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• W4382011023 endingPage "1872" @default.
• W4382011023 startingPage "1860" @default.
• W4382011023 abstract "Summary The ability of plant somatic cells to dedifferentiate, form somatic embryos and regenerate whole plants in vitro has been harnessed for both clonal propagation and as a key component of plant genetic engineering systems. Embryogenic culture response is significantly limited, however, by plant genotype in most species. This impedes advancements in both plant transformation‐based functional genomics research and crop improvement efforts. We utilized natural variation among maize inbred lines to genetically map somatic embryo generation potential in tissue culture and identify candidate genes underlying totipotency. Using a series of maize lines derived from crosses involving the culturable parent A188 and the non‐responsive parent B73, we identified a region on chromosome 3 associated with embryogenic culture response and focused on three candidate genes within the region based on genetic position and expression pattern. Two candidate genes showed no effect when ectopically expressed in B73, but the gene Wox2a was found to induce somatic embryogenesis and embryogenic callus proliferation. Transgenic B73 cells with strong constitutive expression of the B73 and A188 coding sequences of Wox2a were found to produce somatic embryos at similar frequencies, demonstrating that sufficient expression of either allele could rescue the embryogenic culture phenotype. Transgenic B73 plants were regenerated from the somatic embryos without chemical selection and no pleiotropic effects were observed in the Wox2a overexpression lines in the regenerated T0 plants or in the two independent events which produced T1 progeny. In addition to linking natural variation in tissue culture response to Wox2a , our data support the utility of Wox2a in enabling transformation of recalcitrant genotypes." @default.
• W4382011023 created "2023-06-27" @default.
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• W4382011023 creator A5085218579 @default.
• W4382011023 date "2023-06-26" @default.
• W4382011023 modified "2023-10-14" @default.
• W4382011023 title "A key to totipotency: <i>Wuschel‐like homeobox 2a</i> unlocks embryogenic culture response in maize (<i>Zea mays</i> L.)" @default.
• W4382011023 cites W1570155501 @default.
• W4382011023 cites W1968937403 @default.
• W4382011023 cites W1975012188 @default.
• W4382011023 cites W1982529946 @default.
• W4382011023 cites W1995551795 @default.
• W4382011023 cites W1995904887 @default.
• W4382011023 cites W1996272526 @default.
• W4382011023 cites W1998708845 @default.
• W4382011023 cites W2003652867 @default.
• W4382011023 cites W2011804618 @default.
• W4382011023 cites W2013917944 @default.
• W4382011023 cites W2021707325 @default.
• W4382011023 cites W2028018505 @default.
• W4382011023 cites W2030984741 @default.
• W4382011023 cites W2031875240 @default.
• W4382011023 cites W2032662888 @default.
• W4382011023 cites W2033691105 @default.
• W4382011023 cites W2034490325 @default.
• W4382011023 cites W2037593399 @default.
• W4382011023 cites W2041511003 @default.
• W4382011023 cites W2047252887 @default.
• W4382011023 cites W2052027914 @default.
• W4382011023 cites W2055043387 @default.
• W4382011023 cites W2055995857 @default.
• W4382011023 cites W2061308466 @default.
• W4382011023 cites W2062732100 @default.
• W4382011023 cites W2066965237 @default.
• W4382011023 cites W2073936714 @default.
• W4382011023 cites W2089632100 @default.
• W4382011023 cites W2093048999 @default.
• W4382011023 cites W2131354461 @default.
• W4382011023 cites W2136487066 @default.
• W4382011023 cites W2138464000 @default.
• W4382011023 cites W2142882075 @default.
• W4382011023 cites W2146341019 @default.
• W4382011023 cites W2148750035 @default.
• W4382011023 cites W2154388475 @default.
• W4382011023 cites W2163621988 @default.
• W4382011023 cites W2165404505 @default.
• W4382011023 cites W2305127580 @default.
• W4382011023 cites W2518225655 @default.
• W4382011023 cites W2568479992 @default.
• W4382011023 cites W2586786447 @default.
• W4382011023 cites W2587096119 @default.
• W4382011023 cites W2731682345 @default.
• W4382011023 cites W2732418005 @default.
• W4382011023 cites W2760121488 @default.
• W4382011023 cites W27634554 @default.
• W4382011023 cites W2791112841 @default.
• W4382011023 cites W2800329321 @default.
• W4382011023 cites W2800948667 @default.
• W4382011023 cites W2801634803 @default.
• W4382011023 cites W2802115440 @default.
• W4382011023 cites W2802998545 @default.
• W4382011023 cites W2900423624 @default.
• W4382011023 cites W2943105048 @default.
• W4382011023 cites W2955784013 @default.
• W4382011023 cites W2988115887 @default.
• W4382011023 cites W2995711654 @default.
• W4382011023 cites W2998353632 @default.
• W4382011023 cites W3006233023 @default.
• W4382011023 cites W3091894549 @default.
• W4382011023 cites W3091935517 @default.
• W4382011023 cites W3156279678 @default.
• W4382011023 cites W3166995393 @default.
• W4382011023 cites W3169762547 @default.
• W4382011023 cites W3179894672 @default.
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• W4382011023 cites W41803340 @default.
• W4382011023 cites W4206394861 @default.
• W4382011023 cites W4206979352 @default.
• W4382011023 cites W4214714043 @default.
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• W4382011023 doi "https://doi.org/10.1111/pbi.14098" @default.
• W4382011023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37357571" @default.
• W4382011023 hasPublicationYear "2023" @default.
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