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- W4382645982 abstract "Abstract Spatial transcriptomic technologies are promising tools to reveal fine anatomical profiles of tissues. As for the methodologies based on barcoded probe arrays, improving the balance among probe barcoding complexity and cost, gene capture sensitivity, and spatial resolution can accelerate the spreading of spatial transcriptomic in basic science and clinical work. Here, based on miniaturized microfluidic and microarray technologies, we constructed a spatially cellular-level RNA-capture probe arrays. Owing to the predetermined and cost-effective probe fixation characteristics of the methodology, the consumable cost and fabrication time of the probe array can be reduced to $1.21/mm 2 and approximately 2 hours, and the preparation process does not rely on large precision instruments. Moreover, the efficiency of the transcript captured by the probe array is even comparable to conventional single-cell RNA sequencing. Based on this technology, we achieved the spatial transcriptome expression mapping and gained insight into spatial cell heterogeneity of the mouse hippocampus." @default.
- W4382645982 created "2023-07-01" @default.
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- W4382645982 date "2023-06-30" @default.
- W4382645982 modified "2023-09-25" @default.
- W4382645982 title "Well-ST-seq: cost-effective spatial transcriptomics at cellular level and high RNA capture efficiency" @default.
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- W4382645982 doi "https://doi.org/10.1101/2023.06.28.546974" @default.
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