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- W4383346740 abstract "Cells control actin assembly by regulating reactions at actin filament barbed ends. Formins accelerate elongation, capping protein (CP) arrests growth and twinfilin promotes depolymerization at barbed ends. How these distinct activities get integrated within a shared cytoplasm is unclear. Using microfluidics-assisted TIRF microscopy, we find that formin, CP and twinfilin can simultaneously bind filament barbed ends. Three‑color, single-molecule experiments reveal that twinfilin cannot bind barbed ends occupied by formin unless CP is present. This trimeric complex is short-lived (~1 s), and results in dissociation of CP by twinfilin, promoting formin-based elongation. Thus, the depolymerase twinfilin acts as a pro-formin pro-polymerization factor when both CP and formin are present. While one twinfilin binding event is sufficient to displace CP from the barbed-end trimeric complex, ~31 twinfilin binding events are required to remove CP from a CP-capped barbed end. Our findings establish a paradigm where polymerases, depolymerases and cappers together tune actin assembly." @default.
- W4383346740 created "2023-07-07" @default.
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- W4383346740 date "2023-07-06" @default.
- W4383346740 modified "2023-09-26" @default.
- W4383346740 title "Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping protein" @default.
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- W4383346740 doi "https://doi.org/10.1038/s41467-023-39655-3" @default.
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