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- W4382010796 abstract "Abstract This study aimed to investigate the regeneration of epithelial cells in the long‐term observation of ureter reconstruction by excising the demucosalized ileum. First, 8 Beagle dogs were anesthetized and the abdominal cavity was inspected for abnormalities via an abdominal incision. The right kidney and ureter were subsequently separated, and the ureter was severed from its connection to the renal pelvis and bladder and ligated distally. The 10–15 cm of ileum was used to reconstruct the ureter. The biopsies of the proximal, middle, and distal reconstructed ureter (neo‐ureter) were collected at the first, third, fifth, and sixth month postoperatively. The regeneration of ileal mucosa at the first, third, fifth, and sixth month was observed by hematoxylin–eosin (HE) staining and immunofluorescence staining cytokeratin 18 (CK18). HE staining results showed irregular cytoarchitecture, severe nuclear consolidation, and inflammatory infiltration in the proximal, middle, and distal neo‐ureters of dogs at the first month after ureteral reconstruction. With longer follow‐up, the injuries of the proximal, middle, and distal neo‐ureters were alleviated at the third, fifth, and sixth month after surgery. The expression of CK18 was higher in the middle neo‐ureters than that in the proximal and distal neo‐ureters at different time points after ureteral reconstruction and decreased with time. In summary, the present study demonstrated that demucosalized ileum was feasible for ureteral reconstructive surgery with satisfying prognostic effects." @default.
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- W4382010796 date "2023-06-26" @default.
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- W4382010796 title "Feasibility study on epithelial cells regeneration after excising the demucosalized ileum for ureteral reconstructive surgery" @default.
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- W4382010796 doi "https://doi.org/10.1096/fj.202201148r" @default.
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