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• W4383313462 abstract "Abstract Rapid, on-site, airborne virus detection is a requirement for timely action against the spread of air-transmissible infectious diseases. This applies both to future threats and to common viral diseases, such as influenza and COVID-19, which hit vulnerable populations yearly with severe consequences. The ultra-low concentrations of virus in the air make airborne virus detection difficult, yet readily infect individuals when breathed. Here, we propose a fieldable process that includes an enrichment step to concentrate collected genetic material in a small volume. The enrichment approach uses capillary electrophoresis and an RT-qPCR-compatible buffer, which allow enrichment of the RNA by about 5-fold within only 10 minutes of operation. Our detection process consists of air sampling through electrostatic precipitation, RNA extraction via heating, RNA enrichment, and RT-qPCR for detection. We optimized each step of the process and estimated a detection sensitivity of 3106 ± 2457 genome copies (gc) per m 3 of air. We then performed an integration experiment and confirmed a sensitivity of 5654 gc/m 3 with a detection rate of 100% and a sensitivity of 4221 gc/m 3 with a detection rate of 78.6%. When using fast RT-qPCR, the latency of the whole process is down to 61 minutes. Given that our sensitivity falls in the low range of influenza and SARS-CoV-2 concentrations reported in indoor spaces, our study shows that, with enrichment, airborne pathogen detection can be made sufficiently sensitive for practical use." @default.
• W4383313462 created "2023-07-07" @default.
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• W4383313462 date "2023-07-06" @default.
• W4383313462 modified "2023-09-30" @default.
• W4383313462 title "Characterization of a fieldable process for airborne virus detection" @default.
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• W4383313462 doi "https://doi.org/10.1101/2023.07.03.23292170" @default.
• W4383313462 hasPublicationYear "2023" @default.
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