FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381857302> ?p ?o ?g. }

• W4381857302 abstract "Abstract Background Hepatitis B virus (HBV) infection is a global health epidemic that causes fatal complications leading to liver cirrhosis and hepatocellular carcinoma. The link between HBV-related dysbiosis and specific bacterial taxa is still under investigation. Enterocloster is emerging as a new genus (formerly Clostridium ), including Enterocloster bolteae , a gut pathogen previously associated with dysbiosis and human diseases such as autism, multiple sclerosis, and inflammatory bowel diseases. Its role in liver diseases, especially HBV infection, is not reported. Methods We analyzed the fecal samples of eight patients with HBV and ten healthy individuals using the high-throughput culturomics approach compared to 16S rRNA sequencing. Quantification of ethanol, known for its damaging effect on the liver, produced from bacterial strains enriched in HBV was carried out by gas chromatography-mass spectrometry. Results By culturomics, 29,120 isolated colonies were analyzed by MALDI-TOF to identify 340 species (240 species in HBV samples, 254 species in control samples) belonging to 169 genera and six phyla. In the HBV group, 48 species were already known in humans but had not been previously found in the gut, 17 known species not previously found in humans, and six new species were isolated. Comparing bacterial species frequency, we serendipitously found three bacterial genera with significantly enriched bacterial diversity in HBV gut dysbiosis: Enterocloster, Clostridium , and Streptococcus ( p = 0.0016, p = 0.041, p = 0.053, respectively). However, metagenomics could not identify this enrichment, possibly concerning its insufficient taxonomical resolution (equivocal assignment of operational taxonomic units). At the species level, significantly enriched species in HBV almost all belonged to class Clostridia as Clostridium perfringens, Clostridium sporogenes, Enterocloster aldenensis, Enterocloster bolteae, Enterocloster clostridioformis , and Clostridium innocuum. Two E. bolteae strains, isolated from two different HBV patients, showed high ethanol production (27 and 200 mM). Conclusion Culturomics allowed us to identify Enterocloster species, and specifically E. bolteae , that are enriched in the gut microbiota of HBV patients. These species have never been isolated in HBV patients so far. Moreover, ethanol production by E. Boltea strains isolated from HBV patients could play a role in liver disease progression. Additionally, culturomics might be critical for better elucidating the relationship between dysbiosis and HBV infection in the future." @default.
• W4381857302 created "2023-06-25" @default.
• W4381857302 creator A5001607188 @default.
• W4381857302 creator A5005841492 @default.
• W4381857302 creator A5029772514 @default.
• W4381857302 creator A5033487398 @default.
• W4381857302 creator A5038686513 @default.
• W4381857302 creator A5045734142 @default.
• W4381857302 creator A5065165683 @default.
• W4381857302 creator A5070423831 @default.
• W4381857302 creator A5074730004 @default.
• W4381857302 creator A5082013876 @default.
• W4381857302 creator A5085022755 @default.
• W4381857302 date "2023-06-22" @default.
• W4381857302 modified "2023-10-18" @default.
• W4381857302 title "Ethanol-Producing Enterocloster bolteae is enriched in Hepatitis B Virus-Associated Gut Dysbiosis: A Case-Control Culturomics Study" @default.
• W4381857302 cites W1760389142 @default.
• W4381857302 cites W1896653510 @default.
• W4381857302 cites W1980100361 @default.
• W4381857302 cites W1992804695 @default.
• W4381857302 cites W1999919570 @default.
• W4381857302 cites W2005644104 @default.
• W4381857302 cites W2012755914 @default.
• W4381857302 cites W2016931366 @default.
• W4381857302 cites W2023615726 @default.
• W4381857302 cites W2035101310 @default.
• W4381857302 cites W2035378093 @default.
• W4381857302 cites W2039675240 @default.
• W4381857302 cites W2051567523 @default.
• W4381857302 cites W2065465329 @default.
• W4381857302 cites W2090336467 @default.
• W4381857302 cites W2098313813 @default.
• W4381857302 cites W2107414352 @default.
• W4381857302 cites W2109886572 @default.
• W4381857302 cites W2121055398 @default.
• W4381857302 cites W2123008311 @default.
• W4381857302 cites W2136835255 @default.
• W4381857302 cites W2142095828 @default.
• W4381857302 cites W2148277575 @default.
• W4381857302 cites W2155897723 @default.
• W4381857302 cites W2156255024 @default.
• W4381857302 cites W2169793467 @default.
• W4381857302 cites W2172112723 @default.
• W4381857302 cites W2400777527 @default.
• W4381857302 cites W2522316027 @default.
• W4381857302 cites W2526966274 @default.
• W4381857302 cites W2535360044 @default.
• W4381857302 cites W2549670165 @default.
• W4381857302 cites W2561736761 @default.
• W4381857302 cites W2563720483 @default.
• W4381857302 cites W2567594783 @default.
• W4381857302 cites W2608515760 @default.
• W4381857302 cites W2754799198 @default.
• W4381857302 cites W2801167100 @default.
• W4381857302 cites W2802886512 @default.
• W4381857302 cites W2807431832 @default.
• W4381857302 cites W2809322052 @default.
• W4381857302 cites W2889240798 @default.
• W4381857302 cites W2930497553 @default.
• W4381857302 cites W2957820784 @default.
• W4381857302 cites W2979620955 @default.
• W4381857302 cites W2990850114 @default.
• W4381857302 cites W3000639794 @default.
• W4381857302 cites W3010832831 @default.
• W4381857302 cites W3015963259 @default.
• W4381857302 cites W3016203278 @default.
• W4381857302 cites W3031602500 @default.
• W4381857302 cites W3097003771 @default.
• W4381857302 cites W3097318844 @default.
• W4381857302 cites W3107716232 @default.
• W4381857302 cites W3111795541 @default.
• W4381857302 cites W3112654886 @default.
• W4381857302 cites W3116950956 @default.
• W4381857302 cites W3134956600 @default.
• W4381857302 cites W3135299124 @default.
• W4381857302 cites W3137916113 @default.
• W4381857302 cites W3156129427 @default.
• W4381857302 cites W3168099987 @default.
• W4381857302 cites W3184078141 @default.
• W4381857302 cites W3196733728 @default.
• W4381857302 cites W3198797501 @default.
• W4381857302 cites W3207184595 @default.
• W4381857302 cites W3208287334 @default.
• W4381857302 cites W4211026375 @default.
• W4381857302 cites W4292528167 @default.
• W4381857302 cites W4295196917 @default.
• W4381857302 cites W4296098835 @default.
• W4381857302 cites W4307633838 @default.
• W4381857302 cites W4311612274 @default.
• W4381857302 cites W4361297857 @default.
• W4381857302 cites W4362454179 @default.
• W4381857302 doi "https://doi.org/10.21203/rs.3.rs-3066403/v1" @default.
• W4381857302 hasPublicationYear "2023" @default.
• W4381857302 type Work @default.
• W4381857302 citedByCount "0" @default.
• W4381857302 crossrefType "posted-content" @default.
• W4381857302 hasAuthorship W4381857302A5001607188 @default.
• W4381857302 hasAuthorship W4381857302A5005841492 @default.
• W4381857302 hasAuthorship W4381857302A5029772514 @default.
• W4381857302 hasAuthorship W4381857302A5033487398 @default.

• next