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- W4383215616 abstract "Abstract Mutations in DNA damage response (DDR) factors are associated with human infertility, which affects up to 15% of the population. It remains unclear if the role of DDR is solely in meiosis. One pathway implicated in human fertility is DNA translesion synthesis (TLS), which allows replication impediments to be bypassed. We find that TLS is essential for pre-meiotic germ cell development in the embryo. Loss of the central TLS component, REV1, significantly inhibits the induction of human PGC-like cells (hPGCLCs). This is recapitulated in mice, where deficiencies in TLS initiation ( Rev1 -/- or Pcna K164R/K164R ) or extension ( Rev7 -/- ) result in a >150-fold reduction in the number of primordial germ cells (PGCs) and complete sterility. In contrast, the absence of TLS does not impact the growth, function, or homeostasis of somatic tissues. Surprisingly, we find a complete failure in both activation of the germ cell transcriptional program and in DNA demethylation, a critical step in germline epigenetic reprogramming. Our findings show that for normal fertility, DNA repair is required not only for meiotic recombination but for progression through the earliest stages of germ cell development in mammals." @default.
- W4383215616 created "2023-07-06" @default.
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- W4383215616 date "2023-07-05" @default.
- W4383215616 modified "2023-10-01" @default.
- W4383215616 title "Primordial germ cell DNA demethylation and development require DNA translesion synthesis" @default.
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- W4383215616 doi "https://doi.org/10.1101/2023.07.05.547775" @default.
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