FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381620145> ?p ?o ?g. }

• W4381620145 abstract "Abstract Study question How does embryo vitrification regulates the transcriptome of preimplantation embryos through epigenetic regulation and how are the effects transmitted to the female offsprings? Summary answer Embryo vitrification changed the DNA methylation level of preimplantation embryo and disturbed glycolipid metabolism of female offsprings in mice. What is known already Vitrification is a type of embryo cryopreservation increasingly frequently used clinically. The effect of embryo freezing on the health of offspring has been widely concerned. Some clinical studies have shown that offspring obtained from frozen embryo transplantation has higher birth weight and may be at higher risk of certain birth defects, increased risk of type 1 diabetes and cancer. But not all studies agree. The offspring safety of vitrification needs further follow-up research. Mouse model is a helpful tool for us to carry up follow-up study and explore mechanism. Study design, size, duration After superovulation and mating of ICR mice, we obtained 8-Cell embryos. In Vitrify Group, the 8-Cell embryos underwent vitrification and thawing and culture in vitro till blastocysts. In InVitro Group, the 8-Cell embryos were directly cultured in vitro till blastocysts. We transplanted these blastocysts into surrogate mice to get offspring of InVitro and Vitrify. Along with offspring of natural conception (NC), we observed their growth and development and glycolipid metabolism until 18 weeks old. Participants/materials, setting, methods Single embryo RNA-seq and Whole Genome Bisulfite Sequencing (WGBS) were performed to get transcriptome and DNA methylation atlas. Immunofluorescence of blastocysts was performed to test level of 5mC/5hmC and Tet2. Glucose and insulin tolerance test (GTT/ITT), serum lipid level test were performed on offspring to examine the glycolipid metabolism status. Liver transmission electron microscope photographs were take to observe ultramicrostructure. Liver RNA-seq and WGBS were performed to verify the variation on pathways concerning glycolipid metabolism. Main results and the role of chance Pseudo-time analysis from RNA-seq of 16-Cell, morula and blastocyst showed a relative developmental time lag in Vitrify. Differentially expressed genes (DEGs) of vitrify group at the blastocyst stage are mainly enriched in lipid metabolism, insulin signal, TOR signal regulation, gamete production related DNA methylation pathways. GSEA analysis revealed down-regulation of insulin response pathway genes. WGBS showed a trend of genome-wide methylation increase in vitrify group. Immunofluorescence confirmed this observation as the fluorescence intensity ratio of 5mC/5hmC was increased. RNA-seq, qPCR and western-blot showed a lower expression level of Tet2. These results suggested that the demethylation was insufficient in vitrify group. Blastocyst differentially methylated genes (DMRs) from WGBS were enriched in insulin response pathways. In offspring, birth weight of Vitrify offspring is higher than InVitro. GTT and ITT showed an elevated level of AUC in Vitrify 10-week-female, indicating an impaired glucose tolerance. And the fasting serum cholesterol level was elevated in Vitrify 10-week-female, indicating an impaired lipid metabolism. Under transmission electron microscope, we saw swollen mitochondria in liver of Vitrify 10-week-female. We extracted RNA from 10-week-female for RNA-seq, DEGs were enriched in cholesterol biosynthesis pathway. We extracted DNA from 10-week-female for WGBS, DEGs were enriched in cAMP and AMPK signaling pathway. Limitations, reasons for caution We have not confirm the precise molecular site affected by vitrification, clarify the mechanism and conduct the rescue test yet, which we are currently working on. This study is just the first step in this direction, and more efforts are needed. Wider implications of the findings Although more clinical trials and basic science studies are required, our findings provide valuable information for preventive and clinical decisions, as ‘freeze-all’ strategy springs up in many centers. Embryo vitrification needs more cautious consideration. Optimizing embryo vitrification protocols are needed according to the mechanism to minimize unwanted effects. Trial registration number 2021YFC2700601" @default.
• W4381620145 created "2023-06-23" @default.
• W4381620145 creator A5083868441 @default.
• W4381620145 creator A5086260578 @default.
• W4381620145 date "2023-06-01" @default.
• W4381620145 modified "2023-09-23" @default.
• W4381620145 title "P-788 Embryo vitrification affects the epigenome of preimplantation embryos and the effects are inherited by the female offsprings." @default.
• W4381620145 doi "https://doi.org/10.1093/humrep/dead093.1097" @default.
• W4381620145 hasPublicationYear "2023" @default.
• W4381620145 type Work @default.
• W4381620145 citedByCount "0" @default.
• W4381620145 crossrefType "journal-article" @default.
• W4381620145 hasAuthorship W4381620145A5083868441 @default.
• W4381620145 hasAuthorship W4381620145A5086260578 @default.
• W4381620145 hasBestOaLocation W43816201451 @default.
• W4381620145 hasConcept C104317684 @default.
• W4381620145 hasConcept C112672928 @default.
• W4381620145 hasConcept C117717151 @default.
• W4381620145 hasConcept C150194340 @default.
• W4381620145 hasConcept C159399396 @default.
• W4381620145 hasConcept C160099875 @default.
• W4381620145 hasConcept C16685009 @default.
• W4381620145 hasConcept C179933525 @default.
• W4381620145 hasConcept C190727270 @default.
• W4381620145 hasConcept C196843134 @default.
• W4381620145 hasConcept C2778022349 @default.
• W4381620145 hasConcept C2778177303 @default.
• W4381620145 hasConcept C2779234561 @default.
• W4381620145 hasConcept C2779335785 @default.
• W4381620145 hasConcept C41091548 @default.
• W4381620145 hasConcept C54355233 @default.
• W4381620145 hasConcept C71924100 @default.
• W4381620145 hasConcept C86803240 @default.
• W4381620145 hasConcept C87073359 @default.
• W4381620145 hasConceptScore W4381620145C104317684 @default.
• W4381620145 hasConceptScore W4381620145C112672928 @default.
• W4381620145 hasConceptScore W4381620145C117717151 @default.
• W4381620145 hasConceptScore W4381620145C150194340 @default.
• W4381620145 hasConceptScore W4381620145C159399396 @default.
• W4381620145 hasConceptScore W4381620145C160099875 @default.
• W4381620145 hasConceptScore W4381620145C16685009 @default.
• W4381620145 hasConceptScore W4381620145C179933525 @default.
• W4381620145 hasConceptScore W4381620145C190727270 @default.
• W4381620145 hasConceptScore W4381620145C196843134 @default.
• W4381620145 hasConceptScore W4381620145C2778022349 @default.
• W4381620145 hasConceptScore W4381620145C2778177303 @default.
• W4381620145 hasConceptScore W4381620145C2779234561 @default.
• W4381620145 hasConceptScore W4381620145C2779335785 @default.
• W4381620145 hasConceptScore W4381620145C41091548 @default.
• W4381620145 hasConceptScore W4381620145C54355233 @default.
• W4381620145 hasConceptScore W4381620145C71924100 @default.
• W4381620145 hasConceptScore W4381620145C86803240 @default.
• W4381620145 hasConceptScore W4381620145C87073359 @default.
• W4381620145 hasIssue "Supplement_1" @default.
• W4381620145 hasLocation W43816201451 @default.
• W4381620145 hasOpenAccess W4381620145 @default.
• W4381620145 hasPrimaryLocation W43816201451 @default.
• W4381620145 hasRelatedWork W1973193662 @default.
• W4381620145 hasRelatedWork W2000619780 @default.
• W4381620145 hasRelatedWork W2007651035 @default.
• W4381620145 hasRelatedWork W2067986828 @default.
• W4381620145 hasRelatedWork W2124585179 @default.
• W4381620145 hasRelatedWork W2127928418 @default.
• W4381620145 hasRelatedWork W2132619831 @default.
• W4381620145 hasRelatedWork W2218900768 @default.
• W4381620145 hasRelatedWork W2744507534 @default.
• W4381620145 hasRelatedWork W3142523973 @default.
• W4381620145 hasVolume "38" @default.
• W4381620145 isParatext "false" @default.
• W4381620145 isRetracted "false" @default.
• W4381620145 workType "article" @default.