FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4382403886> ?p ?o ?g. }

• W4382403886 abstract "Type II topoisomerases effect topological changes in DNA by cutting a single duplex, passing a second duplex through the break, and resealing the broken strand in an ATP-coupled reaction. Curiously, most type II topoisomerases (topos II, IV, and VI) catalyze DNA transformations that are energetically favorable, such as the removal of superhelical strain; why ATP is required for such reactions is unknown. Here, using human topoisomerase II β (hTOP2β) as a model, we show that the ATPase domains of the enzyme are not required for DNA strand passage, but that their loss leads to increased DNA nicking and double strand break formation by the enzyme. The unstructured C-terminal domains (CTDs) of hTOP2β strongly potentiate strand passage activity in the absence of the ATPase regions, as do cleavage-prone mutations that confer hypersensitivity to the chemotherapeutic agent etoposide. The presence of either the CTD or the mutations lead ATPase-less enzymes to promote even greater levels of DNA cleava in ge vitro , as well as in vivo . By contrast, the aberrant cleavage phenotypes of these topo II variants is significantly repressed when the ATPase domains are restored. Our findings are consistent with the proposal that type II topoisomerases acquired an ATPase function to maintain high levels of catalytic activity while minimizing inappropriate DNA damage." @default.
• W4382403886 created "2023-06-29" @default.
• W4382403886 creator A5022566853 @default.
• W4382403886 creator A5026107799 @default.
• W4382403886 creator A5039868655 @default.
• W4382403886 creator A5055294147 @default.
• W4382403886 creator A5072102696 @default.
• W4382403886 creator A5080912142 @default.
• W4382403886 date "2023-06-27" @default.
• W4382403886 modified "2023-10-18" @default.
• W4382403886 title "Using energy to go downhill – a genoprotective role for ATPase activity in DNA topoisomerase II" @default.
• W4382403886 cites W1491294973 @default.
• W4382403886 cites W1539052045 @default.
• W4382403886 cites W1546404670 @default.
• W4382403886 cites W1969656310 @default.
• W4382403886 cites W1971439989 @default.
• W4382403886 cites W1979700870 @default.
• W4382403886 cites W1980184916 @default.
• W4382403886 cites W1996007131 @default.
• W4382403886 cites W2008364078 @default.
• W4382403886 cites W2011223597 @default.
• W4382403886 cites W2012083162 @default.
• W4382403886 cites W2018468886 @default.
• W4382403886 cites W2021760534 @default.
• W4382403886 cites W2027611515 @default.
• W4382403886 cites W2030233737 @default.
• W4382403886 cites W2034319912 @default.
• W4382403886 cites W2038532304 @default.
• W4382403886 cites W2040188253 @default.
• W4382403886 cites W2050517163 @default.
• W4382403886 cites W2056367986 @default.
• W4382403886 cites W2058940094 @default.
• W4382403886 cites W2060268336 @default.
• W4382403886 cites W2061301880 @default.
• W4382403886 cites W2070231128 @default.
• W4382403886 cites W2072413382 @default.
• W4382403886 cites W2074651320 @default.
• W4382403886 cites W2080892050 @default.
• W4382403886 cites W2083921717 @default.
• W4382403886 cites W2091512138 @default.
• W4382403886 cites W2091690122 @default.
• W4382403886 cites W2092638438 @default.
• W4382403886 cites W2094476648 @default.
• W4382403886 cites W2097716081 @default.
• W4382403886 cites W2099540110 @default.
• W4382403886 cites W2123374199 @default.
• W4382403886 cites W2127960819 @default.
• W4382403886 cites W2142727309 @default.
• W4382403886 cites W2142775912 @default.
• W4382403886 cites W2157147749 @default.
• W4382403886 cites W2162764839 @default.
• W4382403886 cites W2163169974 @default.
• W4382403886 cites W2217631730 @default.
• W4382403886 cites W2745131378 @default.
• W4382403886 cites W3091885121 @default.
• W4382403886 cites W4308366968 @default.
• W4382403886 cites W4318964044 @default.
• W4382403886 doi "https://doi.org/10.1101/2023.06.27.546777" @default.
• W4382403886 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37425896" @default.
• W4382403886 hasPublicationYear "2023" @default.
• W4382403886 type Work @default.
• W4382403886 citedByCount "1" @default.
• W4382403886 countsByYear W43824038862023 @default.
• W4382403886 crossrefType "posted-content" @default.
• W4382403886 hasAuthorship W4382403886A5022566853 @default.
• W4382403886 hasAuthorship W4382403886A5026107799 @default.
• W4382403886 hasAuthorship W4382403886A5039868655 @default.
• W4382403886 hasAuthorship W4382403886A5055294147 @default.
• W4382403886 hasAuthorship W4382403886A5072102696 @default.
• W4382403886 hasAuthorship W4382403886A5080912142 @default.
• W4382403886 hasBestOaLocation W43824038861 @default.
• W4382403886 hasConcept C143425029 @default.
• W4382403886 hasConcept C147897179 @default.
• W4382403886 hasConcept C151730666 @default.
• W4382403886 hasConcept C153911025 @default.
• W4382403886 hasConcept C175156509 @default.
• W4382403886 hasConcept C181199279 @default.
• W4382403886 hasConcept C185592680 @default.
• W4382403886 hasConcept C23265538 @default.
• W4382403886 hasConcept C2776694085 @default.
• W4382403886 hasConcept C2778119113 @default.
• W4382403886 hasConcept C43369102 @default.
• W4382403886 hasConcept C54355233 @default.
• W4382403886 hasConcept C552990157 @default.
• W4382403886 hasConcept C55493867 @default.
• W4382403886 hasConcept C86803240 @default.
• W4382403886 hasConceptScore W4382403886C143425029 @default.
• W4382403886 hasConceptScore W4382403886C147897179 @default.
• W4382403886 hasConceptScore W4382403886C151730666 @default.
• W4382403886 hasConceptScore W4382403886C153911025 @default.
• W4382403886 hasConceptScore W4382403886C175156509 @default.
• W4382403886 hasConceptScore W4382403886C181199279 @default.
• W4382403886 hasConceptScore W4382403886C185592680 @default.
• W4382403886 hasConceptScore W4382403886C23265538 @default.
• W4382403886 hasConceptScore W4382403886C2776694085 @default.
• W4382403886 hasConceptScore W4382403886C2778119113 @default.
• W4382403886 hasConceptScore W4382403886C43369102 @default.
• W4382403886 hasConceptScore W4382403886C54355233 @default.
• W4382403886 hasConceptScore W4382403886C552990157 @default.
• W4382403886 hasConceptScore W4382403886C55493867 @default.

• next