Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381893017> ?p ?o ?g. }
- W4381893017 endingPage "109677" @default.
- W4381893017 startingPage "109677" @default.
- W4381893017 abstract "The low-dose combination of Arsenite (As3+) and selenite (Se4+) has the advantages of lower biological toxicity and better curative effects for acute promyelocytic leukemia (APL) therapy. However, the underlying mechanisms remain unclear. Here, based on the fact that the combination of 2 μM A3+ plus 4 μM Se4+ possessed a stronger anti-leukemic effect on APL cell line NB4 as compared with each individual, we employed iTRAQ-based quantitative proteomics to identify a total of 58 proteins that were differentially expressed after treatment with As3+/Se4+ combination rather than As3+ or Se4+ alone, the majority of which were involved in spliceosome pathway. Among them, eight proteins stood out by virtue of their splicing function and significant changes. They were validated as being decreased in mRNA and protein levels under As3+/Se4+ combination treatment. Further functional studies showed that only knockdown of two splicing factors, SF3A3 and SRSF5, suppressed the growth of NB4 cells. The reduction of SF3A3 was found to cause G1/S cell cycle arrest, which resulted in proliferation inhibition. Moreover, SRSF5 downregulation induced cell apoptosis through the activation of caspase-3. Taken together, these findings indicate that SF3A3 and SRSF5 function as pro-leukemic factors and can be potential novel therapeutic targets for APL." @default.
- W4381893017 created "2023-06-25" @default.
- W4381893017 creator A5000813056 @default.
- W4381893017 creator A5004761771 @default.
- W4381893017 creator A5011455054 @default.
- W4381893017 creator A5079078165 @default.
- W4381893017 date "2023-07-01" @default.
- W4381893017 modified "2023-10-12" @default.
- W4381893017 title "Inhibition of splicing factors SF3A3 and SRSF5 contributes to As3+/Se4+ combination-mediated proliferation suppression and apoptosis induction in acute promyelocytic leukemia cells" @default.
- W4381893017 cites W1263857580 @default.
- W4381893017 cites W1525380511 @default.
- W4381893017 cites W1536775300 @default.
- W4381893017 cites W1745634660 @default.
- W4381893017 cites W1829675031 @default.
- W4381893017 cites W1948328750 @default.
- W4381893017 cites W1964924007 @default.
- W4381893017 cites W1967415817 @default.
- W4381893017 cites W1970554957 @default.
- W4381893017 cites W1971846931 @default.
- W4381893017 cites W1974250897 @default.
- W4381893017 cites W1975412826 @default.
- W4381893017 cites W1977257967 @default.
- W4381893017 cites W1977301794 @default.
- W4381893017 cites W1980961407 @default.
- W4381893017 cites W1981777405 @default.
- W4381893017 cites W1991406325 @default.
- W4381893017 cites W1993236149 @default.
- W4381893017 cites W2000567082 @default.
- W4381893017 cites W2003250511 @default.
- W4381893017 cites W2004346657 @default.
- W4381893017 cites W2005844899 @default.
- W4381893017 cites W2006025476 @default.
- W4381893017 cites W2010216204 @default.
- W4381893017 cites W2010852376 @default.
- W4381893017 cites W2011040786 @default.
- W4381893017 cites W2014210334 @default.
- W4381893017 cites W2023546573 @default.
- W4381893017 cites W2036995683 @default.
- W4381893017 cites W2043856858 @default.
- W4381893017 cites W2048678109 @default.
- W4381893017 cites W2048994607 @default.
- W4381893017 cites W2049362524 @default.
- W4381893017 cites W2053791813 @default.
- W4381893017 cites W2056222380 @default.
- W4381893017 cites W2056306222 @default.
- W4381893017 cites W2061049714 @default.
- W4381893017 cites W2066371972 @default.
- W4381893017 cites W2079756038 @default.
- W4381893017 cites W2079999656 @default.
- W4381893017 cites W2083340704 @default.
- W4381893017 cites W2087646012 @default.
- W4381893017 cites W2092036624 @default.
- W4381893017 cites W2101579411 @default.
- W4381893017 cites W2101679570 @default.
- W4381893017 cites W2112595887 @default.
- W4381893017 cites W2122465033 @default.
- W4381893017 cites W2122620029 @default.
- W4381893017 cites W2127394968 @default.
- W4381893017 cites W2129338773 @default.
- W4381893017 cites W2133406830 @default.
- W4381893017 cites W2137681938 @default.
- W4381893017 cites W2138647063 @default.
- W4381893017 cites W2145842706 @default.
- W4381893017 cites W2148733641 @default.
- W4381893017 cites W2148972157 @default.
- W4381893017 cites W2156880617 @default.
- W4381893017 cites W2157703492 @default.
- W4381893017 cites W2158958901 @default.
- W4381893017 cites W2164487353 @default.
- W4381893017 cites W2170325085 @default.
- W4381893017 cites W2184391500 @default.
- W4381893017 cites W2334157044 @default.
- W4381893017 cites W2342358015 @default.
- W4381893017 cites W2410306399 @default.
- W4381893017 cites W2419140434 @default.
- W4381893017 cites W2528133039 @default.
- W4381893017 cites W2596052773 @default.
- W4381893017 cites W2596064219 @default.
- W4381893017 cites W2621269038 @default.
- W4381893017 cites W2763462375 @default.
- W4381893017 cites W2768202893 @default.
- W4381893017 cites W2777537279 @default.
- W4381893017 cites W2800987064 @default.
- W4381893017 cites W2809438292 @default.
- W4381893017 cites W2890296641 @default.
- W4381893017 cites W2907476149 @default.
- W4381893017 cites W2911426599 @default.
- W4381893017 cites W2915706892 @default.
- W4381893017 cites W2945657384 @default.
- W4381893017 cites W2979169072 @default.
- W4381893017 cites W2989877500 @default.
- W4381893017 cites W2992033677 @default.
- W4381893017 cites W3119554079 @default.
- W4381893017 cites W3133808435 @default.
- W4381893017 cites W3167425095 @default.
- W4381893017 cites W3185924723 @default.
- W4381893017 cites W4206373129 @default.
- W4381893017 cites W4220891964 @default.