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- W4382400145 abstract "This study aimed to the molecular identification of isolated bacteria from the soils of Likouala (Congo-Brazzaville) peat bog area. Counting and isolation were carried out on Mossel and TSB media enriched with petroleum and vegetable oil; the amplification and sequencing of 16S RNA genes by PCR and Artic Oxford Nanopore Technology. The results showed bacterial loads of (5.81 ± 1.08) × 104; (6.64 ± 1.94) × 104 et (8.56 ± 1.19) × 103 CFU/g on Mossel respectively for samples 1, 2 and 3 against (2.12 ± 4.1) × 108 et (8.15 ± 10.1) × 107 CFU/g respectively on TSB enriched with petroleum and vegetable oil exclusively with sample 2. The analysis of the 16S rRNA of the isolates gene made it possible, after PCR, agarose gel electrophoresis, sequencing and bioinformatics analysis, to identify eight (08) strains with similarities of 99 to 100% whose sequences genes placed in GenBank have made it possible to obtain accession numbers corresponding to: Bacillus thuringiensis strain (ON303633); Bacillus cereus strain (ON350770); Bacillus thuringiensis strain (ON350771); Bacillus thuringiensis strain (ON738723); Priestia megaterium strain (ON738719); Bacillus anthracis strain (ON738720); Bacillus subtilis (ON738721); Enterobacter sp (ON738722). The phylogenetic classification of strains was done and revealed two genuses which are Bacillus and Enterobacteriaceae." @default.
- W4382400145 created "2023-06-29" @default.
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- W4382400145 date "2023-01-01" @default.
- W4382400145 modified "2023-09-30" @default.
- W4382400145 title "Molecular Identification of Isolated Bacteria from Soils in Likouala Peat Bog Area, Republic of Congo" @default.
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- W4382400145 doi "https://doi.org/10.4236/ojss.2023.136011" @default.
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