FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381620171> ?p ?o ?g. }

• W4381620171 abstract "Abstract Study question To determine whether hypoxic ovarian damage is due to increased growth and follicles “burnout” or due increased apoptosis, and whether this damage is age dependent. Summary answer Direct tissue hypoxia causes activation and recruitment of dormant follicles without apoptotic changes. This effect is age dependent, more pronounced in adult- versus newborn ovaries. What is known already In a previous study we have shown that perinatal hypoxia causes premature activation and growth initiation of dormant follicles leading to diminished ovarian reserve. Other reports have also linked intrauterine deprivation conditions, premature delivery and small for gestational age newborns with diminished ovarian reserve due to premature ovarian follicular recruitment and exhaustion. Nevertheless, an indirect mechanism influencing ovarian follicle recruitment under hypoxic conditions, such as the release of stress related hormones cannot be ruled out. Study design, size, duration Animal studies were carried out using adult 6-week-old (n = 8) and one-day-old newborn (n = 20) ICR (CD-1) female mice. Throughout the study, animals were housed with an alternating 12-h light/dark cycle with access to food and water ad libitum, under constant room humidity and temperature. Animals were sacrificed and ovaries harvested and immediately cultured in Leibovitz media with L-Glutamine and 10% Fetal Bovine Serum. Participants/materials, setting, methods Ovarian tissue from dams and pups was cultured for 3 hours at 37oC to hypoxia (1% O2 and 99% N2) or normoxia (21% O2 and 5% CO2). Afterwards tissue was embedded in 4% formaldehyde for further processing and analyses. Sections were stained with H&E for follicular counts. For immunohistochemistry, sections were stained with Ki-67 (proliferation marker), anti-Caspase 3 and anti-FOXOp (apoptosis markers). Main results and the role of chance Hypoxia led to a significant decrease in the percentage of primordial follicles out of total follicles as compared to normoxia, both among dams and among pups (3.17±2.75% vs. 17.89±4.4%; p = 0.004 and 40.59±14.88 vs. 81.92±31.56%, p = 0.001 respectively). This was accompanied by a concomitant increases in the percentages of growing (primary and secondary) follicles. Strikingly, this effect was more pronounced in adult dams as compared to that in newborn pups (6 fold versus 2 fold respectively). Ki67 staining indicated increased cell proliferation scores in follicular granulosa cells following hypoxia as compared to normoxia . Both Caspase 3 and FoxoP staining did not detect any changes in both markers of apoptosis either in oocytes, granulosa cells, theca cells or in stromal cells when ovaries were exposed to hypoxia as compared to normoxia. Limitations, reasons for caution This model was performed in mice and generalization to human ovarian physiology remains to be determined. Wider implications of the findings We hereby show that direct tissue hypoxia causes premature activation and growth initiation of dormant follicles without concomitant changes in apoptosis. This effect is age dependent being more pronounced in adult ovaries. Taken together it supports follicular “burn out” as a potential mechanism for hypoxia induced loss of ovarian reserve. Trial registration number IL-117-08-2017" @default.
• W4381620171 created "2023-06-23" @default.
• W4381620171 creator A5012300641 @default.
• W4381620171 creator A5023178314 @default.
• W4381620171 creator A5035586170 @default.
• W4381620171 creator A5046086924 @default.
• W4381620171 creator A5055463181 @default.
• W4381620171 creator A5079837669 @default.
• W4381620171 date "2023-06-01" @default.
• W4381620171 modified "2023-09-23" @default.
• W4381620171 title "P-605 Hypoxia leads to diminished ovarian reserve in an age dependent manner" @default.
• W4381620171 doi "https://doi.org/10.1093/humrep/dead093.934" @default.
• W4381620171 hasPublicationYear "2023" @default.
• W4381620171 type Work @default.
• W4381620171 citedByCount "0" @default.
• W4381620171 crossrefType "journal-article" @default.
• W4381620171 hasAuthorship W4381620171A5012300641 @default.
• W4381620171 hasAuthorship W4381620171A5023178314 @default.
• W4381620171 hasAuthorship W4381620171A5035586170 @default.
• W4381620171 hasAuthorship W4381620171A5046086924 @default.
• W4381620171 hasAuthorship W4381620171A5055463181 @default.
• W4381620171 hasAuthorship W4381620171A5079837669 @default.
• W4381620171 hasBestOaLocation W43816201711 @default.
• W4381620171 hasConcept C126322002 @default.
• W4381620171 hasConcept C134018914 @default.
• W4381620171 hasConcept C16685009 @default.
• W4381620171 hasConcept C178790620 @default.
• W4381620171 hasConcept C185592680 @default.
• W4381620171 hasConcept C187785154 @default.
• W4381620171 hasConcept C1998276 @default.
• W4381620171 hasConcept C2776600139 @default.
• W4381620171 hasConcept C2777688143 @default.
• W4381620171 hasConcept C2778376644 @default.
• W4381620171 hasConcept C2779234561 @default.
• W4381620171 hasConcept C2780536345 @default.
• W4381620171 hasConcept C42407357 @default.
• W4381620171 hasConcept C540031477 @default.
• W4381620171 hasConcept C54355233 @default.
• W4381620171 hasConcept C71924100 @default.
• W4381620171 hasConcept C7836513 @default.
• W4381620171 hasConcept C86803240 @default.
• W4381620171 hasConceptScore W4381620171C126322002 @default.
• W4381620171 hasConceptScore W4381620171C134018914 @default.
• W4381620171 hasConceptScore W4381620171C16685009 @default.
• W4381620171 hasConceptScore W4381620171C178790620 @default.
• W4381620171 hasConceptScore W4381620171C185592680 @default.
• W4381620171 hasConceptScore W4381620171C187785154 @default.
• W4381620171 hasConceptScore W4381620171C1998276 @default.
• W4381620171 hasConceptScore W4381620171C2776600139 @default.
• W4381620171 hasConceptScore W4381620171C2777688143 @default.
• W4381620171 hasConceptScore W4381620171C2778376644 @default.
• W4381620171 hasConceptScore W4381620171C2779234561 @default.
• W4381620171 hasConceptScore W4381620171C2780536345 @default.
• W4381620171 hasConceptScore W4381620171C42407357 @default.
• W4381620171 hasConceptScore W4381620171C540031477 @default.
• W4381620171 hasConceptScore W4381620171C54355233 @default.
• W4381620171 hasConceptScore W4381620171C71924100 @default.
• W4381620171 hasConceptScore W4381620171C7836513 @default.
• W4381620171 hasConceptScore W4381620171C86803240 @default.
• W4381620171 hasIssue "Supplement_1" @default.
• W4381620171 hasLocation W43816201711 @default.
• W4381620171 hasOpenAccess W4381620171 @default.
• W4381620171 hasPrimaryLocation W43816201711 @default.
• W4381620171 hasRelatedWork W1966283443 @default.
• W4381620171 hasRelatedWork W2000124881 @default.
• W4381620171 hasRelatedWork W2013275608 @default.
• W4381620171 hasRelatedWork W2014570372 @default.
• W4381620171 hasRelatedWork W2036659686 @default.
• W4381620171 hasRelatedWork W2057217014 @default.
• W4381620171 hasRelatedWork W2101390122 @default.
• W4381620171 hasRelatedWork W2149573140 @default.
• W4381620171 hasRelatedWork W2151539963 @default.
• W4381620171 hasRelatedWork W2172100709 @default.
• W4381620171 hasVolume "38" @default.
• W4381620171 isParatext "false" @default.
• W4381620171 isRetracted "false" @default.
• W4381620171 workType "article" @default.