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- W4381805655 abstract "Objective: This study aimed to identify the single-nucleotide polymorphisms (SNPs) in the dual-specificity phosphatase 8 (<i>DUSP8</i>) and insulin-like growth factor 2 (<i>IGF2</i>) genes and to explore their effects on inosine-5′-monophosphate (IMP), inosine, and hypoxanthine contents in Korean native chicken -red-brown line (KNC-R Line).Methods: A total sample of 284 (males, n = 127; females n = 157) and 230 (males, n = 106; females, n = 124) aged of 10 weeks old KNC-R line was used for genotyping of <i>DUSP8</i> and <i>IGF2</i> genes, respectively. One SNP (rs313443014 C>T) in <i>DUSP8</i> gene and two SNPs (rs315806609A/G and rs313810945T/C) in IGF2 gene were used for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and KASP methods, respectively. The Two-way analysis of variance of the R program was used to associate DUSP8 and IGF2 genotypes with nucleotide contents in KNC-R chickens.Results: The DUSP8 (rs313443014 C>T) was polymorphic in KNC-R line and showed three genotypes: CC, CT, and TT. The <i>IGF2</i> gene (rs315806609A/G and rs313810945T/C) was also polymorphic and had three genotypes per SNP, including GG, AG, and AA for the SNP rs315806609A/G and genotypes: CC, CT, and TT for the SNP rs313810945T/C. Association resulted into a strong significant association (p<0.01) with IMP, inosine, and hypoxanthine. Moreover, the significant effect of sex (p<0.05) on nucleotide content was also observed.Conclusion: The SNPs in the <i>DUSP8</i> and <i>IGF2</i> genes might be used as genetic markers in the selection and production of chickens with highly flavored meat." @default.
- W4381805655 created "2023-06-24" @default.
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- W4381805655 date "2023-09-01" @default.
- W4381805655 modified "2023-10-02" @default.
- W4381805655 title "Association of single-nucleotide polymorphisms in dual specificity phosphatase 8 (DUSP8) and insulin-like growth factor 2 (IGF2) genes with inosine-5′-monophosphate, inosine, and hypoxanthine contents in chickens" @default.
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- W4381805655 doi "https://doi.org/10.5713/ab.23.0080" @default.
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