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- W4382343830 abstract "Synthetic red fluorescent protein (RFP) chromophores have emerged as valuable tools for biological imaging and therapeutic applications, but their application in the visualization of endogenous RNA G-quadruplexes (G4s) in living cells has been rarely reported so far. Here, by integrating the group of the excellent G4 dye ThT, we modulate RFP chromophores to create a novel fluorescent probe DEBIT with red emission. DEBIT selectively recognizes the G4 structure with the advantage of strong binding affinity, high selectivity, and excellent photostability. Using DEBIT as a fluorescent indicator, the real-time monitoring of RNA G4 in biological systems can be achieved. In summary, our work expands the application of synthetic RFP chromophores and provides an essential dye category to the classical G4 probes." @default.
- W4382343830 created "2023-06-29" @default.
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- W4382343830 date "2023-06-28" @default.
- W4382343830 modified "2023-10-17" @default.
- W4382343830 title "Engineering a Red Fluorescent Protein Chromophore for Visualization of RNA G-Quadruplexes" @default.
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- W4382343830 doi "https://doi.org/10.1021/acs.biochem.3c00149" @default.
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