FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381889992> ?p ?o ?g. }

• W4381889992 abstract "Hyaluronic acid (HA), composed of glucuronic acid (GlcUA) and N-acetyl glucoseamine (GlcNAc), is a versatile biopolymer with high commercial value and innumerous physiological roles and pharmaceutical applications. The hasA gene has main role in HA biosynthesis by Streptococcus strain as a natural producer. The hasB and hasC genes are also mediate GlcUA precursor biosynthesis. In the present study, S. equisimilis hasA gene; B. subtilis tuaD and gtaB genes for GlcUA precursors enhancement, and vgb gene coding bacterial hemoglobin as an oxygen provider were used to construct the B. subtilis strain for HA production. RBSHA (hasA), RBSHA2 (hasA/tuaD/gtaB), and RBSHA3 (hasA/tuaD/gtaB/vgb) strains were developed and confirmed through genotype and phenotype analysis. After HA production and purification, FTIR spectroscopy confirmed the produced HA structures. HA assay showed the highest HA titer for RBSHA3 (2.1 ± 0.18 mg/ml) and then RBSHA2 (1.9 ± 0.03 mg/ml), and RBSHA (0.6 ± 0.14 mg/ml). Statistical analysis indicated there is no significant difference in HA titer between RBSHA2 and RBSHA3 strains (p-value > 0.05), however, these strains produced HA approximately 4-fold higher than that of RBSHA strain. Agarose gel electrophoresis showed the same molecular weight (< 30 kDa) of produced HA by strains. Dynamic light scattering (DLS) revealed all HA polymers had a relatively low polydispersity index (PDI < 0.5). These findings demonstrate the successful GlcUA biosynthetic pathway engineering strategy in improving HA yield by recombinant B. subtilis, metabolically-robust, and industrially potential strain." @default.
• W4381889992 created "2023-06-25" @default.
• W4381889992 creator A5003969274 @default.
• W4381889992 creator A5010549796 @default.
• W4381889992 creator A5041243420 @default.
• W4381889992 creator A5054904599 @default.
• W4381889992 creator A5090218504 @default.
• W4381889992 date "2023-06-24" @default.
• W4381889992 modified "2023-09-25" @default.
• W4381889992 title "The effect of manipulating glucuronic acid biosynthetic pathway in Bacillus subtilis strain on hyaluronic acid production" @default.
• W4381889992 cites W1595780297 @default.
• W4381889992 cites W1678194650 @default.
• W4381889992 cites W1964925190 @default.
• W4381889992 cites W1971262916 @default.
• W4381889992 cites W1983414539 @default.
• W4381889992 cites W1995845287 @default.
• W4381889992 cites W2023864321 @default.
• W4381889992 cites W2048321694 @default.
• W4381889992 cites W2063856596 @default.
• W4381889992 cites W2067171708 @default.
• W4381889992 cites W2086731815 @default.
• W4381889992 cites W2089433978 @default.
• W4381889992 cites W2100491597 @default.
• W4381889992 cites W2102267945 @default.
• W4381889992 cites W2124101452 @default.
• W4381889992 cites W2132046491 @default.
• W4381889992 cites W2158174352 @default.
• W4381889992 cites W2172165245 @default.
• W4381889992 cites W2291204809 @default.
• W4381889992 cites W2416874763 @default.
• W4381889992 cites W2464906938 @default.
• W4381889992 cites W2508237439 @default.
• W4381889992 cites W2760024633 @default.
• W4381889992 cites W2794995305 @default.
• W4381889992 cites W2801885456 @default.
• W4381889992 cites W2895377446 @default.
• W4381889992 cites W2913987910 @default.
• W4381889992 cites W2946754818 @default.
• W4381889992 cites W3008862748 @default.
• W4381889992 cites W3048625306 @default.
• W4381889992 cites W3048921504 @default.
• W4381889992 cites W3149065164 @default.
• W4381889992 cites W3184293886 @default.
• W4381889992 cites W3191236432 @default.
• W4381889992 cites W4200517991 @default.
• W4381889992 cites W4224297613 @default.
• W4381889992 cites W4281749876 @default.
• W4381889992 cites W4285088963 @default.
• W4381889992 doi "https://doi.org/10.1186/s13568-023-01567-2" @default.
• W4381889992 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37354246" @default.
• W4381889992 hasPublicationYear "2023" @default.
• W4381889992 type Work @default.
• W4381889992 citedByCount "0" @default.
• W4381889992 crossrefType "journal-article" @default.
• W4381889992 hasAuthorship W4381889992A5003969274 @default.
• W4381889992 hasAuthorship W4381889992A5010549796 @default.
• W4381889992 hasAuthorship W4381889992A5041243420 @default.
• W4381889992 hasAuthorship W4381889992A5054904599 @default.
• W4381889992 hasAuthorship W4381889992A5090218504 @default.
• W4381889992 hasBestOaLocation W43818899921 @default.
• W4381889992 hasConcept C100544194 @default.
• W4381889992 hasConcept C100817775 @default.
• W4381889992 hasConcept C104317684 @default.
• W4381889992 hasConcept C105702510 @default.
• W4381889992 hasConcept C185592680 @default.
• W4381889992 hasConcept C2776102837 @default.
• W4381889992 hasConcept C2776648616 @default.
• W4381889992 hasConcept C2777272437 @default.
• W4381889992 hasConcept C2777529286 @default.
• W4381889992 hasConcept C2777811704 @default.
• W4381889992 hasConcept C2778022156 @default.
• W4381889992 hasConcept C2781236742 @default.
• W4381889992 hasConcept C523546767 @default.
• W4381889992 hasConcept C54355233 @default.
• W4381889992 hasConcept C553450214 @default.
• W4381889992 hasConcept C55493867 @default.
• W4381889992 hasConcept C86803240 @default.
• W4381889992 hasConceptScore W4381889992C100544194 @default.
• W4381889992 hasConceptScore W4381889992C100817775 @default.
• W4381889992 hasConceptScore W4381889992C104317684 @default.
• W4381889992 hasConceptScore W4381889992C105702510 @default.
• W4381889992 hasConceptScore W4381889992C185592680 @default.
• W4381889992 hasConceptScore W4381889992C2776102837 @default.
• W4381889992 hasConceptScore W4381889992C2776648616 @default.
• W4381889992 hasConceptScore W4381889992C2777272437 @default.
• W4381889992 hasConceptScore W4381889992C2777529286 @default.
• W4381889992 hasConceptScore W4381889992C2777811704 @default.
• W4381889992 hasConceptScore W4381889992C2778022156 @default.
• W4381889992 hasConceptScore W4381889992C2781236742 @default.
• W4381889992 hasConceptScore W4381889992C523546767 @default.
• W4381889992 hasConceptScore W4381889992C54355233 @default.
• W4381889992 hasConceptScore W4381889992C553450214 @default.
• W4381889992 hasConceptScore W4381889992C55493867 @default.
• W4381889992 hasConceptScore W4381889992C86803240 @default.
• W4381889992 hasFunder F4320326567 @default.
• W4381889992 hasIssue "1" @default.
• W4381889992 hasLocation W43818899921 @default.
• W4381889992 hasLocation W43818899922 @default.
• W4381889992 hasLocation W43818899923 @default.
• W4381889992 hasLocation W43818899924 @default.

• next