FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4381996929> ?p ?o ?g. }

• W4381996929 abstract "Twenty-two atypical enteroaggregative Escherichia coli isolates from a previous epidemiological study harboring EAEC virulence genes were examined for their adhesion properties. Nine strains showed a typical aggregative adherence (AA) pattern, while 13 strains showed variant AA, such as AA with lined up cells characteristic of the chain-like adhesion (CLA) and AA mainly to HeLa cells characteristic of the diffuse adherence (DA). The aggregative forming pilus (AFP) genes afpA2 and afpR were detected only in strain Q015B, which exhibited an AA/DA pattern. Using Tn5-based transposon mutagenesis on Q015B strain, we identified a 5517-bp open reading frame (ORF) encoding a predicted 1838-amino-acid polypeptide that is genetically related to a putative filamentous hemagglutinin identified in E. coli strain 7-233-03_S3_C2. Therefore, the ORF was named orfHA . The regions flanking orfHA were sequenced and two ORFs were found; upstream, an ORF that encodes a 603-amino-acid polypeptide with 99% identity to hemolysin secretion/activation proteins of the ShlB/FhaC/HecB family, and downstream, another ORF, which encodes a 632-amino-acid polypeptide with 72% identity to the glycosyltransferase EtpC. An orfHA mutant (Q015BΔ orfHA ) was constructed from strain Q015B. Q015BΔ orfHA strain did not adhere to HeLa cells, whereas Q015BΔ orfHA transformed with a pACYC184 plasmid carrying orfHA restored the AA/DA phenotype of strain Q015B. Furthermore, the Q015Δ orfHA mutant had a marked effect on the ability of strain Q015B to kill the larvae of Galleria mellonella . Our results suggest that the AA/DA pattern of strain Q015B is mediated by a hemagglutinin-associated protein which also contributes to its virulence in the G. mellonella model." @default.
• W4381996929 created "2023-06-26" @default.
• W4381996929 creator A5005683415 @default.
• W4381996929 creator A5008239869 @default.
• W4381996929 creator A5009224654 @default.
• W4381996929 creator A5046408380 @default.
• W4381996929 creator A5086575985 @default.
• W4381996929 creator A5088420133 @default.
• W4381996929 date "2023-06-23" @default.
• W4381996929 modified "2023-09-30" @default.
• W4381996929 title "Transposon mutagenesis of atypical enteroaggregative Escherichia coli reveals a hemagglutinin-associated protein that mediates cell adhesion and contributes to the Galleria mellonella virulence" @default.
• W4381996929 cites W1894766918 @default.
• W4381996929 cites W1984302322 @default.
• W4381996929 cites W1997749635 @default.
• W4381996929 cites W2008307269 @default.
• W4381996929 cites W2025957152 @default.
• W4381996929 cites W2038900305 @default.
• W4381996929 cites W2041721199 @default.
• W4381996929 cites W2059027572 @default.
• W4381996929 cites W2059820723 @default.
• W4381996929 cites W2077721700 @default.
• W4381996929 cites W2089085698 @default.
• W4381996929 cites W2089340836 @default.
• W4381996929 cites W2099948471 @default.
• W4381996929 cites W2101628028 @default.
• W4381996929 cites W2109428580 @default.
• W4381996929 cites W2116137883 @default.
• W4381996929 cites W2118606775 @default.
• W4381996929 cites W2120109350 @default.
• W4381996929 cites W2151932607 @default.
• W4381996929 cites W2152012389 @default.
• W4381996929 cites W2154421450 @default.
• W4381996929 cites W2160344932 @default.
• W4381996929 cites W2161414686 @default.
• W4381996929 cites W2162539208 @default.
• W4381996929 cites W2164410631 @default.
• W4381996929 cites W2187419934 @default.
• W4381996929 cites W2499758600 @default.
• W4381996929 cites W2555651139 @default.
• W4381996929 cites W2902153911 @default.
• W4381996929 cites W2963843582 @default.
• W4381996929 cites W3020800828 @default.
• W4381996929 cites W4200037691 @default.
• W4381996929 cites W4292680380 @default.
• W4381996929 cites W4308595113 @default.
• W4381996929 doi "https://doi.org/10.3389/fcimb.2023.1166158" @default.
• W4381996929 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37424788" @default.
• W4381996929 hasPublicationYear "2023" @default.
• W4381996929 type Work @default.
• W4381996929 citedByCount "0" @default.
• W4381996929 crossrefType "journal-article" @default.
• W4381996929 hasAuthorship W4381996929A5005683415 @default.
• W4381996929 hasAuthorship W4381996929A5008239869 @default.
• W4381996929 hasAuthorship W4381996929A5009224654 @default.
• W4381996929 hasAuthorship W4381996929A5046408380 @default.
• W4381996929 hasAuthorship W4381996929A5086575985 @default.
• W4381996929 hasAuthorship W4381996929A5088420133 @default.
• W4381996929 hasBestOaLocation W43819969291 @default.
• W4381996929 hasConcept C104317684 @default.
• W4381996929 hasConcept C143065580 @default.
• W4381996929 hasConcept C153911025 @default.
• W4381996929 hasConcept C16318435 @default.
• W4381996929 hasConcept C167625842 @default.
• W4381996929 hasConcept C174832134 @default.
• W4381996929 hasConcept C22744801 @default.
• W4381996929 hasConcept C2777587559 @default.
• W4381996929 hasConcept C2778692840 @default.
• W4381996929 hasConcept C2778910516 @default.
• W4381996929 hasConcept C2779032433 @default.
• W4381996929 hasConcept C2780530800 @default.
• W4381996929 hasConcept C28693550 @default.
• W4381996929 hasConcept C47289529 @default.
• W4381996929 hasConcept C4918238 @default.
• W4381996929 hasConcept C54355233 @default.
• W4381996929 hasConcept C547475151 @default.
• W4381996929 hasConcept C60987743 @default.
• W4381996929 hasConcept C86803240 @default.
• W4381996929 hasConcept C89423630 @default.
• W4381996929 hasConceptScore W4381996929C104317684 @default.
• W4381996929 hasConceptScore W4381996929C143065580 @default.
• W4381996929 hasConceptScore W4381996929C153911025 @default.
• W4381996929 hasConceptScore W4381996929C16318435 @default.
• W4381996929 hasConceptScore W4381996929C167625842 @default.
• W4381996929 hasConceptScore W4381996929C174832134 @default.
• W4381996929 hasConceptScore W4381996929C22744801 @default.
• W4381996929 hasConceptScore W4381996929C2777587559 @default.
• W4381996929 hasConceptScore W4381996929C2778692840 @default.
• W4381996929 hasConceptScore W4381996929C2778910516 @default.
• W4381996929 hasConceptScore W4381996929C2779032433 @default.
• W4381996929 hasConceptScore W4381996929C2780530800 @default.
• W4381996929 hasConceptScore W4381996929C28693550 @default.
• W4381996929 hasConceptScore W4381996929C47289529 @default.
• W4381996929 hasConceptScore W4381996929C4918238 @default.
• W4381996929 hasConceptScore W4381996929C54355233 @default.
• W4381996929 hasConceptScore W4381996929C547475151 @default.
• W4381996929 hasConceptScore W4381996929C60987743 @default.
• W4381996929 hasConceptScore W4381996929C86803240 @default.
• W4381996929 hasConceptScore W4381996929C89423630 @default.
• W4381996929 hasLocation W43819969291 @default.
• W4381996929 hasLocation W43819969292 @default.

• next