Matches in SemOpenAlex for { <https://semopenalex.org/work/W4382203606> ?p ?o ?g. }
- W4382203606 endingPage "696" @default.
- W4382203606 startingPage "682" @default.
- W4382203606 abstract "Neurodegeneration and cerebrovascular disease share an underlying microvascular dysfunction that may be remedied by selective transgene delivery. To date, limited options exist in which cellular components of the brain vasculature can be effectively targeted by viral vector therapeutics. In this study, we characterize the first engineered adeno-associated virus (AAV) capsid mediating high transduction of cerebral vascular pericytes and smooth muscle cells (SMCs). We performed two rounds of in vivo selection with an AAV capsid scaffold displaying a heptamer peptide library to isolate capsids that traffic to the brain after intravenous delivery. One identified capsid, termed AAV-PR, demonstrated high transduction of the brain vasculature, in contrast to the parental capsid, AAV9, which transduces mainly neurons and astrocytes. Further analysis using tissue clearing, volumetric rendering, and colocalization revealed that AAV-PR enabled high transduction of cerebral pericytes located on small-caliber vessels and SMCs in the larger arterioles and penetrating pial arteries. Analysis of tissues in the periphery indicated that AAV-PR also transduced SMCs in large vessels associated with the systemic vasculature. AAV-PR was also able to transduce primary human brain pericytes with higher efficiency than AAV9. Compared with previously published AAV capsids tropisms, AAV-PR represents the first capsid to allow for effective transduction of brain pericytes and SMCs and offers the possibility of genetically modulating these cell types in the context of neurodegeneration and other neurological diseases." @default.
- W4382203606 created "2023-06-28" @default.
- W4382203606 creator A5011401538 @default.
- W4382203606 creator A5012728368 @default.
- W4382203606 creator A5021240445 @default.
- W4382203606 creator A5029064436 @default.
- W4382203606 creator A5033170987 @default.
- W4382203606 creator A5043238026 @default.
- W4382203606 creator A5045468827 @default.
- W4382203606 creator A5054468518 @default.
- W4382203606 creator A5055812252 @default.
- W4382203606 creator A5064090998 @default.
- W4382203606 creator A5064371575 @default.
- W4382203606 creator A5074993694 @default.
- W4382203606 creator A5077624190 @default.
- W4382203606 creator A5082242705 @default.
- W4382203606 creator A5084190685 @default.
- W4382203606 creator A5089297674 @default.
- W4382203606 date "2023-08-01" @default.
- W4382203606 modified "2023-10-15" @default.
- W4382203606 title "An Engineered Adeno-Associated Virus Capsid Mediates Efficient Transduction of Pericytes and Smooth Muscle Cells of the Brain Vasculature" @default.
- W4382203606 cites W1973418971 @default.
- W4382203606 cites W1981950767 @default.
- W4382203606 cites W1986263030 @default.
- W4382203606 cites W1988213225 @default.
- W4382203606 cites W2011494892 @default.
- W4382203606 cites W2025640763 @default.
- W4382203606 cites W2051642566 @default.
- W4382203606 cites W2051889170 @default.
- W4382203606 cites W2079177429 @default.
- W4382203606 cites W2109781922 @default.
- W4382203606 cites W2126232130 @default.
- W4382203606 cites W2139023977 @default.
- W4382203606 cites W2142177110 @default.
- W4382203606 cites W2166739815 @default.
- W4382203606 cites W2200746626 @default.
- W4382203606 cites W2329803151 @default.
- W4382203606 cites W2330487030 @default.
- W4382203606 cites W2339068621 @default.
- W4382203606 cites W2617054174 @default.
- W4382203606 cites W2785806346 @default.
- W4382203606 cites W2886442693 @default.
- W4382203606 cites W2890224686 @default.
- W4382203606 cites W2964541844 @default.
- W4382203606 cites W2982053609 @default.
- W4382203606 cites W3008199088 @default.
- W4382203606 cites W3016724860 @default.
- W4382203606 cites W3104571446 @default.
- W4382203606 cites W3115131581 @default.
- W4382203606 cites W3165519430 @default.
- W4382203606 cites W3182324325 @default.
- W4382203606 cites W3217304922 @default.
- W4382203606 cites W4210331631 @default.
- W4382203606 cites W4280523474 @default.
- W4382203606 cites W4285025887 @default.
- W4382203606 cites W4309046731 @default.
- W4382203606 doi "https://doi.org/10.1089/hum.2022.211" @default.
- W4382203606 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37376759" @default.
- W4382203606 hasPublicationYear "2023" @default.
- W4382203606 type Work @default.
- W4382203606 citedByCount "0" @default.
- W4382203606 crossrefType "journal-article" @default.
- W4382203606 hasAuthorship W4382203606A5011401538 @default.
- W4382203606 hasAuthorship W4382203606A5012728368 @default.
- W4382203606 hasAuthorship W4382203606A5021240445 @default.
- W4382203606 hasAuthorship W4382203606A5029064436 @default.
- W4382203606 hasAuthorship W4382203606A5033170987 @default.
- W4382203606 hasAuthorship W4382203606A5043238026 @default.
- W4382203606 hasAuthorship W4382203606A5045468827 @default.
- W4382203606 hasAuthorship W4382203606A5054468518 @default.
- W4382203606 hasAuthorship W4382203606A5055812252 @default.
- W4382203606 hasAuthorship W4382203606A5064090998 @default.
- W4382203606 hasAuthorship W4382203606A5064371575 @default.
- W4382203606 hasAuthorship W4382203606A5074993694 @default.
- W4382203606 hasAuthorship W4382203606A5077624190 @default.
- W4382203606 hasAuthorship W4382203606A5082242705 @default.
- W4382203606 hasAuthorship W4382203606A5084190685 @default.
- W4382203606 hasAuthorship W4382203606A5089297674 @default.
- W4382203606 hasConcept C102230213 @default.
- W4382203606 hasConcept C104317684 @default.
- W4382203606 hasConcept C142724271 @default.
- W4382203606 hasConcept C15152581 @default.
- W4382203606 hasConcept C159047783 @default.
- W4382203606 hasConcept C169760540 @default.
- W4382203606 hasConcept C202878990 @default.
- W4382203606 hasConcept C2522874641 @default.
- W4382203606 hasConcept C2776925932 @default.
- W4382203606 hasConcept C2778107364 @default.
- W4382203606 hasConcept C2779134260 @default.
- W4382203606 hasConcept C40209533 @default.
- W4382203606 hasConcept C40767141 @default.
- W4382203606 hasConcept C54355233 @default.
- W4382203606 hasConcept C55493867 @default.
- W4382203606 hasConcept C71924100 @default.
- W4382203606 hasConcept C86803240 @default.
- W4382203606 hasConcept C92087593 @default.
- W4382203606 hasConcept C95444343 @default.
- W4382203606 hasConceptScore W4382203606C102230213 @default.