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- W4382893194 abstract "Abstract Background Alginate oligosaccharide (AOS) holds great potential as a novel feed supplement in farm animals. However, the effects of AOS on chicken health and the underlying mechanisms are not fully understood. This study aimed to optimize the enzymatic preparation of AOS by using bacterial alginate lyases expressed in yeast, investigate the effects of the prepared AOS on the growth performance and gut health of broiler chickens, and reveal the underlying mechanisms. Results Five alginate lyases from bacteria were cloned into Pichia pastoris GS115 and the alginate lyase PDE9 was expressed at relatively high yield, activity and stability in P. pastoris . Animal trials were carried out using 320 1-day-old male Arbor Acres broilers (four groups; 8 replicates/group × 10 chicks/replicate) receiving either a basal diet or the same diet supplemented with 100, 200 and 400 mg/kg PDE9-prepared AOS for 42 d. The results showed that dietary supplementation of 200 mg/kg AOS displayed the highest activity in promoting the birds’ ADG and ADFI ( P < 0.05). AOS ameliorated the intestinal morphology, absorption function and barrier function, as indicated by the enhanced ( P < 0.05) intestinal villus height, maltase activity, and the expression of PEPT , SGLT1 , ZNT1 , and occludin. AOS also increased serum insulin-like growth factor-1, ghrelin ( P < 0.05), and growth hormone ( P < 0.1). Moreover, the concentrations of acetate, isobutyrate, isovalerate, valerate, and total SCFAs in cecum of birds fed AOS were significantly higher than the control birds ( P < 0.05). Metagenomic analysis indicated that AOS modulated the chicken gut microbiota structure, function, and microbial interactions and promoted the growth of SCFAs-producing bacteria, for example, Dorea sp. 002160985; SCFAs, especially acetate, were found positively correlated with the chicken growth performance and growth-related hormone signals ( P < 0.05). We further verified that AOS can be utilized by Dorea sp. to grow and to produce acetate in vitro. Conclusions We demonstrated that the enzymatically produced AOS effectively promoted broiler chicken growth performance by modulating the chicken gut microbiota structure and function. For the first time, we established the connections among AOS, chicken gut microbiota/SCFAs, growth hormone signals and chicken growth performance. Graphical Abstract" @default.
- W4382893194 created "2023-07-03" @default.
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- W4382893194 date "2023-07-03" @default.
- W4382893194 modified "2023-10-09" @default.
- W4382893194 title "Enzymatically prepared alginate oligosaccharides improve broiler chicken growth performance by modulating the gut microbiota and growth hormone signals" @default.
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- W4382893194 cites W1977983497 @default.
- W4382893194 cites W1985381446 @default.
- W4382893194 cites W1986070075 @default.
- W4382893194 cites W1989887801 @default.
- W4382893194 cites W2009674410 @default.
- W4382893194 cites W2011312755 @default.
- W4382893194 cites W2025678536 @default.
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- W4382893194 cites W2058088516 @default.
- W4382893194 cites W2073295477 @default.
- W4382893194 cites W2082808164 @default.
- W4382893194 cites W2102303758 @default.
- W4382893194 cites W2107199606 @default.
- W4382893194 cites W2124171086 @default.
- W4382893194 cites W2125910575 @default.
- W4382893194 cites W2128258568 @default.
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- W4382893194 cites W2944717448 @default.
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- W4382893194 cites W2973529428 @default.
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- W4382893194 doi "https://doi.org/10.1186/s40104-023-00887-4" @default.
- W4382893194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37394467" @default.
- W4382893194 hasPublicationYear "2023" @default.
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