Matches in SemOpenAlex for { <https://semopenalex.org/work/W4383314757> ?p ?o ?g. }
- W4383314757 endingPage "25287" @default.
- W4383314757 startingPage "25279" @default.
- W4383314757 abstract "Insulin undergoes agglomeration with (subtle) changes in its biochemical environment, including acidity, application of heat, ionic imbalance, and exposure to hydrophobic surfaces. The therapeutic impact of such unwarranted insulin agglomeration is unclear and needs further evaluation. A systematic investigation was conducted on recombinant human insulin-with or without labeling with fluorescein isothiocyanate-while preparing insulin suspensions (0.125, 0.25, and 0.5 mg/mL) at pH 3. The suspensions were incubated (37 °C) and analyzed at different time points (t = 2, 4, 24, 48, and 72 h). Transmission electron microscopy and nanoparticle tracking analysis identified colloidally stable (zeta potential 15 ± 5 mV) spherical agglomerates of unlabeled insulin (100-500 nm). Circular dichroism established the preservation of insulin's secondary structure rich in α-helices despite exposure to an acidic environment (pH 3) for 72 h. Furthermore, fluorescence lifetime imaging microscopy illustrated an acidic core inside these spherical agglomerates, while the acidity gradually lessened toward the periphery. Some of these smaller agglomerates fused to form larger chunks with discrete zones of acidity. The data indicated a primary nucleation-driven mechanism of acid-induced insulin agglomeration under physiologically relevant conditions." @default.
- W4383314757 created "2023-07-07" @default.
- W4383314757 creator A5008657886 @default.
- W4383314757 creator A5033265502 @default.
- W4383314757 creator A5043120743 @default.
- W4383314757 creator A5083086715 @default.
- W4383314757 creator A5092410334 @default.
- W4383314757 date "2023-07-06" @default.
- W4383314757 modified "2023-10-18" @default.
- W4383314757 title "An Investigation into the Acidity-Induced Insulin Agglomeration: Implications for Drug Delivery and Translation" @default.
- W4383314757 cites W1245801083 @default.
- W4383314757 cites W1588979652 @default.
- W4383314757 cites W1954429618 @default.
- W4383314757 cites W1964161207 @default.
- W4383314757 cites W1971052273 @default.
- W4383314757 cites W1972011321 @default.
- W4383314757 cites W1985563927 @default.
- W4383314757 cites W2005212584 @default.
- W4383314757 cites W2005228679 @default.
- W4383314757 cites W2023215494 @default.
- W4383314757 cites W2043282395 @default.
- W4383314757 cites W2049304205 @default.
- W4383314757 cites W2059804089 @default.
- W4383314757 cites W2076190884 @default.
- W4383314757 cites W2081372700 @default.
- W4383314757 cites W2084224582 @default.
- W4383314757 cites W2134922062 @default.
- W4383314757 cites W2148892150 @default.
- W4383314757 cites W2155323783 @default.
- W4383314757 cites W2157151585 @default.
- W4383314757 cites W2304355334 @default.
- W4383314757 cites W2309356304 @default.
- W4383314757 cites W2320748066 @default.
- W4383314757 cites W2322525830 @default.
- W4383314757 cites W2771230435 @default.
- W4383314757 cites W2775665861 @default.
- W4383314757 cites W2782767874 @default.
- W4383314757 cites W2802118516 @default.
- W4383314757 cites W2805255143 @default.
- W4383314757 cites W2885167831 @default.
- W4383314757 cites W2887352774 @default.
- W4383314757 cites W2972869264 @default.
- W4383314757 cites W2992229611 @default.
- W4383314757 cites W3013973547 @default.
- W4383314757 cites W3038210287 @default.
- W4383314757 cites W3067029739 @default.
- W4383314757 cites W3081655479 @default.
- W4383314757 cites W3084346878 @default.
- W4383314757 cites W3126631165 @default.
- W4383314757 cites W3162638752 @default.
- W4383314757 cites W3172198976 @default.
- W4383314757 cites W3199685641 @default.
- W4383314757 cites W4200000793 @default.
- W4383314757 cites W4205846747 @default.
- W4383314757 cites W4205863490 @default.
- W4383314757 cites W4206916963 @default.
- W4383314757 cites W4225413983 @default.
- W4383314757 cites W4280502047 @default.
- W4383314757 cites W4283210749 @default.
- W4383314757 cites W4304756380 @default.
- W4383314757 doi "https://doi.org/10.1021/acsomega.3c02482" @default.
- W4383314757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37483254" @default.
- W4383314757 hasPublicationYear "2023" @default.
- W4383314757 type Work @default.
- W4383314757 citedByCount "0" @default.
- W4383314757 crossrefType "journal-article" @default.
- W4383314757 hasAuthorship W4383314757A5008657886 @default.
- W4383314757 hasAuthorship W4383314757A5033265502 @default.
- W4383314757 hasAuthorship W4383314757A5043120743 @default.
- W4383314757 hasAuthorship W4383314757A5083086715 @default.
- W4383314757 hasAuthorship W4383314757A5092410334 @default.
- W4383314757 hasBestOaLocation W43833147571 @default.
- W4383314757 hasConcept C121332964 @default.
- W4383314757 hasConcept C12554922 @default.
- W4383314757 hasConcept C126322002 @default.
- W4383314757 hasConcept C127413603 @default.
- W4383314757 hasConcept C129955480 @default.
- W4383314757 hasConcept C131850264 @default.
- W4383314757 hasConcept C133571119 @default.
- W4383314757 hasConcept C145148216 @default.
- W4383314757 hasConcept C155672457 @default.
- W4383314757 hasConcept C171250308 @default.
- W4383314757 hasConcept C178790620 @default.
- W4383314757 hasConcept C185592680 @default.
- W4383314757 hasConcept C192562407 @default.
- W4383314757 hasConcept C2182769 @default.
- W4383314757 hasConcept C2777019933 @default.
- W4383314757 hasConcept C2779306644 @default.
- W4383314757 hasConcept C42360764 @default.
- W4383314757 hasConcept C55493867 @default.
- W4383314757 hasConcept C61048295 @default.
- W4383314757 hasConcept C62520636 @default.
- W4383314757 hasConcept C71924100 @default.
- W4383314757 hasConcept C86181022 @default.
- W4383314757 hasConcept C86803240 @default.
- W4383314757 hasConcept C91881484 @default.
- W4383314757 hasConceptScore W4383314757C121332964 @default.
- W4383314757 hasConceptScore W4383314757C12554922 @default.