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• W4382048251 abstract "Abstract Background Chronic disease states can share inflammation as an underlying pathology. Macrophages are associated with chronic inflammation; in general, M1 phenotype macrophage activity inhibits cell proliferation and causes tissue damage whilst M2 macrophages promote proliferation and tissue repair. Human mesenchymal stem/stromal cells (hMSCs) are of particular interest for immunoregulatory diseases, through their ability to suppress T cell proliferation. Methods The application of a physiological oxygen environment to hMSC cultures modulates their gene expression and results in an altered secretome. Consequently, physiological, 2% O 2 hMSC SFCM was assessed in comparison to air oxygen (21% O 2 ) produced SFCM and applied to activated THP-1 cells in 10% and 21% O 2 to assess changes in macrophage differentiation. Results SFCM significantly suppressed proliferation and metabolic activity whilst promoting cell adherence compared to serum-free media (SFM) controls ( p < 0.001). Terminal differentiation phenotype varied in an oxygen-dependent manner, with 21% air oxygen SFCM leading to an increased M1:M2 macrophage differentiation ratio, with cells displaying distinct pancake-like morphology, increased TNFa secretion (21% O 2 = 922 ± 5 pg/mL, and 10% O 2 = 373 ± 2 pg/mL) and CD197 expression (21% = 69.1%±6.4, and 10% = 18.6 ± 0.4%). In contrast, physiological oxygen SFCM led to the production of a greater M2:M1 macrophage differentiation ratio; displaying an elongated spindle shape, increased IL-10 secretion (21% O 2 = 3780 ± 560 pg/mL, and 10% O 2 = 9115 ± 889), and CD3 and CD14 expression. SFCM collected under 2% O 2 suppressed pro-inflammatory transcriptional profiles through downregulation of TNFa, IL-1B, and IL-12B compared to 21% O 2 collected SFCM. Conclusion Collectively, SFCM induced terminal macrophage differentiation in an oxygen-dependent manner. This effect was not completely replicated by the application of isolated candidate anti-inflammatory cytokines (IL-4, IL-10, IL-13 or TGFb) or a combinational cytomix, highlighting the complex effects of conditioned media products and further work needed to determine mechanism of action." @default.
• W4382048251 created "2023-06-27" @default.
• W4382048251 creator A5030963147 @default.
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• W4382048251 date "2023-06-26" @default.
• W4382048251 modified "2023-09-26" @default.
• W4382048251 title "The Human Mesenchymal Stem Cell Secretome Directs Macrophage Differentiation in an Oxygen-Dependent Manner" @default.
• W4382048251 cites W1768846296 @default.
• W4382048251 cites W1918028620 @default.
• W4382048251 cites W1968159361 @default.
• W4382048251 cites W1972879829 @default.
• W4382048251 cites W1980285125 @default.
• W4382048251 cites W1980916386 @default.
• W4382048251 cites W1982408656 @default.
• W4382048251 cites W1991397191 @default.
• W4382048251 cites W1991460349 @default.
• W4382048251 cites W1996526080 @default.
• W4382048251 cites W2005408022 @default.
• W4382048251 cites W2008214282 @default.
• W4382048251 cites W2010993726 @default.
• W4382048251 cites W2013980575 @default.
• W4382048251 cites W2017720509 @default.
• W4382048251 cites W2021551978 @default.
• W4382048251 cites W2022194525 @default.
• W4382048251 cites W2023122744 @default.
• W4382048251 cites W2032234779 @default.
• W4382048251 cites W2036061724 @default.
• W4382048251 cites W2038775969 @default.
• W4382048251 cites W2041552691 @default.
• W4382048251 cites W2041999489 @default.
• W4382048251 cites W2043058475 @default.
• W4382048251 cites W2043263890 @default.
• W4382048251 cites W2043457504 @default.
• W4382048251 cites W2054472394 @default.
• W4382048251 cites W2054719244 @default.
• W4382048251 cites W2069399162 @default.
• W4382048251 cites W2076491580 @default.
• W4382048251 cites W2084037323 @default.
• W4382048251 cites W2093705034 @default.
• W4382048251 cites W2094131843 @default.
• W4382048251 cites W2102623170 @default.
• W4382048251 cites W2107551122 @default.
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• W4382048251 cites W2111727066 @default.
• W4382048251 cites W2111913995 @default.
• W4382048251 cites W2112527416 @default.
• W4382048251 cites W2116738903 @default.
• W4382048251 cites W2131630407 @default.
• W4382048251 cites W2138946470 @default.
• W4382048251 cites W2139973886 @default.
• W4382048251 cites W2147425799 @default.
• W4382048251 cites W2152532651 @default.
• W4382048251 cites W2154713489 @default.
• W4382048251 cites W2155338282 @default.
• W4382048251 cites W2155945484 @default.
• W4382048251 cites W2159560463 @default.
• W4382048251 cites W2161074777 @default.
• W4382048251 cites W2337861353 @default.
• W4382048251 cites W2415420071 @default.
• W4382048251 cites W2466464742 @default.
• W4382048251 cites W2478193138 @default.
• W4382048251 cites W2489622437 @default.
• W4382048251 cites W2530424978 @default.
• W4382048251 cites W2586312403 @default.
• W4382048251 cites W2610297465 @default.
• W4382048251 cites W2730923948 @default.
• W4382048251 cites W2747004888 @default.
• W4382048251 cites W2771472591 @default.
• W4382048251 cites W2776702021 @default.
• W4382048251 cites W2786954262 @default.
• W4382048251 cites W2791533291 @default.
• W4382048251 cites W2792366526 @default.
• W4382048251 cites W2792895473 @default.
• W4382048251 cites W2806376977 @default.
• W4382048251 cites W2807906301 @default.
• W4382048251 cites W2887516352 @default.
• W4382048251 cites W2897996477 @default.
• W4382048251 cites W2930670575 @default.
• W4382048251 cites W2945777288 @default.
• W4382048251 cites W2993908729 @default.
• W4382048251 cites W3015515478 @default.
• W4382048251 cites W3037592572 @default.
• W4382048251 cites W3087797540 @default.
• W4382048251 cites W3118522749 @default.
• W4382048251 cites W3127853020 @default.
• W4382048251 cites W3130038046 @default.
• W4382048251 cites W3208931086 @default.
• W4382048251 cites W3213753238 @default.
• W4382048251 cites W4220797111 @default.
• W4382048251 cites W4254672925 @default.
• W4382048251 cites W4294243564 @default.
• W4382048251 cites W4308523010 @default.
• W4382048251 doi "https://doi.org/10.21203/rs.3.rs-3006436/v1" @default.
• W4382048251 hasPublicationYear "2023" @default.
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