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- W4383315072 abstract "Abstract Flavivirids are small, enveloped, positive-sense RNA viruses from the Flaviviridae family with genomes between ∼9-13kb. Metatranscriptomic analyses of metazoan organisms have revealed a diversity of flavivirus-like or flavivirid viral sequences in fish and marine invertebrate groups. To date, however, no flavivirus-like or flavivirid has been identified in amphibians. To remedy this, we investigated the virome of the European common frog ( Rana temporaria ) in the United Kingdom, utilising high-throughput sequencing at six catch locations. De novo assembly revealed a coding-complete virus contig of a novel flavivirid ∼11.2kb in length. The virus encodes a single open reading frame of 3456 amino acids and 5’ and 3’ untranslated regions (UTRs) of 227 and 666nt, respectively. We named this virus Rana tamanavirus (RaTV), as BLASTp analysis of the polyprotein showed the closest relationships to Tamana bat virus (TABV) and Cyclopterus lumpus virus from Pteronotus parnellii and Cyclopterus lumpus , respectively. Phylogenetic analysis of the RaTV polyprotein compared to Flavivirus and Flavivirus-like members indicated that RaTV was sufficiently divergent and basal to the vertebrate Tamanavirus clade. In addition to the Mitcham strain, partial but divergent RaTV, 95.64-97.39% pairwise nucleotide identity, were also obtained from the Poole and Deal samples, indicating that RaTV is widespread in UK frog samples. Bioinformatic analyses of putative secondary structures in the 3′-UTR of RaTV indicated a potential exoribonuclease-resistant RNA (xrRNA) structure identified in flaviviruses and TABV. To examine this biochemically, we conducted an in vitro XRN1 digestion assay showing that RaTV likely forms a divergent but functionally homologous XRN1-resistant xrRNA." @default.
- W4383315072 created "2023-07-07" @default.
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- W4383315072 date "2023-07-06" @default.
- W4383315072 modified "2023-10-06" @default.
- W4383315072 title "A novel tamanavirus (<i>Flaviviridae</i>) of the European common frog (<i>Rana temporaria</i>) encodes a divergent class 1b XRN1-resistant RNA element" @default.
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- W4383315072 doi "https://doi.org/10.1101/2023.07.06.547906" @default.
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