FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4382501786> ?p ?o ?g. }

• W4382501786 endingPage "1440" @default.
• W4382501786 startingPage "1429" @default.
• W4382501786 abstract "BACKGROUND: Increasing evidence suggests that superoxide ions produced by NOX (nicotinamide adenine dinucleotide phosphate oxidases) mediate vascular effects of Ang II (angiotensin II) evoked by atherogenic diets. Here, we analyzed the mechanism by which NOX2 contributes to Ang II–induced ET-1 (endothelin 1) production in human microvascular endothelial cells. METHODS: The effects of high-fat diet were compared between WT (wild type) and Nox2 ( mouse NOX2 gene )-deficient mice. ET-1 production and NOX2 expression by human microvascular endothelial cells in vitro were analyzed by ELISA, reverse transcription quantitative polymerase chain reaction, electrophoretic mobility shift assay, promoter deletions, RNA interference, and pharmacological inhibition. Production of superoxide anions was visualized by fluorescent cell labeling. RESULTS: Feeding mice high-fat diet for 10 weeks increased cardiac expression and plasma levels of Ang II and ET-1 in WT but not in Nox2 -deficient animals. Exposure of human microvascular endothelial cells to Ang II resulted in increased ET-1 production, which could be blocked by silencing NOX2 ( human NOX2 gene ). Ang II promoted NOX2 expression through induction of the Oct-1 (human/mouse octamer binding transcription factor 1 protein) and activation of the NOX2 promoter region containing Oct-1–binding sites. Stimulation of NOX2 expression by Ang II was associated with increased production of superoxide anions. Inhibition of Oct-1 by small interfering RNA reduced Ang II–induced NOX2 expression and superoxide anion production, and neutralization of superoxide by SOD (superoxide dismutase) abolished Ang II–stimulated ET1 ( human ET-1 gene ) promoter activity, ET1 mRNA expression, and ET-1 release. CONCLUSIONS: Ang II may promote ET-1 production in the endothelium in response to atherogenic diets through a mechanism that involves the transcription factor Oct-1 and the increased formation of superoxide anions by NOX2." @default.
• W4382501786 created "2023-06-30" @default.
• W4382501786 creator A5006481103 @default.
• W4382501786 creator A5009553066 @default.
• W4382501786 creator A5010738468 @default.
• W4382501786 creator A5025109541 @default.
• W4382501786 creator A5034627046 @default.
• W4382501786 creator A5040501781 @default.
• W4382501786 creator A5040932572 @default.
• W4382501786 creator A5050238342 @default.
• W4382501786 creator A5055184252 @default.
• W4382501786 creator A5067215851 @default.
• W4382501786 creator A5076798343 @default.
• W4382501786 creator A5081891613 @default.
• W4382501786 creator A5092356050 @default.
• W4382501786 date "2023-08-01" @default.
• W4382501786 modified "2023-10-01" @default.
• W4382501786 title "Ang II Promotes ET-1 Production by Regulating NOX2 Activity Through Transcription Factor Oct-1" @default.
• W4382501786 cites W1966145991 @default.
• W4382501786 cites W1966774066 @default.
• W4382501786 cites W1971555177 @default.
• W4382501786 cites W1976555160 @default.
• W4382501786 cites W1980742012 @default.
• W4382501786 cites W1990111257 @default.
• W4382501786 cites W1991416826 @default.
• W4382501786 cites W1993010343 @default.
• W4382501786 cites W2000850056 @default.
• W4382501786 cites W2003200648 @default.
• W4382501786 cites W2011863986 @default.
• W4382501786 cites W2012358418 @default.
• W4382501786 cites W2020359290 @default.
• W4382501786 cites W2022844151 @default.
• W4382501786 cites W2023578446 @default.
• W4382501786 cites W2030923544 @default.
• W4382501786 cites W2031200353 @default.
• W4382501786 cites W2031607451 @default.
• W4382501786 cites W2033062120 @default.
• W4382501786 cites W2038091153 @default.
• W4382501786 cites W2046807579 @default.
• W4382501786 cites W2047457782 @default.
• W4382501786 cites W2082738807 @default.
• W4382501786 cites W2084871714 @default.
• W4382501786 cites W2085128107 @default.
• W4382501786 cites W2098576019 @default.
• W4382501786 cites W2104077271 @default.
• W4382501786 cites W2110182057 @default.
• W4382501786 cites W2110354274 @default.
• W4382501786 cites W2110940511 @default.
• W4382501786 cites W2111644571 @default.
• W4382501786 cites W2127773685 @default.
• W4382501786 cites W2129698177 @default.
• W4382501786 cites W2149408586 @default.
• W4382501786 cites W2157615046 @default.
• W4382501786 cites W2159785173 @default.
• W4382501786 cites W2266349963 @default.
• W4382501786 cites W2322654844 @default.
• W4382501786 cites W2330906137 @default.
• W4382501786 cites W2484838094 @default.
• W4382501786 cites W2592371738 @default.
• W4382501786 cites W2766752424 @default.
• W4382501786 cites W2805556916 @default.
• W4382501786 cites W2806295635 @default.
• W4382501786 cites W2897504384 @default.
• W4382501786 cites W2912238004 @default.
• W4382501786 cites W2954751444 @default.
• W4382501786 cites W2982095812 @default.
• W4382501786 cites W2983063065 @default.
• W4382501786 cites W3082919186 @default.
• W4382501786 cites W3094562382 @default.
• W4382501786 cites W3168173367 @default.
• W4382501786 cites W3201621510 @default.
• W4382501786 cites W4210343616 @default.
• W4382501786 cites W4296816736 @default.
• W4382501786 doi "https://doi.org/10.1161/atvbaha.122.318764" @default.
• W4382501786 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37381986" @default.
• W4382501786 hasPublicationYear "2023" @default.
• W4382501786 type Work @default.
• W4382501786 citedByCount "1" @default.
• W4382501786 countsByYear W43825017862023 @default.
• W4382501786 crossrefType "journal-article" @default.
• W4382501786 hasAuthorship W4382501786A5006481103 @default.
• W4382501786 hasAuthorship W4382501786A5009553066 @default.
• W4382501786 hasAuthorship W4382501786A5010738468 @default.
• W4382501786 hasAuthorship W4382501786A5025109541 @default.
• W4382501786 hasAuthorship W4382501786A5034627046 @default.
• W4382501786 hasAuthorship W4382501786A5040501781 @default.
• W4382501786 hasAuthorship W4382501786A5040932572 @default.
• W4382501786 hasAuthorship W4382501786A5050238342 @default.
• W4382501786 hasAuthorship W4382501786A5055184252 @default.
• W4382501786 hasAuthorship W4382501786A5067215851 @default.
• W4382501786 hasAuthorship W4382501786A5076798343 @default.
• W4382501786 hasAuthorship W4382501786A5081891613 @default.
• W4382501786 hasAuthorship W4382501786A5092356050 @default.
• W4382501786 hasConcept C104317684 @default.
• W4382501786 hasConcept C119056186 @default.
• W4382501786 hasConcept C150194340 @default.
• W4382501786 hasConcept C153911025 @default.
• W4382501786 hasConcept C170493617 @default.

• next