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- W4382932151 abstract "Wood-decaying fungi are the major decomposers of plant litter. Heavy sequencing efforts on genomes of wood-decaying fungi have recently been made due to the interest in their lignocellulolytic enzymes; however, most parts of their proteomes remain uncharted. We hypothesized that wood-decaying fungi would possess promiscuous enzymes for detoxifying antifungal phytochemicals remaining in the dead plant bodies, which can be useful biocatalysts. We designed a computational mass spectrometry-based untargeted metabolomics pipeline for the phenotyping of biotransformation and applied it to 264 fungal cultures supplemented with antifungal plant phenolics. The analysis identified the occurrence of diverse reactivities by the tested fungal species. Among those, we focused on O-xylosylation of multiple phenolics by one of the species tested, Lentinus brumalis. By integrating the metabolic phenotyping results with publicly available genome sequences and transcriptome analysis, a UDP-glycosyltransferase designated UGT66A1 was identified and validated as an enzyme catalyzing O-xylosylation with broad substrate specificity. We anticipate that our analytical workflow will accelerate the further characterization of fungal enzymes as promising biocatalysts." @default.
- W4382932151 created "2023-07-04" @default.
- W4382932151 creator A5008687469 @default.
- W4382932151 creator A5008771490 @default.
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- W4382932151 creator A5048838973 @default.
- W4382932151 creator A5067045439 @default.
- W4382932151 creator A5067929529 @default.
- W4382932151 date "2023-07-03" @default.
- W4382932151 modified "2023-09-23" @default.
- W4382932151 title "Qualitative metabolomics-based characterization of a phenolic UDP-xylosyltransferase with a broad substrate spectrum from <i>Lentinus brumalis</i>" @default.
- W4382932151 cites W1496768249 @default.
- W4382932151 cites W1730146913 @default.
- W4382932151 cites W1916210473 @default.
- W4382932151 cites W1966156878 @default.
- W4382932151 cites W1987916789 @default.
- W4382932151 cites W1995462232 @default.
- W4382932151 cites W1995727180 @default.
- W4382932151 cites W2008466703 @default.
- W4382932151 cites W2010574143 @default.
- W4382932151 cites W2039531605 @default.
- W4382932151 cites W2041504287 @default.
- W4382932151 cites W2047015012 @default.
- W4382932151 cites W2056460576 @default.
- W4382932151 cites W2057194258 @default.
- W4382932151 cites W2067944225 @default.
- W4382932151 cites W2077646314 @default.
- W4382932151 cites W2077958668 @default.
- W4382932151 cites W2094199952 @default.
- W4382932151 cites W2114104545 @default.
- W4382932151 cites W2115888213 @default.
- W4382932151 cites W2121004444 @default.
- W4382932151 cites W2124019798 @default.
- W4382932151 cites W2127774996 @default.
- W4382932151 cites W2130479394 @default.
- W4382932151 cites W2134526812 @default.
- W4382932151 cites W2137015675 @default.
- W4382932151 cites W2143650174 @default.
- W4382932151 cites W2144803530 @default.
- W4382932151 cites W2152207030 @default.
- W4382932151 cites W2164542014 @default.
- W4382932151 cites W2201488053 @default.
- W4382932151 cites W2314129918 @default.
- W4382932151 cites W2333192041 @default.
- W4382932151 cites W2473631582 @default.
- W4382932151 cites W2504691963 @default.
- W4382932151 cites W2519072018 @default.
- W4382932151 cites W2584376366 @default.
- W4382932151 cites W2584396073 @default.
- W4382932151 cites W2587186776 @default.
- W4382932151 cites W2588029285 @default.
- W4382932151 cites W2588824507 @default.
- W4382932151 cites W2598566546 @default.
- W4382932151 cites W2750791471 @default.
- W4382932151 cites W2761631498 @default.
- W4382932151 cites W2769958019 @default.
- W4382932151 cites W2783237166 @default.
- W4382932151 cites W2788936996 @default.
- W4382932151 cites W2803399729 @default.
- W4382932151 cites W2804186471 @default.
- W4382932151 cites W2805541372 @default.
- W4382932151 cites W2883449263 @default.
- W4382932151 cites W2883818909 @default.
- W4382932151 cites W2914241247 @default.
- W4382932151 cites W2916918683 @default.
- W4382932151 cites W2921669367 @default.
- W4382932151 cites W2944480868 @default.
- W4382932151 cites W2948160364 @default.
- W4382932151 cites W2948341724 @default.
- W4382932151 cites W2950468843 @default.
- W4382932151 cites W2956920153 @default.
- W4382932151 cites W2964849163 @default.
- W4382932151 cites W2965574859 @default.
- W4382932151 cites W2973890758 @default.
- W4382932151 cites W2977545601 @default.
- W4382932151 cites W2979692748 @default.
- W4382932151 cites W2996407698 @default.
- W4382932151 cites W3034548692 @default.
- W4382932151 cites W3080789666 @default.
- W4382932151 cites W3092358737 @default.
- W4382932151 cites W3159045280 @default.
- W4382932151 cites W3177261807 @default.
- W4382932151 cites W3177828909 @default.
- W4382932151 cites W3200557693 @default.
- W4382932151 cites W3211795435 @default.
- W4382932151 cites W4206117801 @default.
- W4382932151 cites W4206250909 @default.
- W4382932151 cites W4223551334 @default.
- W4382932151 cites W4239792147 @default.
- W4382932151 cites W4244815793 @default.
- W4382932151 cites W4251751280 @default.
- W4382932151 cites W4280507115 @default.
- W4382932151 cites W4281790889 @default.
- W4382932151 cites W4288096104 @default.
- W4382932151 cites W4290547242 @default.