Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381857089> ?p ?o ?g. }
- W4381857089 abstract "Transient receptor potential ion channel, vanilloid subfamily, type 1 (TRPV1) cation channel, and cannabinoid receptor 1 (CB 1 ) are essential in the modulation of nociceptive signaling in the spinal cord dorsal horn that underlies different pathological pain states. TRPV1 and CB 1 receptors share the endogenous agonist anandamide (AEA), produced from N-arachidonoylphosphatidylethanolamine (20:4-NAPE). We investigated the effect of the anandamide precursor 20:4-NAPE on synaptic activity in naive and inflammatory conditions. Patch-clamp recordings of miniature excitatory postsynaptic currents (mEPSCs) from superficial dorsal horn neurons in rat acute spinal cord slices were used. Peripheral inflammation was induced by subcutaneous injection of carrageenan. Under naive conditions, mEPSCs frequency (0.96 ± 0.11 Hz) was significantly decreased after 20 μM 20:4-NAPE application (55.3 ± 7.4%). This 20:4-NAPE-induced inhibition was blocked by anandamide-synthesizing enzyme N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) inhibitor LEI-401. In addition, the inhibition was prevented by the CB 1 receptor antagonist PF 514273 (0.2 μM) but not by the TRPV1 receptor antagonist SB 366791 (10 μM). Under inflammatory conditions, 20:4-NAPE (20 μM) also exhibited a significant inhibitory effect (74.5 ± 8.9%) on the mEPSCs frequency that was prevented by the TRPV1 receptor antagonist SB 366791 but not by PF 514273 application. Our results show that 20:4-NAPE application has a significant modulatory effect on spinal cord nociceptive signaling that is mediated by both TRPV1 and CB 1 presynaptic receptors, whereas peripheral inflammation changes the underlying mechanism. The switch between TRPV1 and CB 1 receptor activation by the AEA precursor 20:4-NAPE during inflammation may play an important role in nociceptive processing, hence the development of pathological pain." @default.
- W4381857089 created "2023-06-25" @default.
- W4381857089 creator A5002228313 @default.
- W4381857089 creator A5014541625 @default.
- W4381857089 creator A5019348997 @default.
- W4381857089 creator A5021135908 @default.
- W4381857089 creator A5086119890 @default.
- W4381857089 creator A5088095716 @default.
- W4381857089 creator A5092252755 @default.
- W4381857089 date "2023-06-22" @default.
- W4381857089 modified "2023-10-16" @default.
- W4381857089 title "Inhibition of synaptic transmission by anandamide precursor 20:4-NAPE is mediated by TRPV1 receptors under inflammatory conditions" @default.
- W4381857089 cites W137978099 @default.
- W4381857089 cites W1488329806 @default.
- W4381857089 cites W1495617953 @default.
- W4381857089 cites W1520028124 @default.
- W4381857089 cites W1549092682 @default.
- W4381857089 cites W1660934519 @default.
- W4381857089 cites W1963531856 @default.
- W4381857089 cites W1965059229 @default.
- W4381857089 cites W1965148914 @default.
- W4381857089 cites W1966960464 @default.
- W4381857089 cites W1967922769 @default.
- W4381857089 cites W1989155012 @default.
- W4381857089 cites W1991999780 @default.
- W4381857089 cites W2001679027 @default.
- W4381857089 cites W2002797425 @default.
- W4381857089 cites W2003987111 @default.
- W4381857089 cites W2009351917 @default.
- W4381857089 cites W2012477863 @default.
- W4381857089 cites W2015829020 @default.
- W4381857089 cites W2020513122 @default.
- W4381857089 cites W2022683321 @default.
- W4381857089 cites W2022737700 @default.
- W4381857089 cites W2023375612 @default.
- W4381857089 cites W2024083629 @default.
- W4381857089 cites W2029263283 @default.
- W4381857089 cites W2033743296 @default.
- W4381857089 cites W2041244325 @default.
- W4381857089 cites W2046586945 @default.
- W4381857089 cites W2048041839 @default.
- W4381857089 cites W2053193214 @default.
- W4381857089 cites W2056943012 @default.
- W4381857089 cites W2059535086 @default.
- W4381857089 cites W2062350830 @default.
- W4381857089 cites W2064581339 @default.
- W4381857089 cites W2071222789 @default.
- W4381857089 cites W2072008056 @default.
- W4381857089 cites W2077336577 @default.
- W4381857089 cites W2081006847 @default.
- W4381857089 cites W2083481324 @default.
- W4381857089 cites W2092206207 @default.
- W4381857089 cites W2099479841 @default.
- W4381857089 cites W2106493645 @default.
- W4381857089 cites W2107705149 @default.
- W4381857089 cites W2109248876 @default.
- W4381857089 cites W2112924959 @default.
- W4381857089 cites W2113960469 @default.
- W4381857089 cites W2137734852 @default.
- W4381857089 cites W2142741697 @default.
- W4381857089 cites W2145865815 @default.
- W4381857089 cites W2170110471 @default.
- W4381857089 cites W2418235693 @default.
- W4381857089 cites W2522358191 @default.
- W4381857089 cites W2563728479 @default.
- W4381857089 cites W2803185382 @default.
- W4381857089 cites W2884605959 @default.
- W4381857089 cites W2900960327 @default.
- W4381857089 cites W3035783703 @default.
- W4381857089 cites W3121930701 @default.
- W4381857089 cites W3143318784 @default.
- W4381857089 cites W3215106127 @default.
- W4381857089 doi "https://doi.org/10.3389/fnmol.2023.1188503" @default.
- W4381857089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37426071" @default.
- W4381857089 hasPublicationYear "2023" @default.
- W4381857089 type Work @default.
- W4381857089 citedByCount "0" @default.
- W4381857089 crossrefType "journal-article" @default.
- W4381857089 hasAuthorship W4381857089A5002228313 @default.
- W4381857089 hasAuthorship W4381857089A5014541625 @default.
- W4381857089 hasAuthorship W4381857089A5019348997 @default.
- W4381857089 hasAuthorship W4381857089A5021135908 @default.
- W4381857089 hasAuthorship W4381857089A5086119890 @default.
- W4381857089 hasAuthorship W4381857089A5088095716 @default.
- W4381857089 hasAuthorship W4381857089A5092252755 @default.
- W4381857089 hasBestOaLocation W43818570891 @default.
- W4381857089 hasConcept C112592302 @default.
- W4381857089 hasConcept C126322002 @default.
- W4381857089 hasConcept C134018914 @default.
- W4381857089 hasConcept C148001335 @default.
- W4381857089 hasConcept C15490471 @default.
- W4381857089 hasConcept C155164980 @default.
- W4381857089 hasConcept C160145004 @default.
- W4381857089 hasConcept C170493617 @default.
- W4381857089 hasConcept C185592680 @default.
- W4381857089 hasConcept C200170125 @default.
- W4381857089 hasConcept C2776885963 @default.
- W4381857089 hasConcept C2778122271 @default.
- W4381857089 hasConcept C2778938600 @default.
- W4381857089 hasConcept C2779245659 @default.