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- W4382488305 abstract "The purpose of this study was to optimize the encapsulated formulation and freeze-drying conditions of Lactobacillus acidophilus KBL409 to improve shelf-life. The optimum freeze-drying conditions was 8% sucrose as a lyoprotectant at −30 °C. In addition, the free, alginate-encapsulated (Al-), and alginate/chitosan-encapsulated (Al/Chi-) L. acidophilus KBL409 with or without sucrose were prepared and evaluated for the simulated gastrointestinal (SGI) tract, moisture characteristics, and storage stability. The freeze-dried Al/Chi-cells with sucrose displayed the highest survival rate of 0.14% in the SGI tract (p < 0.05). The cells showed sigmoidal sorption isotherms and moisture content of 0.084 kg water/kg solid for Brunauer-Emmett-Teller-monolayer. At 4 °C/60% RH, the glass transition temperature was 12.55 °C, which allowed for 12-week period of glassy state and the highest storage viability of 22.35%. In conclusion, the viability of Lactobacillus acidophilus KBL409 faces distinct challenges for freeze-drying and storage, and encapsulation with lyoprotectant affects various stability and quality attributes of microorganisms." @default.
- W4382488305 created "2023-06-29" @default.
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- W4382488305 date "2023-06-01" @default.
- W4382488305 modified "2023-09-28" @default.
- W4382488305 title "Effects of lyoprotectant and encapsulated Lactobacillus acidophilus KBL409 on freeze-drying and storage stability" @default.
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- W4382488305 doi "https://doi.org/10.1016/j.lwt.2023.114846" @default.
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