FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4383215539> ?p ?o ?g. }

• W4383215539 endingPage "046001" @default.
• W4383215539 startingPage "046001" @default.
• W4383215539 abstract "The use of volatile biomarkers in exhaled breath as predictors to individual drug response would advance the field of personalised medicine by providing direct information on enzyme activity. This would result in enormous benefits, both for patients and for the healthcare sector. Non-invasive breath tests would also gain a high acceptance by patients. Towards this goal, differences in metabolism resulting from extensive polymorphisms in a major group of drug-metabolizing enzymes, the cytochrome P450 (CYP) family, need to be determined and quantified. CYP2C9 is responsible for metabolising many crucial drugs (e.g., diclofenac) and food ingredients (e.g., limonene). In this paper, we provide a proof-of-concept study that illustrates thein vitrobioconversion of diclofenac in recombinant HEK293T cells overexpressing CYP2C9 to 4'-hydroxydiclofenac. Thisin vitroapproach is a necessary and important first step in the development of breath tests to determine and monitor metabolic processes in the human body. By focusing on the metabolic conversion of diclofenac, we have been able to establish a workflow using a cell-based system for CYP2C9 activity. Furthermore, we illustrate how the bioconversion of diclofenac is limited in the presence of limonene, which is another CYP2C9 metabolising substrate. We show that increasing limonene levels continuously reduce the production of 4'-hydroxydiclofenac. Michaelis-Menten kinetics were performed for the diclofenac 4'-hydroxylation with and without limonene, giving a kinetic constant of the reaction,KM, of 103µM and 94.1µM, respectively, and a maximum reaction rate,Vmax, of 46.8 pmol min-1106cells-1and 56.0 pmol min-1106cells-1with and without the inhibitor, respectively, suggesting a non-competitive or mixed inhibition type. The half-maximal inhibitory concentration value (IC50) for the inhibition of the formation of 4'-hydroxydiclofenace by limonene is determined to be 1413µM." @default.
• W4383215539 created "2023-07-06" @default.
• W4383215539 creator A5000379524 @default.
• W4383215539 creator A5008315598 @default.
• W4383215539 creator A5013635280 @default.
• W4383215539 creator A5028405436 @default.
• W4383215539 creator A5031445293 @default.
• W4383215539 creator A5034034027 @default.
• W4383215539 creator A5058960049 @default.
• W4383215539 creator A5069465132 @default.
• W4383215539 creator A5074539161 @default.
• W4383215539 creator A5092403337 @default.
• W4383215539 date "2023-07-19" @default.
• W4383215539 modified "2023-10-18" @default.
• W4383215539 title "Establishing a cell-based screening workflow for determining the efficiency of CYP2C9 metabolism: moving towards the use of breath volatiles in personalised medicine" @default.
• W4383215539 cites W1967089707 @default.
• W4383215539 cites W1971721185 @default.
• W4383215539 cites W1977931991 @default.
• W4383215539 cites W201125075 @default.
• W4383215539 cites W2030018298 @default.
• W4383215539 cites W2037116391 @default.
• W4383215539 cites W2047471874 @default.
• W4383215539 cites W2049126525 @default.
• W4383215539 cites W2092160410 @default.
• W4383215539 cites W2126983356 @default.
• W4383215539 cites W2141115118 @default.
• W4383215539 cites W2165455460 @default.
• W4383215539 cites W2269046786 @default.
• W4383215539 cites W2345433402 @default.
• W4383215539 cites W2525611773 @default.
• W4383215539 cites W2538201653 @default.
• W4383215539 cites W2609070199 @default.
• W4383215539 cites W2763920048 @default.
• W4383215539 cites W2893926758 @default.
• W4383215539 cites W355643970 @default.
• W4383215539 doi "https://doi.org/10.1088/1752-7163/ace46f" @default.
• W4383215539 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37406623" @default.
• W4383215539 hasPublicationYear "2023" @default.
• W4383215539 type Work @default.
• W4383215539 citedByCount "0" @default.
• W4383215539 crossrefType "journal-article" @default.
• W4383215539 hasAuthorship W4383215539A5000379524 @default.
• W4383215539 hasAuthorship W4383215539A5008315598 @default.
• W4383215539 hasAuthorship W4383215539A5013635280 @default.
• W4383215539 hasAuthorship W4383215539A5028405436 @default.
• W4383215539 hasAuthorship W4383215539A5031445293 @default.
• W4383215539 hasAuthorship W4383215539A5034034027 @default.
• W4383215539 hasAuthorship W4383215539A5058960049 @default.
• W4383215539 hasAuthorship W4383215539A5069465132 @default.
• W4383215539 hasAuthorship W4383215539A5074539161 @default.
• W4383215539 hasAuthorship W4383215539A5092403337 @default.
• W4383215539 hasBestOaLocation W43832155391 @default.
• W4383215539 hasConcept C100544194 @default.
• W4383215539 hasConcept C126322002 @default.
• W4383215539 hasConcept C181199279 @default.
• W4383215539 hasConcept C185592680 @default.
• W4383215539 hasConcept C2776054734 @default.
• W4383215539 hasConcept C2779306644 @default.
• W4383215539 hasConcept C2779826273 @default.
• W4383215539 hasConcept C2780074830 @default.
• W4383215539 hasConcept C27881333 @default.
• W4383215539 hasConcept C3946865 @default.
• W4383215539 hasConcept C526171541 @default.
• W4383215539 hasConcept C55493867 @default.
• W4383215539 hasConcept C71924100 @default.
• W4383215539 hasConcept C98274493 @default.
• W4383215539 hasConceptScore W4383215539C100544194 @default.
• W4383215539 hasConceptScore W4383215539C126322002 @default.
• W4383215539 hasConceptScore W4383215539C181199279 @default.
• W4383215539 hasConceptScore W4383215539C185592680 @default.
• W4383215539 hasConceptScore W4383215539C2776054734 @default.
• W4383215539 hasConceptScore W4383215539C2779306644 @default.
• W4383215539 hasConceptScore W4383215539C2779826273 @default.
• W4383215539 hasConceptScore W4383215539C2780074830 @default.
• W4383215539 hasConceptScore W4383215539C27881333 @default.
• W4383215539 hasConceptScore W4383215539C3946865 @default.
• W4383215539 hasConceptScore W4383215539C526171541 @default.
• W4383215539 hasConceptScore W4383215539C55493867 @default.
• W4383215539 hasConceptScore W4383215539C71924100 @default.
• W4383215539 hasConceptScore W4383215539C98274493 @default.
• W4383215539 hasFunder F4320321181 @default.
• W4383215539 hasIssue "4" @default.
• W4383215539 hasLocation W43832155391 @default.
• W4383215539 hasLocation W43832155392 @default.
• W4383215539 hasOpenAccess W4383215539 @default.
• W4383215539 hasPrimaryLocation W43832155391 @default.
• W4383215539 hasRelatedWork W2006233586 @default.
• W4383215539 hasRelatedWork W2048796575 @default.
• W4383215539 hasRelatedWork W2106301847 @default.
• W4383215539 hasRelatedWork W2149319043 @default.
• W4383215539 hasRelatedWork W2276236254 @default.
• W4383215539 hasRelatedWork W2323140218 @default.
• W4383215539 hasRelatedWork W2391377592 @default.
• W4383215539 hasRelatedWork W2416280168 @default.
• W4383215539 hasRelatedWork W2612119074 @default.
• W4383215539 hasRelatedWork W2995441305 @default.
• W4383215539 hasVolume "17" @default.
• W4383215539 isParatext "false" @default.

• next