FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381890147> ?p ?o ?g. }

• W4381890147 abstract "Abstract Cellular metabolism is a key regulator of energetics, cell growth, regeneration and homeostasis. Spatially mapping the heterogeneity of cellular metabolic activity is of great importance for unraveling the overall cell and tissue health. In this regard, imaging the endogenous metabolic co-factors NAD(P)H and FAD with sub-cellular resolution and in a non-invasive manner would be useful to determine tissue and cell viability in a clinical environment, but practical use is limited by current imaging techniques. In this article, we demonstrate the use of phasor-based hyperspectral light-sheet (HS-LS) microscopy using a single UVA excitation wavelength as a route to mapping metabolism in three dimensions. We show that excitation solely at a UVA wavelength of 375 nm can simultaneously excite NAD(P)H and FAD autofluorescence, while their relative contributions can be readily quantified using a hardware-based spectral phasor analysis. We demonstrate the potential of our HS-LS system by capturing dynamic changes in metabolic activity during pre-implantation embryo development. To validate our approach, we delineate metabolic changes during pre-implantation embryo development from volumetric maps of metabolic activity. Importantly, our approach overcomes the need for multiple excitation wavelengths, two-photon imaging or significant post-processing of data, paving the way towards clinical translation, such as in situ, non-invasive assessment of embryo viability." @default.
• W4381890147 created "2023-06-25" @default.
• W4381890147 creator A5018081131 @default.
• W4381890147 creator A5022881056 @default.
• W4381890147 creator A5027381937 @default.
• W4381890147 creator A5032199248 @default.
• W4381890147 creator A5037385828 @default.
• W4381890147 creator A5039901364 @default.
• W4381890147 creator A5044047319 @default.
• W4381890147 creator A5045564453 @default.
• W4381890147 creator A5055769532 @default.
• W4381890147 creator A5085173358 @default.
• W4381890147 date "2023-06-22" @default.
• W4381890147 modified "2023-10-16" @default.
• W4381890147 title "UVA hyperspectral light-sheet microscopy for volumetric metabolic imaging: application to pre-implantation embryo development" @default.
• W4381890147 cites W1861626221 @default.
• W4381890147 cites W1993671248 @default.
• W4381890147 cites W2000839241 @default.
• W4381890147 cites W2002402687 @default.
• W4381890147 cites W2033007699 @default.
• W4381890147 cites W2042769315 @default.
• W4381890147 cites W2050615594 @default.
• W4381890147 cites W2077082129 @default.
• W4381890147 cites W2083147890 @default.
• W4381890147 cites W2096105943 @default.
• W4381890147 cites W2121388227 @default.
• W4381890147 cites W2129827453 @default.
• W4381890147 cites W2133362850 @default.
• W4381890147 cites W2145734069 @default.
• W4381890147 cites W2302236988 @default.
• W4381890147 cites W2491458126 @default.
• W4381890147 cites W2507604610 @default.
• W4381890147 cites W2558664056 @default.
• W4381890147 cites W2572957424 @default.
• W4381890147 cites W2889675349 @default.
• W4381890147 cites W2993754136 @default.
• W4381890147 cites W3015780231 @default.
• W4381890147 cites W3092006458 @default.
• W4381890147 cites W3100377772 @default.
• W4381890147 cites W3133543132 @default.
• W4381890147 cites W3177018805 @default.
• W4381890147 cites W3212864668 @default.
• W4381890147 cites W4206512325 @default.
• W4381890147 cites W4206723552 @default.
• W4381890147 cites W4210343384 @default.
• W4381890147 cites W4293217198 @default.
• W4381890147 cites W4362597447 @default.
• W4381890147 doi "https://doi.org/10.1101/2023.06.21.545939" @default.
• W4381890147 hasPublicationYear "2023" @default.
• W4381890147 type Work @default.
• W4381890147 citedByCount "0" @default.
• W4381890147 crossrefType "posted-content" @default.
• W4381890147 hasAuthorship W4381890147A5018081131 @default.
• W4381890147 hasAuthorship W4381890147A5022881056 @default.
• W4381890147 hasAuthorship W4381890147A5027381937 @default.
• W4381890147 hasAuthorship W4381890147A5032199248 @default.
• W4381890147 hasAuthorship W4381890147A5037385828 @default.
• W4381890147 hasAuthorship W4381890147A5039901364 @default.
• W4381890147 hasAuthorship W4381890147A5044047319 @default.
• W4381890147 hasAuthorship W4381890147A5045564453 @default.
• W4381890147 hasAuthorship W4381890147A5055769532 @default.
• W4381890147 hasAuthorship W4381890147A5085173358 @default.
• W4381890147 hasBestOaLocation W43818901471 @default.
• W4381890147 hasConcept C120665830 @default.
• W4381890147 hasConcept C121332964 @default.
• W4381890147 hasConcept C12554922 @default.
• W4381890147 hasConcept C147080431 @default.
• W4381890147 hasConcept C1491633281 @default.
• W4381890147 hasConcept C154945302 @default.
• W4381890147 hasConcept C159078339 @default.
• W4381890147 hasConcept C163258240 @default.
• W4381890147 hasConcept C176605952 @default.
• W4381890147 hasConcept C178790620 @default.
• W4381890147 hasConcept C179687394 @default.
• W4381890147 hasConcept C181199279 @default.
• W4381890147 hasConcept C185592680 @default.
• W4381890147 hasConcept C186060115 @default.
• W4381890147 hasConcept C196843134 @default.
• W4381890147 hasConcept C2777822432 @default.
• W4381890147 hasConcept C2994212657 @default.
• W4381890147 hasConcept C41008148 @default.
• W4381890147 hasConcept C47785574 @default.
• W4381890147 hasConcept C53227056 @default.
• W4381890147 hasConcept C55493867 @default.
• W4381890147 hasConcept C62231903 @default.
• W4381890147 hasConcept C62520636 @default.
• W4381890147 hasConcept C75520062 @default.
• W4381890147 hasConcept C86803240 @default.
• W4381890147 hasConcept C89227174 @default.
• W4381890147 hasConcept C91881484 @default.
• W4381890147 hasConcept C95444343 @default.
• W4381890147 hasConceptScore W4381890147C120665830 @default.
• W4381890147 hasConceptScore W4381890147C121332964 @default.
• W4381890147 hasConceptScore W4381890147C12554922 @default.
• W4381890147 hasConceptScore W4381890147C147080431 @default.
• W4381890147 hasConceptScore W4381890147C1491633281 @default.
• W4381890147 hasConceptScore W4381890147C154945302 @default.
• W4381890147 hasConceptScore W4381890147C159078339 @default.
• W4381890147 hasConceptScore W4381890147C163258240 @default.
• W4381890147 hasConceptScore W4381890147C176605952 @default.

• next