Matches in SemOpenAlex for { <https://semopenalex.org/work/W4382700800> ?p ?o ?g. }
- W4382700800 endingPage "123810" @default.
- W4382700800 startingPage "123810" @default.
- W4382700800 abstract "A highly sensitive LC-MS/MS methods were developed and validated to quantify nine antiretrovirals (atazanavir [ATV], tenofovir [TFV], emtricitabine [FTC], darunavir [DRV], dolutegravir [DTG], efavirenz [EFV], lamivudine [3TC], raltegravir [RAL], and ritonavir [RTV]) in human cerebral spinal fluid (CSF). The approach remedies adsorption issues caused by polypropylene based sample collection tubes. 1% ammonium hydroxide in methanol was added in an amount equal to the volume of each quality control (QC) or patient sample. Protein precipitation was utilized with a CSF sample volume of 100 μL and a 100 μL of methanol:ACN and vortexed. Chromatographic separation was achieved with a 3 × 100 ACE® C18 column for ATV, DRV, DTG, EFV, RTV and RAL, and a 2 × 100 Polar RP column for TFV/FTC/3TC. Mobile phase was methanol:water:formic acid (70:30:0.1, v/v/v) for ATV, DRV, DTG, EFV and RTV (10 uL injection, flow rate: 1.00 mL/min), ACN:water:formic acid (35:65:0.1, v/v/v) for RAL (50 uL injection, flow rate: 1.00 mL/min), ACN:water:formic acid (2:98:0.1, v/v/v) for TFV, FTC and 3TC (50 uL injection, flow rate: 0.35 mL/min). Column temperature was 40° C across all assays. The mass spectrometer was operated in positive, multiple-reaction-monitoring (MRM) mode with electrospray ionization (ESI) for all analytes with the exception of EFV, which was operated in negative, MRM mode with ESI. The assay was linear over the calibration range of 1 to 250 ng/mL for all analytes. The addition of 1% ammonium hydroxide in sample tubes overcame up to 44% negative bias in QC samples and allowed the methods to meet full validation criteria." @default.
- W4382700800 created "2023-07-01" @default.
- W4382700800 creator A5000852039 @default.
- W4382700800 creator A5005928906 @default.
- W4382700800 creator A5017051528 @default.
- W4382700800 creator A5029625221 @default.
- W4382700800 creator A5031056065 @default.
- W4382700800 creator A5059217596 @default.
- W4382700800 creator A5064647938 @default.
- W4382700800 date "2023-07-01" @default.
- W4382700800 modified "2023-10-17" @default.
- W4382700800 title "Quantification of nine antiretroviral drugs in cerebrospinal fluid: An approach to overcome sample collection tube adsorption" @default.
- W4382700800 cites W1532754033 @default.
- W4382700800 cites W1969819920 @default.
- W4382700800 cites W1980280932 @default.
- W4382700800 cites W2045044146 @default.
- W4382700800 cites W2050226999 @default.
- W4382700800 cites W2077535491 @default.
- W4382700800 cites W2078467477 @default.
- W4382700800 cites W2085093423 @default.
- W4382700800 cites W2123530170 @default.
- W4382700800 cites W2124040992 @default.
- W4382700800 cites W2135732933 @default.
- W4382700800 cites W2156075763 @default.
- W4382700800 cites W2326437656 @default.
- W4382700800 cites W2762486448 @default.
- W4382700800 cites W3139360975 @default.
- W4382700800 cites W4316039467 @default.
- W4382700800 doi "https://doi.org/10.1016/j.jchromb.2023.123810" @default.
- W4382700800 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37429153" @default.
- W4382700800 hasPublicationYear "2023" @default.
- W4382700800 type Work @default.
- W4382700800 citedByCount "1" @default.
- W4382700800 crossrefType "journal-article" @default.
- W4382700800 hasAuthorship W4382700800A5000852039 @default.
- W4382700800 hasAuthorship W4382700800A5005928906 @default.
- W4382700800 hasAuthorship W4382700800A5017051528 @default.
- W4382700800 hasAuthorship W4382700800A5029625221 @default.
- W4382700800 hasAuthorship W4382700800A5031056065 @default.
- W4382700800 hasAuthorship W4382700800A5059217596 @default.
- W4382700800 hasAuthorship W4382700800A5064647938 @default.
- W4382700800 hasConcept C142462285 @default.
- W4382700800 hasConcept C159047783 @default.
- W4382700800 hasConcept C185592680 @default.
- W4382700800 hasConcept C2522874641 @default.
- W4382700800 hasConcept C2775986502 @default.
- W4382700800 hasConcept C2777182164 @default.
- W4382700800 hasConcept C2777379063 @default.
- W4382700800 hasConcept C2777869810 @default.
- W4382700800 hasConcept C2778414717 @default.
- W4382700800 hasConcept C2779182219 @default.
- W4382700800 hasConcept C2779298103 @default.
- W4382700800 hasConcept C2779502633 @default.
- W4382700800 hasConcept C2779778239 @default.
- W4382700800 hasConcept C2780404665 @default.
- W4382700800 hasConcept C2780593183 @default.
- W4382700800 hasConcept C2993143319 @default.
- W4382700800 hasConcept C3013748606 @default.
- W4382700800 hasConcept C43617362 @default.
- W4382700800 hasConcept C512399662 @default.
- W4382700800 hasConcept C71924100 @default.
- W4382700800 hasConcept C86803240 @default.
- W4382700800 hasConceptScore W4382700800C142462285 @default.
- W4382700800 hasConceptScore W4382700800C159047783 @default.
- W4382700800 hasConceptScore W4382700800C185592680 @default.
- W4382700800 hasConceptScore W4382700800C2522874641 @default.
- W4382700800 hasConceptScore W4382700800C2775986502 @default.
- W4382700800 hasConceptScore W4382700800C2777182164 @default.
- W4382700800 hasConceptScore W4382700800C2777379063 @default.
- W4382700800 hasConceptScore W4382700800C2777869810 @default.
- W4382700800 hasConceptScore W4382700800C2778414717 @default.
- W4382700800 hasConceptScore W4382700800C2779182219 @default.
- W4382700800 hasConceptScore W4382700800C2779298103 @default.
- W4382700800 hasConceptScore W4382700800C2779502633 @default.
- W4382700800 hasConceptScore W4382700800C2779778239 @default.
- W4382700800 hasConceptScore W4382700800C2780404665 @default.
- W4382700800 hasConceptScore W4382700800C2780593183 @default.
- W4382700800 hasConceptScore W4382700800C2993143319 @default.
- W4382700800 hasConceptScore W4382700800C3013748606 @default.
- W4382700800 hasConceptScore W4382700800C43617362 @default.
- W4382700800 hasConceptScore W4382700800C512399662 @default.
- W4382700800 hasConceptScore W4382700800C71924100 @default.
- W4382700800 hasConceptScore W4382700800C86803240 @default.
- W4382700800 hasFunder F4320332161 @default.
- W4382700800 hasFunder F4320337346 @default.
- W4382700800 hasFunder F4320337355 @default.
- W4382700800 hasLocation W43827008001 @default.
- W4382700800 hasLocation W43827008002 @default.
- W4382700800 hasOpenAccess W4382700800 @default.
- W4382700800 hasPrimaryLocation W43827008001 @default.
- W4382700800 hasRelatedWork W1550070317 @default.
- W4382700800 hasRelatedWork W1990854011 @default.
- W4382700800 hasRelatedWork W2001765285 @default.
- W4382700800 hasRelatedWork W2027613075 @default.
- W4382700800 hasRelatedWork W2144854003 @default.
- W4382700800 hasRelatedWork W2158292961 @default.
- W4382700800 hasRelatedWork W2341197564 @default.
- W4382700800 hasRelatedWork W2392737066 @default.