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• W4383345487 endingPage "961" @default.
• W4383345487 startingPage "961" @default.
• W4383345487 abstract "The apicomplexan protozoan parasite Cryptosporidium parvum is responsible for cryptosporidiosis, which is a zoonotic intestinal illness that affects newborn cattle, wild animals, and people all over the world. Mammalian monocytes are bone marrow-derived myeloid leukocytes with important defense effector functions in early host innate immunity due to their ATP purinergic-, CD14- and CD16-receptors, adhesion, migration and phagocytosis capacities, inflammatory, and anti-parasitic properties. The formation of monocyte extracellular traps (METs) has recently been reported as an additional effector mechanism against apicomplexan parasites. Nonetheless, nothing is known in the literature on METs extrusion neither towards C. parvum-oocysts nor sporozoites. Herein, ATP purinergic receptor P2X1, glycolysis, Notch signaling, and lactate monocarboxylate transporters (MCT) were investigated in C. parvum-exposed bovine monocytes under intestinal physioxia (5% O2) and hyperoxia (21% O2; most commonly used hyperoxic laboratory conditions). C. parvum-triggered suicidal METs were confirmed by complete rupture of exposed monocytes, co-localization of extracellular DNA with myeloperoxidase (MPO) and histones (H1-H4) via immunofluorescence- and confocal microscopy analyses. C. parvum-induced suicidal METs resulted not only in oocyst entrapment but also in hindered sporozoite mobility from oocysts according to scanning electron microscopy (SEM) analyses. Early parasite-induced bovine monocyte activation, accompanied by membrane protrusions toward C. parvum-oocysts/sporozoites, was unveiled using live cell 3D-holotomographic microscopy analysis. The administration of NF449, an inhibitor of the ATP purinergic receptor P2X1, to monocytes subjected to varying oxygen concentrations did not yield a noteworthy decrease in C. parvum-induced METosis. This suggests that the cell death process is not dependent on P2X1. Additionally, blockage of glycolysis in monocyte through 2-deoxy glucose (2-DG) inhibition reduced C. parvum-induced METosis but not significantly. According to monocyte energetic state measurements, C. parvum-exposed cells neither increased extracellular acidification rates (ECAR) nor oxygen consumption rates (OCR). Lactate monocarboxylate transporters (MCT) inhibitor (i.e., AR-C 141990) treatments significantly diminished C. parvum-mediated METs extrusion under physioxic (5% O2) condition. Similarly, treatment with either DAPT or compound E, two selective Notch inhibitors, exhibited no significant suppressive effects on bovine MET production. Overall, for the first time, we demonstrate C. parvum-mediated METosis as P2X1-independent but as an MCT-dependent defense mechanism under intestinal physioxia (5% CO2) conditions. METs findings suggest anti-cryptosporidial effects through parasite entrapment and inhibition of sporozoite excystation." @default.
• W4383345487 created "2023-07-07" @default.
• W4383345487 creator A5005407638 @default.
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• W4383345487 creator A5045679978 @default.
• W4383345487 creator A5068616844 @default.
• W4383345487 date "2023-07-05" @default.
• W4383345487 modified "2023-09-25" @default.
• W4383345487 title "MCT-Dependent Cryptosporidium parvum-Induced Bovine Monocyte Extracellular Traps (METs) under Physioxia" @default.
• W4383345487 cites W1531140346 @default.
• W4383345487 cites W1537725978 @default.
• W4383345487 cites W1557707473 @default.
• W4383345487 cites W1558203474 @default.
• W4383345487 cites W1575083490 @default.
• W4383345487 cites W1658920532 @default.
• W4383345487 cites W1794491409 @default.
• W4383345487 cites W1900401950 @default.
• W4383345487 cites W1965859404 @default.
• W4383345487 cites W1965987169 @default.
• W4383345487 cites W1971514874 @default.
• W4383345487 cites W1977719584 @default.
• W4383345487 cites W1997089052 @default.
• W4383345487 cites W1999186203 @default.
• W4383345487 cites W2009044009 @default.
• W4383345487 cites W2009809214 @default.
• W4383345487 cites W2010146173 @default.
• W4383345487 cites W2019210852 @default.
• W4383345487 cites W2027786827 @default.
• W4383345487 cites W2031461397 @default.
• W4383345487 cites W2031777430 @default.
• W4383345487 cites W2041249305 @default.
• W4383345487 cites W2055438942 @default.
• W4383345487 cites W2059403792 @default.
• W4383345487 cites W2060669952 @default.
• W4383345487 cites W2073036148 @default.
• W4383345487 cites W2074420568 @default.
• W4383345487 cites W2075535173 @default.
• W4383345487 cites W2077305243 @default.
• W4383345487 cites W2077743280 @default.
• W4383345487 cites W2078660855 @default.
• W4383345487 cites W2079434849 @default.
• W4383345487 cites W2080103109 @default.
• W4383345487 cites W2082126252 @default.
• W4383345487 cites W2085099489 @default.
• W4383345487 cites W2086281197 @default.
• W4383345487 cites W2090276247 @default.
• W4383345487 cites W2091959285 @default.
• W4383345487 cites W2094317942 @default.
• W4383345487 cites W2094794898 @default.
• W4383345487 cites W2114053580 @default.
• W4383345487 cites W2114749317 @default.
• W4383345487 cites W2116797415 @default.
• W4383345487 cites W2127385800 @default.
• W4383345487 cites W2127690773 @default.
• W4383345487 cites W2128337342 @default.
• W4383345487 cites W2130015846 @default.
• W4383345487 cites W2130253814 @default.
• W4383345487 cites W2140705832 @default.
• W4383345487 cites W2150864606 @default.
• W4383345487 cites W2154272953 @default.
• W4383345487 cites W2155954662 @default.
• W4383345487 cites W2163194429 @default.
• W4383345487 cites W2167692009 @default.
• W4383345487 cites W2170533080 @default.
• W4383345487 cites W2217024838 @default.
• W4383345487 cites W2217623857 @default.
• W4383345487 cites W2258966438 @default.
• W4383345487 cites W2291471126 @default.
• W4383345487 cites W2313372982 @default.
• W4383345487 cites W2396160531 @default.
• W4383345487 cites W2408984497 @default.
• W4383345487 cites W2463685051 @default.
• W4383345487 cites W2485291278 @default.
• W4383345487 cites W2522866345 @default.
• W4383345487 cites W2611615951 @default.
• W4383345487 cites W2738391531 @default.
• W4383345487 cites W2744448906 @default.
• W4383345487 cites W2748091479 @default.
• W4383345487 cites W2753584011 @default.
• W4383345487 cites W2777384123 @default.
• W4383345487 cites W2785130291 @default.
• W4383345487 cites W2805625964 @default.
• W4383345487 cites W2885976665 @default.
• W4383345487 cites W2891747209 @default.
• W4383345487 cites W2902849991 @default.
• W4383345487 cites W2908670804 @default.
• W4383345487 cites W2916495078 @default.
• W4383345487 cites W2939109981 @default.
• W4383345487 cites W2943391614 @default.
• W4383345487 cites W2943415163 @default.
• W4383345487 cites W2949231042 @default.
• W4383345487 cites W2955084980 @default.
• W4383345487 cites W2981587575 @default.
• W4383345487 cites W2988086740 @default.
• W4383345487 cites W2990498627 @default.
• W4383345487 cites W3042082254 @default.

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