoses and cleavage arrest. Trial registration number Not applicable" @default.
• W4381619877 created "2023-06-23" @default.
• W4381619877 creator A5004535314 @default.
• W4381619877 creator A5006461407 @default.
• W4381619877 creator A5013276814 @default.
• W4381619877 creator A5021146244 @default.
• W4381619877 creator A5030044929 @default.
• W4381619877 creator A5051476729 @default.
• W4381619877 creator A5054595133 @default.
• W4381619877 creator A5058873794 @default.
• W4381619877 creator A5066776601 @default.
• W4381619877 creator A5088384058 @default.
• W4381619877 date "2023-06-01" @default.
• W4381619877 modified "2023-09-23" @default.
• W4381619877 title "P-146 Larger time intervals between pronuclear fading and the first mitosis are strongly associated with trichotomous mitoses and cleavage arrest in human embryos" @default.
• W4381619877 doi "https://doi.org/10.1093/humrep/dead093.510" @default.
• W4381619877 hasPublicationYear "2023" @default.
• W4381619877 type Work @default.
• W4381619877 citedByCount "0" @default.
• W4381619877 crossrefType "journal-article" @default.
• W4381619877 hasAuthorship W4381619877A5004535314 @default.
• W4381619877 hasAuthorship W4381619877A5006461407 @default.
• W4381619877 hasAuthorship W4381619877A5013276814 @default.
• W4381619877 hasAuthorship W4381619877A5021146244 @default.
• W4381619877 hasAuthorship W4381619877A5030044929 @default.
• W4381619877 hasAuthorship W4381619877A5051476729 @default.
• W4381619877 hasAuthorship W4381619877A5054595133 @default.
• W4381619877 hasAuthorship W4381619877A5058873794 @default.
• W4381619877 hasAuthorship W4381619877A5066776601 @default.
• W4381619877 hasAuthorship W4381619877A5088384058 @default.
• W4381619877 hasBestOaLocation W43816198771 @default.
• W4381619877 hasConcept C104317684 @default.
• W4381619877 hasConcept C16685009 @default.
• W4381619877 hasConcept C196843134 @default.
• W4381619877 hasConcept C2778177303 @default.
• W4381619877 hasConcept C2779672484 @default.
• W4381619877 hasConcept C28748434 @default.
• W4381619877 hasConcept C30481170 @default.
• W4381619877 hasConcept C54355233 @default.
• W4381619877 hasConcept C68760520 @default.
• W4381619877 hasConcept C71924100 @default.
• W4381619877 hasConcept C86803240 @default.
• W4381619877 hasConcept C87073359 @default.
• W4381619877 hasConcept C88972607 @default.
• W4381619877 hasConceptScore W4381619877C104317684 @default.
• W4381619877 hasConceptScore W4381619877C16685009 @default.
• W4381619877 hasConceptScore W4381619877C196843134 @default.
• W4381619877 hasConceptScore W4381619877C2778177303 @default.
• W4381619877 hasConceptScore W4381619877C2779672484 @default.
• W4381619877 hasConceptScore W4381619877C28748434 @default.
• W4381619877 hasConceptScore W4381619877C30481170 @default.
• W4381619877 hasConceptScore W4381619877C54355233 @default.
• W4381619877 hasConceptScore W4381619877C68760520 @default.
• W4381619877 hasConceptScore W4381619877C71924100 @default.
• W4381619877 hasConceptScore W4381619877C86803240 @default.
• W4381619877 hasConceptScore W4381619877C87073359 @default.
• W4381619877 hasConceptScore W4381619877C88972607 @default.
• W4381619877 hasIssue "Supplement_1" @default.
• W4381619877 hasLocation W43816198771 @default.
• W4381619877 hasOpenAccess W4381619877 @default.
• W4381619877 hasPrimaryLocation W43816198771 @default.
• W4381619877 hasRelatedWork W1997618777 @default.
• W4381619877 hasRelatedWork W2008720052 @default.
• W4381619877 hasRelatedWork W2058310955 @default.
• W4381619877 hasRelatedWork W2059431578 @default.
• W4381619877 hasRelatedWork W2254907923 @default.
• W4381619877 hasRelatedWork W2401808357 @default.
• W4381619877 hasRelatedWork W2509202055 @default.
• W4381619877 hasRelatedWork W2784731000 @default.
• W4381619877 hasRelatedWork W2999846124 @default.
• W4381619877 hasRelatedWork W4220678844 @default.
• W4381619877 hasVolume "38" @default.
• W4381619877 isParatext "false" @default.
• W4381619877 isRetracted "false" @default.
• W4381619877 workType "article" @default.