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- W4382141018 abstract "Adiponectin, the most abundant protein secreted by white adipose tissue, is known for its involvement in obesity-related disorders such as insulin resistance, type 2 diabetes mellitus and atherosclerosis. Besides its well-known insulin-sensitizing, anti-inflammatory and antiatherosclerotic properties, accumulating evidence suggests that adiponectin may also have anticancer properties and be cardioprotective. The aim of this study is to investigate electrochemical behaviour of adiponectin at carbon paste electrode using adsorptive transfer stripping square wave voltammetry (AdTS SWV). To obtain the most sensitive analytical tool for determination of protein of interest, the time of accumulation on the surface of carbon paste electrode (CPE) and pH of supporting electrolyte (0.2 M phosphate buffer) was optimized. Under the optimized conditions (time of accumulation: 300 s, supporting electrolyte: 0.2 M phosphate buffer pH 6.98), the detection limit (3 signal/noise, S/N) for adiponectin was calculated as 50 pg/ml (250 fg in 5 μl). To investigate the collagen-adiponectin interactions, studying of electroactivity of collagen in CPE was needed. Collagen-modified CPE was prepared simply by mixing a desired quantity of collagen into a conventionally prepared CPE composed of graphite powder and mineral oil. The detection limit (3 signal/noise, S/N) for collagen was calculated as 100 ng/ml. Further, we tested mutual interactions between adiponectin and collagen and found the optimal time of incubation of these proteins as well suggested a possible scheme of their interaction." @default.
- W4382141018 created "2023-06-27" @default.
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- W4382141018 date "2012-01-01" @default.
- W4382141018 modified "2023-10-03" @default.
- W4382141018 title "Voltammetry of Adiponectin and its Interactions with Collagen on a Carbon Paste Electrode at Femtogram Level" @default.
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- W4382141018 doi "https://doi.org/10.1016/s1452-3981(23)13316-5" @default.
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