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• W4382051504 endingPage "107030" @default.
• W4382051504 startingPage "107030" @default.
• W4382051504 abstract "This is the first study to optimize a long distance transportation protocol for cryopreservation of Beetal buck (Capra hircus) spermatozoa by holding at different cooling temperatures vis-a-vis, 18 °C (T1), 12 °C (T2), and 4 °C (T3) for 24 h in non-glycerolated (step 1) cryodiluents compared to control (4 °C). Sperm quality was assessed through CASA motion (progressive motility, PM, %) and velocity (rapid velocity, RV, % and medium velocity, MV, %) and kinematics (average path velocity, VAP, μm/s; straight line velocity, VSL, μm/s; linearity, LIN, VSL/VCL, %; straightness, STR, VSL/VAP,%; curvilinear velocity, VCL, μm/s; amplitude of lateral head displacement, ALH, μm and beat cross frequency, BCF, Hz), and assays including supra–vital plasma membrane integrity, SV–PMI, %; mitochondrial transmembrane potential, MMP, %; viable and intact acrosome, V–IACR, % and DNA integrity, DNA–I,%. The cryopreservation stages were post–dilution, non-glycerated; pre-cooling, glycerolated; pre–freezing; and post–thawing. Significantly greater outcomes for PM, RV, VAP, VSL, STR, LIN, VCL, BCF, SV–PMI, MMP, and V–IACR (except DNA-I) assays in control and T1 were observed compared to T2 and T3, respectively at all cryopreservation stages. Pearson’s positive correlation was observed between all CASA variables and assays. Significantly higher fertilization rates (%) were observed in control (71.43) and T1 (65.71) as compared to T2 (44.44) and T3 (45.16), respectively. In conclusion, the study optimized the long distance transportation protocol by holding the buck spermatozoa at control and T1 cooling temperatures and thereby retaining their cryopreserved quality and in vivo fertilization potential." @default.
• W4382051504 created "2023-06-27" @default.
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• W4382051504 date "2023-09-01" @default.
• W4382051504 modified "2023-09-27" @default.
• W4382051504 title "Cryopreservation protocol resolving the temperature challenges of long-distance transportation of Beetal buck (Capra hircus) sperm" @default.
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• W4382051504 doi "https://doi.org/10.1016/j.smallrumres.2023.107030" @default.
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