FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4382045134> ?p ?o ?g. }

• W4382045134 endingPage "104972" @default.
• W4382045134 startingPage "104972" @default.
• W4382045134 abstract "Borrelial pathogens are vector-borne etiological agents known to cause Lyme disease, relapsing fever, and Borrelia miyamotoi disease. These spirochetes each encode several surface-localized lipoproteins that bind components of the human complement system to evade host immunity. One borrelial lipoprotein, BBK32, protects the Lyme disease spirochete from complement-mediated attack via an alpha helical C-terminal domain that interacts directly with the initiating protease of the classical complement pathway, C1r. In addition, the Borrelia miyamotoi BBK32 orthologs FbpA and FbpB also inhibit C1r, albeit via distinct recognition mechanisms. The C1r-inhibitory activities of a third ortholog termed FbpC, which is found exclusively in relapsing fever-causing spirochetes, remains unknown. Here we report the crystal structure of the C-terminal domain of B. hermsii FbpC to a limiting resolution of 1.5 Å. We used surface plasmon resonance and assays of complement function to demonstrate that FbpC retains potent BBK32-like anti-complement activities. Based on the structure of FbpC, we hypothesized that conformational dynamics of the complement inhibitory domains of borrelial C1r inhibitors may differ. To test this, we utilized the crystal structures of the C-terminal domains of BBK32, FbpA, FbpB, and FbpC to carry out molecular dynamics simulations, which revealed borrelial C1r inhibitors adopt energetically favored open and closed states defined by two functionally critical regions. Taken together, these results advance our understanding of how protein dynamics contribute to the function of bacterial immune evasion proteins and reveals a surprising plasticity in the structures of borrelial C1r inhibitors." @default.
• W4382045134 created "2023-06-27" @default.
• W4382045134 creator A5014066546 @default.
• W4382045134 creator A5025455529 @default.
• W4382045134 creator A5028013690 @default.
• W4382045134 creator A5052096739 @default.
• W4382045134 creator A5067154316 @default.
• W4382045134 creator A5081532874 @default.
• W4382045134 date "2023-08-01" @default.
• W4382045134 modified "2023-10-18" @default.
• W4382045134 title "Conformational dynamics of complement protease C1r inhibitor proteins from Lyme disease– and relapsing fever–causing spirochetes" @default.
• W4382045134 cites W1031578623 @default.
• W4382045134 cites W1482971402 @default.
• W4382045134 cites W1553626906 @default.
• W4382045134 cites W1669487765 @default.
• W4382045134 cites W1908771231 @default.
• W4382045134 cites W1956624115 @default.
• W4382045134 cites W1968830926 @default.
• W4382045134 cites W1971447551 @default.
• W4382045134 cites W1974523598 @default.
• W4382045134 cites W1977332355 @default.
• W4382045134 cites W1981021420 @default.
• W4382045134 cites W1981747417 @default.
• W4382045134 cites W1985750892 @default.
• W4382045134 cites W1997310186 @default.
• W4382045134 cites W2009356149 @default.
• W4382045134 cites W2010717419 @default.
• W4382045134 cites W2035266068 @default.
• W4382045134 cites W2035687084 @default.
• W4382045134 cites W2040232982 @default.
• W4382045134 cites W2041046009 @default.
• W4382045134 cites W2047538874 @default.
• W4382045134 cites W2049235801 @default.
• W4382045134 cites W2054115369 @default.
• W4382045134 cites W2055530511 @default.
• W4382045134 cites W2058078276 @default.
• W4382045134 cites W2065283382 @default.
• W4382045134 cites W2068128475 @default.
• W4382045134 cites W2081693079 @default.
• W4382045134 cites W2088906583 @default.
• W4382045134 cites W2095593044 @default.
• W4382045134 cites W2096228560 @default.
• W4382045134 cites W2101766243 @default.
• W4382045134 cites W2116073343 @default.
• W4382045134 cites W2123234828 @default.
• W4382045134 cites W2124383454 @default.
• W4382045134 cites W2125304058 @default.
• W4382045134 cites W2127322768 @default.
• W4382045134 cites W2128572087 @default.
• W4382045134 cites W2140448562 @default.
• W4382045134 cites W2154319440 @default.
• W4382045134 cites W2158191984 @default.
• W4382045134 cites W2159626137 @default.
• W4382045134 cites W2160451368 @default.
• W4382045134 cites W2161276511 @default.
• W4382045134 cites W2163713360 @default.
• W4382045134 cites W2169788329 @default.
• W4382045134 cites W2171268876 @default.
• W4382045134 cites W2190279855 @default.
• W4382045134 cites W2267745420 @default.
• W4382045134 cites W2436818885 @default.
• W4382045134 cites W2567086022 @default.
• W4382045134 cites W2569726227 @default.
• W4382045134 cites W2574359549 @default.
• W4382045134 cites W2577507040 @default.
• W4382045134 cites W2595045044 @default.
• W4382045134 cites W2601292926 @default.
• W4382045134 cites W2608236585 @default.
• W4382045134 cites W2612959023 @default.
• W4382045134 cites W2613266586 @default.
• W4382045134 cites W2763454568 @default.
• W4382045134 cites W2777260063 @default.
• W4382045134 cites W2892113269 @default.
• W4382045134 cites W2923645507 @default.
• W4382045134 cites W2926953440 @default.
• W4382045134 cites W2983303092 @default.
• W4382045134 cites W2990483783 @default.
• W4382045134 cites W3020601977 @default.
• W4382045134 cites W3026890009 @default.
• W4382045134 cites W3030988365 @default.
• W4382045134 cites W3045011089 @default.
• W4382045134 cites W3081938587 @default.
• W4382045134 cites W3127895000 @default.
• W4382045134 cites W3132760959 @default.
• W4382045134 cites W3134906143 @default.
• W4382045134 cites W3135881738 @default.
• W4382045134 cites W3177828909 @default.
• W4382045134 cites W3213333529 @default.
• W4382045134 cites W4281790889 @default.
• W4382045134 cites W4281896917 @default.
• W4382045134 cites W4290649235 @default.
• W4382045134 cites W4319439760 @default.
• W4382045134 doi "https://doi.org/10.1016/j.jbc.2023.104972" @default.
• W4382045134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37380082" @default.
• W4382045134 hasPublicationYear "2023" @default.
• W4382045134 type Work @default.
• W4382045134 citedByCount "0" @default.
• W4382045134 crossrefType "journal-article" @default.

• next