Matches in SemOpenAlex for { <https://semopenalex.org/work/W4382918423> ?p ?o ?g. }
- W4382918423 endingPage "1" @default.
- W4382918423 startingPage "1" @default.
- W4382918423 abstract "Rapid and accurate diagnosis of microbial keratitis (MK) could greatly improve patient outcomes. Here, we present the development of a rapid, accessible multicolour fluorescence imaging device (FluoroPi) and evaluate its performance in combination with fluorescent optical reporters (SmartProbes) to distinguish bacterial Gram status. Furthermore, we show feasibility by imaging samples obtained by corneal scrape and minimally invasive corneal impression membrane (CIM) from ex vivo porcine corneal MK models.FluoroPi was built using a Raspberry Pi single-board computer and camera, light-emitting-diodes (LEDs), and filters for white-light and fluorescent imaging, with excitation and detection of bacterial optical SmartProbes: Gram-negative, NBD-PMX (exmax 488 nm); Gram positive, Merocy-Van (exmax 590 nm). We evaluated FluoroPi with bacteria (Pseudomonas aeruginosa and Staphylococcus aureus) isolated from ex vivo porcine corneal models of MK by scrape (needle) and CIM with the SmartProbes.FluoroPi provides <1 µm resolution and was able to readily distinguish bacteria isolated from ex vivo models of MK from tissue debris when combined with SmartProbes, retrieved by both scrape and CIM. Single bacteria could be resolved within the field of view, with limits of detection demonstrated as 103 to 104 CFU/mL. Sample preparation prior to imaging was minimal (wash-free), and imaging and postprocessing with FluoroPi were straightforward, confirming ease of use.FluoroPi coupled with SmartProbes provides effective, low-cost bacterial imaging, delineating Gram-negative and Gram-positive bacteria directly sampled from a preclinical model of MK.This study provides a crucial stepping stone toward clinical translation of a rapid, minimally invasive diagnostic approach for MK." @default.
- W4382918423 created "2023-07-04" @default.
- W4382918423 creator A5026872522 @default.
- W4382918423 creator A5033537671 @default.
- W4382918423 creator A5039065537 @default.
- W4382918423 creator A5043581887 @default.
- W4382918423 creator A5045903049 @default.
- W4382918423 creator A5053576296 @default.
- W4382918423 creator A5054709225 @default.
- W4382918423 creator A5066465731 @default.
- W4382918423 creator A5070010097 @default.
- W4382918423 creator A5072122294 @default.
- W4382918423 creator A5079119400 @default.
- W4382918423 creator A5082188821 @default.
- W4382918423 date "2023-07-03" @default.
- W4382918423 modified "2023-09-27" @default.
- W4382918423 title "FluoroPi Device With SmartProbes: A Frugal Point-of-Care System for Fluorescent Detection of Bacteria From a Pre-Clinical Model of Microbial Keratitis" @default.
- W4382918423 cites W1622925613 @default.
- W4382918423 cites W1960966027 @default.
- W4382918423 cites W1968857708 @default.
- W4382918423 cites W2037113806 @default.
- W4382918423 cites W2052780047 @default.
- W4382918423 cites W2161433272 @default.
- W4382918423 cites W2291646715 @default.
- W4382918423 cites W2345479071 @default.
- W4382918423 cites W2794482948 @default.
- W4382918423 cites W2887460592 @default.
- W4382918423 cites W2898437989 @default.
- W4382918423 cites W2901675861 @default.
- W4382918423 cites W2905636906 @default.
- W4382918423 cites W2949327116 @default.
- W4382918423 cites W2952739892 @default.
- W4382918423 cites W2969316895 @default.
- W4382918423 cites W2971865686 @default.
- W4382918423 cites W2975523910 @default.
- W4382918423 cites W2987740105 @default.
- W4382918423 cites W2990509551 @default.
- W4382918423 cites W3006888474 @default.
- W4382918423 cites W3008960266 @default.
- W4382918423 cites W3012392814 @default.
- W4382918423 cites W3026918274 @default.
- W4382918423 cites W3036081527 @default.
- W4382918423 cites W3042011755 @default.
- W4382918423 cites W3048134315 @default.
- W4382918423 cites W3099981592 @default.
- W4382918423 cites W3111487495 @default.
- W4382918423 cites W3130472894 @default.
- W4382918423 cites W3134769625 @default.
- W4382918423 cites W3138437179 @default.
- W4382918423 cites W3156715596 @default.
- W4382918423 cites W3169580339 @default.
- W4382918423 cites W3215256801 @default.
- W4382918423 cites W3215329953 @default.
- W4382918423 cites W4200409424 @default.
- W4382918423 cites W4220781615 @default.
- W4382918423 cites W4226207691 @default.
- W4382918423 cites W4236302859 @default.
- W4382918423 cites W4280499911 @default.
- W4382918423 cites W4285501386 @default.
- W4382918423 cites W4289712099 @default.
- W4382918423 doi "https://doi.org/10.1167/tvst.12.7.1" @default.
- W4382918423 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37395707" @default.
- W4382918423 hasPublicationYear "2023" @default.
- W4382918423 type Work @default.
- W4382918423 citedByCount "0" @default.
- W4382918423 crossrefType "journal-article" @default.
- W4382918423 hasAuthorship W4382918423A5026872522 @default.
- W4382918423 hasAuthorship W4382918423A5033537671 @default.
- W4382918423 hasAuthorship W4382918423A5039065537 @default.
- W4382918423 hasAuthorship W4382918423A5043581887 @default.
- W4382918423 hasAuthorship W4382918423A5045903049 @default.
- W4382918423 hasAuthorship W4382918423A5053576296 @default.
- W4382918423 hasAuthorship W4382918423A5054709225 @default.
- W4382918423 hasAuthorship W4382918423A5066465731 @default.
- W4382918423 hasAuthorship W4382918423A5070010097 @default.
- W4382918423 hasAuthorship W4382918423A5072122294 @default.
- W4382918423 hasAuthorship W4382918423A5079119400 @default.
- W4382918423 hasAuthorship W4382918423A5082188821 @default.
- W4382918423 hasBestOaLocation W43829184231 @default.
- W4382918423 hasConcept C118487528 @default.
- W4382918423 hasConcept C120665830 @default.
- W4382918423 hasConcept C121332964 @default.
- W4382918423 hasConcept C136229726 @default.
- W4382918423 hasConcept C150903083 @default.
- W4382918423 hasConcept C207001950 @default.
- W4382918423 hasConcept C26291073 @default.
- W4382918423 hasConcept C2778560704 @default.
- W4382918423 hasConcept C29331040 @default.
- W4382918423 hasConcept C523546767 @default.
- W4382918423 hasConcept C54355233 @default.
- W4382918423 hasConcept C57477423 @default.
- W4382918423 hasConcept C71924100 @default.
- W4382918423 hasConcept C86803240 @default.
- W4382918423 hasConcept C89423630 @default.
- W4382918423 hasConcept C91881484 @default.
- W4382918423 hasConceptScore W4382918423C118487528 @default.
- W4382918423 hasConceptScore W4382918423C120665830 @default.
- W4382918423 hasConceptScore W4382918423C121332964 @default.